Lab 4

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Los Medanos College *

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007

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Biology

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Jun 23, 2024

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Uploaded by LieutenantMink2097

Spring 2024 Bio7 Lab 4 — Brine Shrimp & Daphnia they also hatch in the dark? Do they need warm water, or do they require cold conditions? To what extent does salinity play a role in their hatching? What about dissolved oxygen? How might chlorinated water (tap water) have a significant difference on hatching compared to dechlorinated water? Quick Description of Lab Protocol: You will be setting up a control to compare your chosen experimental variable to in order to determine the effects on brine shrimp hatch succegs. You'll get everything set up today, then check back in a week to look for signs of hatching. Thinking Through the Experiment Which question are you most interested in from above paragraph? Have other questions popped up? » Write your testable question below. Do bnne. Shr'\mp PTCDC\" \GS\\— orQorkness 2 With your lab team, you are going to design an experiment that tests the effects of one environmental variable on the hatching of your brine shrimp. » Which environmental condition will represent your test variable? L;Sh’r exposue How will you know your brine shrimp hatched/did not hatch because of your chosen test variable? In this experiment, we want everything (except the test variable) to be the same between the control group and the experimental group. > How will you make sure your experiment is as controlled as possible? Describe your experiment below. -cistance fom lomp Foll coveroge for dork petn digh -place 2 ASheS inSame locakion » What is your hypothesis? How do you think your altered environmental condition will affect hatching? Try writing your hypothesis in an If... then... because... format. Ex: /f we put 1 brine shrimp group in cold water and one in room temperature water (control), then | think fewer eggs will hatch in the cold water because cold slows down the hatching process. I.(} oJe PU‘\' 1 bne Shn np Qroup N WohY ond one inthe dork, the. A0k one ool e lels \i\‘e\l\w hodeh oS v coond \ock neceafonty Wphn enonoy. A, IS} afl

Spring 2024 Bio7 Lab 4 — Brine Shrimp & Daphnia Setting up the Experiment 1. First, set up your control group petri dish. Label one 60mm petri dish “Bio7 Control,” along with your group’s initials. Transfer 10mLs of the mixed marine salt solution into the petri dish. (If salt solution is not pre-mixed, the ratio is 12g [1 Tbsp] of marine salt to 500mL of dechlorinated water) 2. Now, set up your experimental group petri dish. Label your dish “Bio7 Exp,” along with your group’s initials. Alter your water by changing your test variable put 10mL of altered water into your other petri dish. 3. Mark 2 toothpicks at an equal point (6mm from the end) so that each can be used to transfer a relatively equivalent number of eggs. a. For your control, wet a toothpick in the marine salt solution and insert it up to the mark into the packet of brine shrimp eggs. The eggs should stick to your toothpick. Transfer to your petri dish by submerging toothpick in petri dish. b. Repeat these steps for your experimental petri dish using your other toothpick (try to keep everything except your test variable the same!) 4. Cover each petri dish with a lid and seal with tape. Tape the 2 dishes together and put on cart, making sure they are clearly labeled. Record any notes about your set-up here. Did anything unexpected come up? mushlq 30-40 e%g Dock-¥infbil Lignt=lamp (far awoy) \ 5. Allow your dishes to sit for a week. We’'ll check back on them next week! 6. Examine your petri dishes with a magnifying lens or dissecting microscope and record your observations. You may see 1) live brine shrimp, 2) dead or immature brine shrimp and/or 3) unhatched eggs, as shown in below pictures: After 48 hours students may observe a)live brine shrimp b) dead or immature brine shrimp and c) unhatched eggs. igure 2: http://pubs.ninaa.nih.goy/publications/Science BetterSafe ThanSorry/Instru 7. What did you see in each of your petri dishes? Collect your data here: ie)| Treatment: (Dork) Lmovirg ovouedh hokcheds 1ot mown \oke V&N& [nok lots. egas presen Movingdy w

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