Chem142_Kinetics1_Report_Gradescope_051621_Mac

A student weighed out 0.150 g of protein powder and dissolved it in 100 mL of water (Solution 1). The student then diluted this solution by transferring 1 mL into a 25 mL flask and diluting with water (Solution 2). Finally, 1 mL of that solution was transferred to a test tube and combined with 4 mL Bradford reagent. The absorbance of the solution in the test tube was 0.144. Assuming that the best fit linear line of the standard curve was y=0.04144x+0.01521 (μgmL), calculate the percent protein by mass in the original protein powder.

A student weighed out 0.150 g of protein powder and dissolved it in 100 mL of water (Solution 1). The student then diluted this solution by transferring 1 mL into a 25 mL flask and diluting with water (Solution 2). Finally, 1 mL of that solution was transferred to a test tube and combined with 4 mL Bradford reagent. The absorbance of the solution in the test tube was 0.187. Assuming that the best fit linear line of the standard curve was y = 0.04144 x + 0.01521 (μ g mL), calculate the percent protein by mass in the original protein powder.

PART ONE: Preparation of FECNS* Solution Prepare the two solutions in table 1 by accurately measuring the required volumes of distilled water and CNS into two labeled test tubes. The absorbance of the solutions must be measured soon after adding the Fe" solution from a buret. TABLE 1 Fe 5 ml 5S mL Solution Distilled Water CNS 2 mL Total Volume 3 ml 2 ml 10 ml 3 mL 10 ml In this part of the experiment you are to explore what the above quote means. As a PRELIMINARY Exercise, formulate a hypothesis as to what would happen under each of the following circumstances: 1. 1ml of 0.0020 M CNS' solution is added to the unused portion of solution 1 in the test tube 2. One drop of 0.20 M CNS solution is added to the unused portion of solution 2 in the test tube.

In a Bradford assay, 11 µl of a protein isolate sample was diluted by adding 21 µl of water prior to the addition 2.0 mL Bradford reagent. Calculate the protein concentration (in mg/mL) of the original protein isolate sample whose absorbance reading at 595 nm is 0.304 after dilution. The protein standard solutions used for the calibration curve of the Bradford assay was constructed using bovine serum albumin (BSA) stock solution whose concentration is 200 µg/mL. The concentration and the corresponding absorbance reading at 595 nm of the protein standard solutions are summarized in the table below: Standard soln # 1 2 13 4 6 conc of BSA 12.00 24.00 36.00 48.00 60.00 (pg/mL) Absorbance at 595 nm 0.000 0.156 0.249 0.328 0.411 0.528 zNote: Final answer format must be x.xxx (three decimal places). Round off only in the final answer. Do not round off in the middle of the calculation.

The concentration of nitrite (NO2) in the unknown sample was determined by a direct (ordinary) calibration plot as shown below: 0.300 Absorbance at 520nm 0.250 0.200 0.150 0.100 0.050 0 0.564 µg/ml 0.200 μg/ml 0.754 µg/ml 0.902 µg/ml y = 0.1793x + 0.0763 R² = 0.9969 0.1 0.2 0.801 μg/ml If the absorbance of the unknown sample was measured 0.238 at 520 nm, what would be the concentration of nitrite? 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1 Nirite (µg/ml)

AP Questions // Questions 10 - 11) A student creates four solutions with varying concentration of FeSCN2 and gathers the following data at 298 K using a spectrophotometer calibrated to 460 nm: [FESCN] Absorbance 1.1 x 10-4 M 0.076 1.6 x 10-4 M 0.112 2.2 x 10-4 M 0.167 2.5 x 10-4 M 0.199 On the axis below, create a Beer's Law calibration plot for [FESCN2*]. Draw a best-fit line through your data points.

Consider the reaction : A+B 2 AB. A calibration plot was constructed using x as the concentration for AB in M and y as the absorbance value of AB, it provided the linear equation : y=25x+0.0008. Determine the concentration of AB in M if the absorbance value is 1.22. Round and report your value to the THIRD decimal place. Numeric value only, no unit. No new data to sa IMG 0915.jpeg e to search PrtScn Home

Based on the data, calculate the concentration of analyte in the ORIGINAL sample in ppm. Report to 1 decimal place Sample: A 10.00 mL aliquot of the unknown was placed in a 100.0 mL volumetric flask and made up to the mark will distilled water. The absorbance of this solution is 0.185. Sample + Spike: A 10.00 mL aliquot of the unknown and a 13.00 mL aliquot of the 46.00 ppm stock were both placed in the same 100.0 mL volumetric flask and diluted to the mark with water. The absorbance of this solution is 0.326.   ( Please type answer note write by hend)

After creating her standard curve for absorption versus the concentration (M) of FD&C Red 40, a student found that her best fit linear line for FD&C Red 40 was y = 2,681x + -0.002. Her Kool-Aid sample had an absorbance of 0.337. If 0.55 grams of Kool-Aid powder was used to prepare an 8-fl oz cup of her assigned flavor, what is the percent by mass of FD&C Red 40 in her 8-fl oz cup?

Using the lab instructions and data table with data, please help me complete part 1 and 2 of my lab worksheet

Sally obtains a standard calibration curve for their assigned food dye by plotting absorbance versus concentration (in M) of known solutions and finds the following slope and intercept:   y = 1.646x + 0.026   Note: Copying and/or posting this or other questions without the express written permission from Dr. Burke and the Department of Chemistry & Biochemistry at the University of Delaware is a violation of intellectual property copyright law.   Sally finds that her original Kool-aid sample is too concentrated, so they dilute it by transferring 13.00 mL of the original Kool-aid sample into a new container and diluting to a total volume of 37.00 mL. If the absorbance of the dilute Kool-aid sample at the appropriate wavelength for their assigned dye is 0.950, determine the concentration (in M) of the assigned food dye in the original Kool-aid sample. Report your answer with three places after the decimal.

Here is the protocol for a UV-Vis spectrophotometer to detect water and chlorine-carbon. 1.Dissolve the water and chlorine-carbon compounds in a solvent, such as water. 2.Prepare a standard solution of known concentration that is similar to the sample being measured. 3.Calibrate the spectrophotometer using the standard solution. 4.Measure the absorbance of the sample using the spectrophotometer. 5.Calculate the concentration of the compounds in the sample using the calibration curve obtained from the standard solution. How is the spectrophotometer calibrated with standard solutions? When is the blank solution placed in the spectrophotmeter?

Table 1.0 of data showing calibration standards prepared by dilution and the absorbance Concentration CuSO4 (M) Volume of 0.5M (ml) Volume of D/Water (ml) Absorbance 0.00 0.10 0.20 0.30 0.40 0.50 II. 0.00 1.00 2.00 3.00 4.00 5.00 5.00 4.00 3.00 2.00 1.00 0.00 1. Construct a graph of concentration against absorbance, clearly show a best fit line. Find regression of your graph. Show how 0.000 0.406 0.638 0.854 1.202 1.276

This was a fractional distillation lab separating cyclohexane and toluene. I know A in the NMR analysis is the aromatic component of toluene but I am having a hard time figuring out the other two peaks. Which one represents the methyl group of toluene and what is the other peak (it might be water or some other impurity)?

Sally obtains a standard calibration curve for their assigned food dye by plotting absorbance versus concentration (in M) of known solutions and finds the following slope and intercept: y = 1.646x + 0.026 Sally finds that her original Kool-aid sample is too concentrated, so they dilute it by transferring 13.00 mL of the original Kool-aid sample into a new container and diluting to a total volume of 37.00 mL. If the absorbance of the dilute Kool-aid sample at the appropriate wavelength for their assigned dye is 0.950, determine the concentration (in M) of the assigned food dye in the original Kool-aid sample. Report answer with three places after the decimal.