ASCP BOC EXAM Study Guide Questions Solved (26)

1

Please propose a chromatography-based strategy to purify human insulin proteins that have been expressed in bacterial cells.

120 100 20 20 bound drug/free drug (%) 60 40 60 80 80 0 0 Scatchard Plot of Drug Binding 20 20 40 60 80 100 120 bound drug (nM)

1) a)What is affinity chromatography and what is it used for? b) A drug called Bamlanivimab is used to treat infections with SARS-CoV-2 (Covid-19).This drug was developed by isolating antibodies from the serum of a patient who recovered from Covid-19.What is possibly on the stationary phase in a column used to isolate antibodies to SARS-CoV-2? How are the antibodies eluted off the column to isolate them for drug development?

+Protein Mr pl А 12,000 28,000 42,000 66,000 (a;) 103,000 (a2) 111,000 3 8.4 C 7.2 9 E 5 F 4.2 Predict how the proteins listed above will behave in a variety of separation and characterization procedures. For each chromatographic technique (you may assume that the protein remains “native," or non-denatured), list the order in which the proteins will elute (come of the end of the column): Size Exclusion Chromatography, pH 6.8 First Last Anion Exchange Chromatography, pH 9, using a salt gradient to elute First Last

EQD₂ = nd* α Assume a/B (d+(a/B)) (2+(a/B)) BED:= (nd)*(1+ α =2 for late effects and a/B 10 for acute effects 1. Using the Equivalent Dose in 2 Gy Fractions equation, compare the following protocols. Which would have better tumor control? Which would have worse late effects? 42 Gy delivered in 12 fractions; 30 Gy delivered in 5 fractions.

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Please just answer this question, I answered the others already Calculate the standard error of regression (SER) for the Lineweaver–Burk slope and the Eadie–Hofstee slope to compare the precision of each. Eadie–Hofstee SER:   it is not 0.128, 0.028, 0.136, or  0.1362 or 0.013.

Heparin is used as an anti-coagulant. A risk of heparin use is thrombocytopenia, or low platelet count. This risk is minimized with the use of low molecular weight heparins (LMWH), therefore it is desirable to separate LMWH from higher molecular weight heparins. The method of choice to do this is molecular exclusion chromatography. Below is a chromatogram from a molecular exclusion chromatographic run. Peaks ranging from A to J are clearly distinguishable. The heparin mixture that was analyzed had anywhere from 6 to 30 repeat units of monomer (where the heparin with 30 repeat units would be roughly five times the size of the heparin with six repeat units). a. Which letter most likely represents the peak with 6 repeat units given these heparin polymers were separated with molecular exclusion chromatography? b. Explain your reasoning describing the mechanism of retention in molecular exclusion chromatography. 100 80 60 60 Relative Abundance 40 40 E GH 20 20 B A 36 38 40 42 44 46 48 50 50…

For what purpose Biuret test is used?