abnormal urine lab report-2

docx

School

Ashland Community and Technical College *

*We aren’t endorsed by this school

Course

110

Subject

Chemistry

Date

Jun 3, 2024

Type

docx

Pages

Uploaded by destineelmills

Clinical Laboratory Techniques 1- Lab Urinalysis on an Abdominal Urine and Centrifuge Destinee Mills Introduction A centrifuge is used to separate materials that have different densities by applying force and rotating balanced units at various speeds. The force and speed required to separate materials depends on the size and viscosity of the specimens. A clinical centrifuge tends to operate at slower speeds and relative centrifuge force (RCF) and accepts a range of heads and cups to accommodate the various array of laboratory specimen’s containers such as serum tubes, bacteria bottles, blood bags, and urine cups/urinalysis tubes. Specifically, the separation of urine by centrifugation is performed so that the liquid within the urine can be separated to reveal any solid components that may be in the sample such as crystals, blood, or microorganisms. The purpose of this lab was to learn how to use a universal tabletop centrifuge and prepare two abnormal urine samples from that can be evaluated and compared to one another. Materials Universal Centrifuge Disposable gloves Clinitest Dipstick Refractometer Acetone Transfer pipettes Water Urinometer Urine sample C Urine sample B Acetest Tablets Clinitest Tablets 20% Sulfosalicylic Acid Test tubes Microliter Pipette Absorbent Mats Kim Wipes Dry paper towels Procedures 1. Prepare a clinical centrifuge for the use, centrifuge the urine samples, centrifuge a sample, and separate the specimens. 2. Perform a routine urinalysis (physical and chemical) test on abnormal urines C & B and record all results. 3. Perform Clinitest 4. Perform Acetest 5. Perform SSA test 6. Perform Ictotest 7. Properly use a automated instrument to analyze urine specimens (Clinitek) 8. Properly use a refractometer to check the specific gravity of urine. 9. Record all test results as they obtained from the performed tests.

Results Urine # 1- Sample C TEST POS OR NEG OR UNITS COLOR Color N/A Straw Transparency N/A Clear Specific Gravity (Refractometer) N/A 1.010 pH Units= 7 Light green Protein Negative Yellow Glucose 1000 md/dL Dark green Ketone Negative Tan Blood Negative Yellow Bilirubin Negative Tan Urobilinogen Normal Light Pink Nitrite Negative White Leukocyte Esterase Negative White Specific Gravity (Strip) Units= 1.010 Light green Clinitest 2% Dark orange Sulfosalicylic Acid Test Negative No turbidity Acetest Trace Light purple speckles Icotest Negative No color reaction Urine # 2- Sample B TEST POS OR NEG OR UNITS COLOR Color N/A Red Transparency N/A Clear pH Units= 7 Light green Specific Gravity (Refractometer) N/A 1.015 Protein Negative Yellow Glucose Normal Yellow Ketone Trace Pink Blood Negative Yellow Bilirubin ++ Med. Pink Urobilinogen Normal Light Pink Nitrite + Pink Leukocyte Esterase + Lavender Specific Gravity (Strip) Units= 1.015 Army green Clinitest Negative Dark blue Sulfosalicylic Acid Test Negative No turbidity Acetest Trace Light purple speckles Icotest Negative No color reaction

Conclusion All tests were accurately completed as expected, and results were recorded/ interpreted. During this lab, we were able to use a centrifuge and Clinitek machine to determine and interpret the differences in two different abnormal urine samples. A urinalysis, as we learned in the previous lab and lecture, is a test that involves studying the appearance, concentration, and contents of a urine sample. Urinalyses are very common tests that detect a vast range of disorders, diseases, and infections and determine whether the kidneys are functioning the way they should be. With this knowledge, we can assume, that abnormal urine means the samples showed too high or too low of results that are not within the ranges expected. Although both urine samples tested today were classified as abnormal samples, their results still vary from one another in many ways. Sample B- Urine # 2 had a higher detection of bilirubin when sampled where Sample C- Urine # 1 had a negative detection of bilirubin. Abnormal results can detect many different health issues from just a single specimen of urine and are an essential part of a techs job in a clinical laboratory setting.

Your preview ends here

Eager to read complete document? Join bartleby learn and gain access to the full version