+Protein Mr pI 12,000 28,000 42,000 66,000 (a;) 103,000 (a2) 111,000 А В 8.4 C 7.2 9. E 5 F 4.2 Predict how the proteins listed above will behave in a variety of separation and characterization procedures. For each chromatographic technique (you may assume that the protein remains “native," or non-denatured), list the order in which the proteins will elute (come of the end of the column): Size Exclusion Chromatography, pH 6.8 First Last Anion Exchange Chromatography, pH 9, using a salt gradient to elute

+Protein Mr pI 12,000 28,000 42,000 66,000 (a;) 103,000 (a2) 111,000 А В 8.4 C 7.2 9. E 5 F 4.2 Predict how the proteins listed above will behave in a variety of separation and characterization procedures. For each chromatographic technique (you may assume that the protein remains “native," or non-denatured), list the order in which the proteins will elute (come of the end of the column): Size Exclusion Chromatography, pH 6.8 First Last Anion Exchange Chromatography, pH 9, using a salt gradient to elute

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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Question 1
Search the literature for an example of a small molecule that has been identified using mass spectrometric techniques a large biomolecule that has been sequenced using mass spectrometric techniques. Describe the isolation (separation) and identification (which ionization source, mass analyzer, and mass detector) techniques were used. Include a summary of the fragmentation pattern (especially how the molecular peak has been identified) for each compound.
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