JEOL 1200EX II.pratical-Digital
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Rumson Fair Haven Reg H *
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Course
101
Subject
Mechanical Engineering
Date
Nov 24, 2024
Type
Pages
3
Uploaded by CoachRiverTiger30
P
RACTICAL E
XAM ON JEOL
1200EX
II
F
OR TEM
L
ABORATORY C
OURSE &
C
HECKLIST OF R
EQUIREMENTS FOR U
SING THE TEM
U
NSUPERVISED
User:
Date:
Examiner:
Users must score 100% to use the JEOL 1200EX II Unsupervised.
Users can be checked out any number of times until her/his score is 100%.
Scope must be used at least once a semester to maintain unsupervised status.
YES
NO
1. Does user know all steps, in order for starting up microscope?
Pre-start Checklist
:
Power On, Vacuum operating at High Vacuum/Green LED Log Book Signed in
LN
2
trap filled
- focusing scope & viewing window covered
Spot Size on 4
Bright-LED On
All deflectors off (Obj-Stig, Cond-Stig Dark Tilt, Bright Tilt and Image-Shift)
Condenser aperture selected
Objective aperture out
Intermediate aperture out
Function to Mag-1
Magnification at 10,000x
Room lights
G
G
2. Can user locate, center and saturate filament correctly?
Beam Generation
:
Filament fully CCW
Step up High Voltage
Bias Set
Brightness (CL#3) Set
Wehnelt (Filament) Image
Beam Alignment
:
Beam Centered, Spot 1 vs Spot 4
Lenses Cleared
Gun Tilts Set
Condenser Lens Stigmated
Condenser Aperture Centered
Filament Saturated
Beam Spread CW
LN
2
trap topped off
G
G
G
G
3. Can user recognize objective aperture alignment, locate and center Aperture?
Beam Centered
Function Diff used to located aperture
Aperture centered
G
G
4. Can user correctly insert and remove specimens?
Magnification reduced [10K or lower]
Beam Spread CW
Specimen trans controls centered at X=0 and Y=0,and tilt=0
E
Loading Specimen Holder
:
Specimen rod holder used
Closed forceps used to open & close securing plate
Grid loaded specimen-side-down
Specimen rod not touched
Lint on o-ring can be removed with naked finger
Inserting Sample
:
Rod inserted with guide pin aligned
Rod pushed and held
while roughing pumps starts
Air lock pumped out TWO
times (for tungsten)
Rod turned 90
E
CW to stop and rod eased into column
Removing sample
:
Specimen trans controls centered at X=0 and Y=0,and tilt=0
E
Specimen properly retrieved from column
Specimen plates closed
Rod returned to air lock
G
G
G
G
G
G
G
G
5. Can user correctly change between grid position 1 & 2?
Magnification reduced [10K or lower]
Beam Spread CW
Current grid to X=0, Y=0 [Page-2]
Function to Low-Mag and Mag at 120X
Swing Objective Aperture out.
Move from one position to the other.
Return: Objective Aperture in, Function to MAG-1, converge the beam.
G
G
6. Can user correct Objective Lens Astigmatism
G
G
7. Can the user correct use the digital camera system?
Camera power ON
Login to workstation and start MaxIM DL 5 software
CCD Temperature to > -9 C
Modify Annotations to show correct Accelerating Voltage
Sample image focused & stigmated
Adjust Brightness to fill the screen
Lift Screen before capture image
Set Magnification and mag bar on image
Convert to Grey Scale: Save image - Check (Contrast /Brightness change)
Screen down
Can Use Video window
G
G
8. Does user know all steps, in order for normal (stand-by) microscope
shutdown?
Specimen removed
Filament fully CCW
High Voltage to 120Kv and ON
Beam Spread CW
Spot Size on 4
Function to MAG-1
Magnification at 10k
Digital Camera Power OFF
Logged out of workstation, Mouse OFF
G
G
9. Does user know Full Shutdown and Startup Procedures?
Full Shutdown
:
When is full shutdown performed?
Normal / Stand-by shutdown completed
High Voltage is OFF
Key switched to OFF
Wait (3 mins) until scope shuts down
Breaker box (wall switch) to OFF
Note posted stating reason for full shutdown
Starting from Full Shutdown
:
Check that reason for full shutdown has been corrected
Breaker box (wall switch) to ON
Check that water recirculator is ON
Turn Power Key switch to Start for 5 seconds and release
Wait 45-60 minutes until Accel Voltage Ready light is Illuminated
Proceed with Normal startup
G
G
10. Does user know correct use of Haskeris water cooling system?
G
G
11. Does user know the emergency procedures?
G
G
12. Final check list
Scope CRT Bright fully CCW
Counter top and Scope console organized (items returned to their places)
Sample rod is in column
Scope is at high vacuum
Log book signed out
Room lights turned off
G
G
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