Lab 17 Assessing Starch Digestion

11/20/23, 5:46 PM PhysioEx Exercise 8 Activity 1 https://media.pearsoncmg.com/bc/bc_0media_ap/physioex/10/ex8/act1/# 1/3 PhysioEx Lab Report Exercise 8: Chemical and Physical Processes of Digestion Activity 1: Assessing Starch Digestion by Salivary Amylase Name: Kaeyty Richardson Date: 20 November 2023 Session ID: session-c0b2aae5-5a3a-25b3-61f0-81edbac510ca Pre-lab Quiz Results You scored 100% by answering 6 out of 6 questions correctly. Experiment Results Predict Question The substrate for amylase is You correctly answered: starch and carbohydrate. 1 Which of the following is true of enzymes? You correctly answered: Their activity can be affected by temperature and pH. 2 The reagent IKI tests for the presence of You correctly answered: starch. 3 Which of the following is not true of controls? You correctly answered: A negative result with a positive control is required to validate the test. 4 Which of the following is an end product of starch digestion? You correctly answered: maltose and glucose. 5 Hydrolases are enzymes that break down large molecules into smaller subunits through the addition of . You correctly answered: water. 6 Predict Question: What effect do you think boiling and freezing will have on the activity of the amylase enzyme? 1

11/20/23, 5:46 PM PhysioEx Exercise 8 Activity 1 https://media.pearsoncmg.com/bc/bc_0media_ap/physioex/10/ex8/act1/# 2/3 Stop & Think Question Experiment Data Tube № Reagent 1 Reagent 2 Reagent 3 Treatment Time Temp IKI Benedict's 1 Amylase Starch pH 7.0 Buffer Boiled 60 37 + - 2 Amylase Starch pH 7.0 Buffer Frozen 60 37 - ++ 3 Amylase Starch pH 7.0 Buffer None 60 37 - ++ 4 Amylase Deionized Water pH 7.0 Buffer None 60 37 - - 5 Deionized Water Starch pH 7.0 Buffer None 60 37 + - 6 Deionized Water Maltose pH 7.0 Buffer None 60 37 - ++ 7 Amylase Starch pH 2.0 Buffer None 60 37 + + 8 Amylase Starch pH 9.0 Buffer None 60 37 + + Post-lab Quiz Results You scored 100% by answering 4 out of 4 questions correctly. Your answer: Boiling will decrease amylase activity and freezing will increase amylase activity. Tube 2 (amylase, starch, pH 7.0 buffer) appears to have the same amount of starch digested as tube 3 (amylase, starch, pH 7.0 buffer) because You correctly answered: freezing had no effect on the enzyme. 1 Tubes 3 (amylase, starch, pH 7.0 buffer), 7 (amylase, starch, pH 2.0 buffer), and 8 (amylase, starch, pH 9.0 buffer) reveal that You correctly answered: amylase activity was highest at pH 7. 1 This activity includes a number of negative controls. Which tube indicates that the amylase solution was not contaminated with maltose? You correctly answered: tube 4 (amylase, deionized water, pH 7.0 buffer). 2