+Protein Mr pI 12,000 28,000 42,000 66,000 (a;) 103,000 (a2) 111,000 А В 8.4 C 7.2 9. E 5 F 4.2 Predict how the proteins listed above will behave in a variety of separation and characterization procedures. For each chromatographic technique (you may assume that the protein remains “native," or non-denatured), list the order in which the proteins will elute (come of the end of the column): Size Exclusion Chromatography, pH 6.8 First Last Anion Exchange Chromatography, pH 9, using a salt gradient to elute

+Protein Mr pI 12,000 28,000 42,000 66,000 (a;) 103,000 (a2) 111,000 А В 8.4 C 7.2 9. E 5 F 4.2 Predict how the proteins listed above will behave in a variety of separation and characterization procedures. For each chromatographic technique (you may assume that the protein remains “native," or non-denatured), list the order in which the proteins will elute (come of the end of the column): Size Exclusion Chromatography, pH 6.8 First Last Anion Exchange Chromatography, pH 9, using a salt gradient to elute

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

See similar textbooks

Similar questions

The following questions regard the chromatograms shown below: Which column gives a greater selectivity factor for compound X and compound Y? Explain your answer.
In an experiment of Reverse Phase chromatography using C-18 stationary phase and a mixture of methanol/ethyl acetate/acetic acid, a researcher tried to separate the 3 compounds shown below. Which compound would have the lowest Rf and which the highest Rf in this experiment? Explain your answer and show any relevant structures.
Need help to interpret this IR
What would happen to the retention time of a compound if the following changes were made? Sketch the chromatogram of this mixture for the following questions (a, b and c). "10א2s 2.5 1.5- 0.5 -05 4.2 4.6 5.2 54 5.6 5.8 Time (s) a. Increase the flow rate of the carrier gas b. Decrease the temperature of the column c. Decrease the length of the column Intensity (arb, units)
Draw a GC chromatogram for a 50:50 and 25:75 mixtures of hexane (bp 68°C) and toluene (bp 111°C).
The substances in the table below were chromatographed on a gel filtration column. Estimate the molecular weight of the unknown. Compound Blue Dextran 2000 Molecular weight 2 x 106 158 000 V. (mL) 17.7 Aldolase 35.6 Catalase 32.3 210 000 Ferritin 28.6 440 000 Thyroglobulin 25.1 669 000 Unknown 30.3
What is the calculated Rf of Brilliant blue (blue) in this problem.
You purified a protein and determined its mass spectrum as below, calculate the molecular weight of the protein, using the peaks at 1001.0 100 1053.6 1053.6 and 1001.0 Da. 953.4 910.1 50 1112.1 m/z Relative abundance (%)
Polarities of analyte functional group increase in the order of hydrocarbon < ethers < esters < ketones < aldehydes < amides < amines < alcohols Arrange the order of elution [from shortest to longest retention time] for the following six compounds from an HPLC C18 column. C2H5OH CH3COCH3 CH3COOH CH3COOC2H5 C2H50C2H5 (СН3)2NH O A. C2H5OH < CH3COOH < (CH3)2NH < CH3COCH3 < CH3COOC2H5
Identify the key fragment structures of 4'-Bromoacetophenone based on the peaks of its attached mass spectrum.
Question 1
Search the literature for an example of a small molecule that has been identified using mass spectrometric techniques a large biomolecule that has been sequenced using mass spectrometric techniques. Describe the isolation (separation) and identification (which ionization source, mass analyzer, and mass detector) techniques were used. Include a summary of the fragmentation pattern (especially how the molecular peak has been identified) for each compound.