+Protein Mr pI 12,000 28,000 42,000 66,000 (a;) 103,000 (a2) 111,000 А В 8.4 C 7.2 9. E 5 F 4.2 Predict how the proteins listed above will behave in a variety of separation and characterization procedures. For each chromatographic technique (you may assume that the protein remains “native," or non-denatured), list the order in which the proteins will elute (come of the end of the column): Size Exclusion Chromatography, pH 6.8 First Last Anion Exchange Chromatography, pH 9, using a salt gradient to elute
+Protein Mr pI 12,000 28,000 42,000 66,000 (a;) 103,000 (a2) 111,000 А В 8.4 C 7.2 9. E 5 F 4.2 Predict how the proteins listed above will behave in a variety of separation and characterization procedures. For each chromatographic technique (you may assume that the protein remains “native," or non-denatured), list the order in which the proteins will elute (come of the end of the column): Size Exclusion Chromatography, pH 6.8 First Last Anion Exchange Chromatography, pH 9, using a salt gradient to elute
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Question
![+Protein
Mr
pl
А
12,000
28,000
42,000
66,000 (a;)
103,000 (a2)
111,000
3
8.4
C
7.2
9
E
5
F
4.2
Predict how the proteins listed above will behave in a variety of separation and
characterization procedures.
For each chromatographic technique (you may assume that the protein remains
“native," or non-denatured), list the order in which the proteins will elute (come of
the end of the column):
Size Exclusion Chromatography, pH 6.8
First
Last
Anion Exchange Chromatography, pH 9, using a salt gradient to elute
First
Last](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F5bbd0284-8cc0-45b5-b1aa-cc674bd15e76%2F8b388830-9c67-4f63-ae4e-3b9f2d29388e%2Fhbs6uc_processed.png&w=3840&q=75)
Transcribed Image Text:+Protein
Mr
pl
А
12,000
28,000
42,000
66,000 (a;)
103,000 (a2)
111,000
3
8.4
C
7.2
9
E
5
F
4.2
Predict how the proteins listed above will behave in a variety of separation and
characterization procedures.
For each chromatographic technique (you may assume that the protein remains
“native," or non-denatured), list the order in which the proteins will elute (come of
the end of the column):
Size Exclusion Chromatography, pH 6.8
First
Last
Anion Exchange Chromatography, pH 9, using a salt gradient to elute
First
Last
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