BIOS251 W5 Lab Instructions(1)
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Chamberlain University College of Nursing *
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251
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Biology
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Jun 19, 2024
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Week 5 Lab Instructions Integumentary System
Activity
Deliverable
Points
Part A
Activity: Observing Prepared Slides of Epithelium
6
Part B
Activity: Observing Prepared Slides of Dermis
6
Part C
Activity: Prepping/Observing Wet Mount of Cheek Cells
6
Part D
Lab Worksheet (Questions)
12
References:
1.
Saladin
Anatomy & Physiology: The Unity of Form and Function
Part A
Step 1: Read the Entire Lab Packet
1.0
– Read through the laboratory packet – SEE ATTACHED SHEETS
Part A
Step 2: Come to the Lab with Proper PPE
BACKGROUND:
The integument contains multiple layers (epidermis, dermis, and hypodermis) and various cell types in each layer. The outer layer contains five different cell types: stratified squamous epithelium, keratinocytes, melanocytes, Langerhans cells, and Merkel cells. The middle layer of the integument is referred to as the dermis, which is composed of connective and vascular tissue. The dermis and epidermis form the cutaneous membrane. Beneath the dermis lies the subcutaneous or hypodermal layer,
which consists of loose connective tissue, specifically adipose tissue.
PURPOSE:
In this lab, you will be asked to observe prepared slides of the epithelium and dermis of human skin. You will also prepare a wet-mount of cheek cells and observe them under the microscope. Finally, you will demonstrate your knowledge by completing the Lab Worksheet.
MATERIALS:
A copy of the lab report for each member of your group
A writing utensil
Prepared slides of epithelium, connective or vascular tissue
Crystal Violet stain
DI water
Glass slides and cover slips
Microscope
BIOS251
Week 5 Lab Instructions
Page 1 of 7
Immersion Oil
Lens wipes
Toothpicks PREPARATION:
Read your lab in its entirety before coming to class.
Clear your workstation of all unnecessary materials. Book bags and or purses should be hung on hooks or places at the front of class. Make sure all other unnecessary materials (coats, drink containers, unused textbooks, etc.) are all stored and placed in a safe area out of the way.
Obtain all materials listed above.
Familiarize yourself with your lab materials.
Follow the directions of the packet and as presented by your instructor.
Students must wear safety eyewear, gloves and lab coat at all times.
Used glass slides and cover slips are discarded in waste glass container
Be aware of the instructions for documenting your lab work. You will be performing the lab in a group but will each be responsible for recording your own data and creating your own lab report.
ACTIVITY: Part A - Observations of Epithelium
We will observe cells in the most superficial layer of integument in this activity
Method:
1.
Obtain the preserved slide of epithelial cells. Your instructor may have one or more slides for you to observe.
2.
Use Appendix A to follow instructions on using a Microscope.
3.
Observe the slide under 10X, 40X and then using 100X objective lenses, following the directions
from your instructor.
Note to Students:
Don’t forget to use oil with 100X lens only. After completing the observation, clean the oil immersion lens with lens paper to remove oil. Be careful to not contaminate other objective lenses (4X, 10X, 40X) with oil.
4.
Record and describe your observations made using a low-power magnification (10x) and high-
power magnification as directed by your instructor (40x or 100x). Make sure to note as many details as possible, and include the magnification used.
BIOS251
Week 5 Lab Instructions
Page 2 of 7
Observation: Epithelium 40X
100X
4 x 40 x
Activity: Part B – Observation of the Dermis We will observe the cells in the middle layer of integument in this part of the lab.
Method:
1.
Obtain the preserved slide of connective and/or vascular cells. Your instructor may have one or more slides for you to observe.
2.
Note to students: Use Appendix A to follow instructions on using a Microscope. 3.
Observe the slide under 10X, 40X and 100X objective lenses as directed by your instructor. Don’t forget to use oil with 100X lens.
Note to Students:
Don’t forget to use oil with 100X lens only. After completing the observation, clean the oil immersion lens with lens paper to remove oil. Be careful to not contaminate other lenses (4X, 10X, 40X) with oil.
4. Record and describe your observations made using a low-power magnification (10x) and high-power magnification as directed by your instructor (40x or 100x). Make sure to note as many details as possible, and include the magnification used.
BIOS251
Week 5 Lab Instructions
Page 3 of 7
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Related Questions
I need help answering this anatomy question
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Case Study Assessment Task: Below are 3 case studies. You need to read these and based on the situation/case provided, identify the anatomy/physiology involved as related to the Integumentary System, what is going wrong or has occurred, and how that impacts the Integumentary System specifically. You also need to provide an explanation for how the Integumentary System responds to the situation.
1) Patient Name: Alex Turner
Age: 20
Background: Alex moved from a sunny coastal town to a cold, northern city for college. They have been experiencing fatigue, muscle weakness, and recurrent skin issues since the move.
Explain the role of Vitamin D in skin health and overall well-being.
Analyze the effects of Vitamin D deficiency on the integumentary system.
Explain Vitamin D synthesis in UV exposure and how that has impacted Alex in his relocation.
Discuss the impact of relocation on melanin production as well.
2) Donna was riding her horse on a warm sunny day (101 degrees Fahrenheit). Donna…
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H Week 5: Epithelial and Connectiv
H Take Test: Lab Quiz 3-2020 Fall X
G You look under a microscope at
x +
A https://bbhosted.cuny.edu/webapps/assessment/take/launch.jsp?course assessment id3_1640978_1&course_id%3_190070m
Remaining Time: 51 minutes, 35 seconds.
* Question Completion Status:
3
6
10
11
12
13
14
15
16
17
18
19
21
22
23
24
25
26
QUESTION 5
Which type of epithelia would make up an organ that needed to be able to expand to many times it's normal size?
O Pseudostratified columnar
O Simple cuboidal
O Transitional
O Simple squamous
QUESTION 6
You look under a microscope at 400X magnification and see an empty space lined with multiple layers of cells with flattened nuclei, and a large amount
of cell free protein facing the empty space. This would be classified as:
O Transitional epithelium
O Simple squamous epithelium
O Non- keratinized squamous epithelium
O This is not epithelial tissue.
O Keratinized stratified squamous epithelium
Save All Answers
Click Save and Submit to…
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Please answer all questions thank you
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Please do it quickly questions fast
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Please answer all thank you so much!!!
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ground substance
melanocyte
elastin
collagen
suaait lated bfin bu
reticular fibres
macrophage
fibroblast
Stipe
FITOSA
ESTIS For
blood vessels
mast cell
mesenchymal
cell
adipocyte
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1:24 PM Mon Sep 20
=
You
Foundations to Human Anatomy and
Physiology (w/Laboratory)-2223-15
Worksheet 2- Body Tissues- 12-Alpert
Due Today at 11:59 PM
Bate
HOTEL
Dashboard
Calendar
VIRTUAL-LABORATORY-ACTIVITY-ON-TISSUES.pdf
REVIEW QUESTIONS:
1. How is the structure of the epithelial tissue related to the function of the organ? Give one
specific example of this.
2. Why are blood, bone and cartilage considered as specialized connective tissue?
3. How is the structure of the neuron related to the function of the Nervous tissue in
To Do
Notifications
Inbox
66%
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Required information
Biophoto Associates/Science Source
Where are the cutaneous blood vessels and nerve fibers located in this picture?
Multiple Choice
Nerve fibers are only found in the dermis. Blood vessels are found in both the epidermis and dermis.
Both are in the dermis. Neither one is found in the epidermis.
Both are found in all skin layers.
Neither the dermis nor the epidermis contains nerve fibers. Only the dermis contains blood vessels.
Blood vessels are found only in the dermis. Both the dermis and the epidermis contain nerve fibers.
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Post-Lab Questions
1. Label the arrows in the following slide image:
B.
Human Skin 400X.
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Activity 3: Short Essay
Directions: Choose two questions to answer. You may use a separate sheet to complete the task.
1. Ascorbic acid (Vitamin C) is a soluble vitamin in the human body. Justify your answer.
2. Why can termites eat wood barks and other thick and hard parts of plants? Explain your answer.
3. Is using steroids good in the human body? Why or why not?
4. What is the difference between DNA and RNA?
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CW7 Due 3/1 X
C Chapter 5: In x
M Inbox (3,574) X
M Inbox (244)
M Midterm ma X
b Search result x
G how to scree
+
https://peralta.instructure.com/courses/39459/quizzes/131066
B
MERRITT
|COLLEGE
Account
Dashboard
Courses
Skin Drawing 1
Calendar
Using Skin Drawing 1, match the tissue types on the right
column to the lettered regions in the left column:
Inbox
A
keratinized stra
History
В
dense irregular
Studio
C
areolar connect
Starfish
D
Help
reticular conne
3:58 PM
O Type here to search
ENG
3/22/2021
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ble
ru
DUNC
тепон, пратен
2. Complete the labeling activity in FIGURE 4.9 by identifying the tissues and structures indicated.
tc
it
1.
(a)
FIGURE 4.9 Micrographs of tissue samples.
118
LAB EXERCISE 4 Histology
1. Tissue:
- 2.
2. Fiber:
3.
3. Fiber:
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Part G: Post-lab Questions
1. Fill in the blanks with a correct body position term.
a. The elbow is
b. The skull is
C. The feet are
d. The pinky finger is
to the fingernails.
to the brain.
to the head.
to the thumb.
e spine iS
to the sternum (breastbone).
2. Describe or sketch what a pickle might look like after it is cut with a transverse section.
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QUESTIONS
1. Using a reference, find the function of simple cuboidal epithelial tissue in humans and
one location where you would find it. Include source.
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Pre - lab Activity 3
The
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PATHOLOGY № 10
TASK No 1
A patient suffers from chronic bronchitis for 40 years. At the
next examination the doctor, in addition to antibiotic therapy,
appointed a physiotherapeutic treatment - paraffin application (hot
wax) on the chest. After performing this procedure, the patient
complained of a burning sensation in the area of paraffin
application. On examination: the skin of the chest is red, dry and
hot to the touch.
Questions:
6. What is the biological significance of this disorder for the
patient?
7. List the principles of correction of the observed disorder of
regional circulation.
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Questions no 5......label 5,4,3,2,1, asap
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n
a
4
6.
(c) Photomicrograph (400x).
FIGURE 4.8 (continued)
Post-lab ASSESSMENT 4Bo
7.
6. Structure:
7. Tissue:
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- I need help answering this anatomy questionarrow_forwardCase Study Assessment Task: Below are 3 case studies. You need to read these and based on the situation/case provided, identify the anatomy/physiology involved as related to the Integumentary System, what is going wrong or has occurred, and how that impacts the Integumentary System specifically. You also need to provide an explanation for how the Integumentary System responds to the situation. 1) Patient Name: Alex Turner Age: 20 Background: Alex moved from a sunny coastal town to a cold, northern city for college. They have been experiencing fatigue, muscle weakness, and recurrent skin issues since the move. Explain the role of Vitamin D in skin health and overall well-being. Analyze the effects of Vitamin D deficiency on the integumentary system. Explain Vitamin D synthesis in UV exposure and how that has impacted Alex in his relocation. Discuss the impact of relocation on melanin production as well. 2) Donna was riding her horse on a warm sunny day (101 degrees Fahrenheit). Donna…arrow_forwardH Week 5: Epithelial and Connectiv H Take Test: Lab Quiz 3-2020 Fall X G You look under a microscope at x + A https://bbhosted.cuny.edu/webapps/assessment/take/launch.jsp?course assessment id3_1640978_1&course_id%3_190070m Remaining Time: 51 minutes, 35 seconds. * Question Completion Status: 3 6 10 11 12 13 14 15 16 17 18 19 21 22 23 24 25 26 QUESTION 5 Which type of epithelia would make up an organ that needed to be able to expand to many times it's normal size? O Pseudostratified columnar O Simple cuboidal O Transitional O Simple squamous QUESTION 6 You look under a microscope at 400X magnification and see an empty space lined with multiple layers of cells with flattened nuclei, and a large amount of cell free protein facing the empty space. This would be classified as: O Transitional epithelium O Simple squamous epithelium O Non- keratinized squamous epithelium O This is not epithelial tissue. O Keratinized stratified squamous epithelium Save All Answers Click Save and Submit to…arrow_forward
- ground substance melanocyte elastin collagen suaait lated bfin bu reticular fibres macrophage fibroblast Stipe FITOSA ESTIS For blood vessels mast cell mesenchymal cell adipocytearrow_forward1:24 PM Mon Sep 20 = You Foundations to Human Anatomy and Physiology (w/Laboratory)-2223-15 Worksheet 2- Body Tissues- 12-Alpert Due Today at 11:59 PM Bate HOTEL Dashboard Calendar VIRTUAL-LABORATORY-ACTIVITY-ON-TISSUES.pdf REVIEW QUESTIONS: 1. How is the structure of the epithelial tissue related to the function of the organ? Give one specific example of this. 2. Why are blood, bone and cartilage considered as specialized connective tissue? 3. How is the structure of the neuron related to the function of the Nervous tissue in To Do Notifications Inbox 66%arrow_forwardRequired information Biophoto Associates/Science Source Where are the cutaneous blood vessels and nerve fibers located in this picture? Multiple Choice Nerve fibers are only found in the dermis. Blood vessels are found in both the epidermis and dermis. Both are in the dermis. Neither one is found in the epidermis. Both are found in all skin layers. Neither the dermis nor the epidermis contains nerve fibers. Only the dermis contains blood vessels. Blood vessels are found only in the dermis. Both the dermis and the epidermis contain nerve fibers.arrow_forward
- Post-Lab Questions 1. Label the arrows in the following slide image: B. Human Skin 400X.arrow_forwardActivity 3: Short Essay Directions: Choose two questions to answer. You may use a separate sheet to complete the task. 1. Ascorbic acid (Vitamin C) is a soluble vitamin in the human body. Justify your answer. 2. Why can termites eat wood barks and other thick and hard parts of plants? Explain your answer. 3. Is using steroids good in the human body? Why or why not? 4. What is the difference between DNA and RNA?arrow_forwardCW7 Due 3/1 X C Chapter 5: In x M Inbox (3,574) X M Inbox (244) M Midterm ma X b Search result x G how to scree + https://peralta.instructure.com/courses/39459/quizzes/131066 B MERRITT |COLLEGE Account Dashboard Courses Skin Drawing 1 Calendar Using Skin Drawing 1, match the tissue types on the right column to the lettered regions in the left column: Inbox A keratinized stra History В dense irregular Studio C areolar connect Starfish D Help reticular conne 3:58 PM O Type here to search ENG 3/22/2021arrow_forward
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SEE MORE QUESTIONS
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Recommended textbooks for you
- Essentials of Pharmacology for Health ProfessionsNursingISBN:9781305441620Author:WOODROWPublisher:Cengage
- Understanding Health Insurance: A Guide to Billin...Health & NutritionISBN:9781337679480Author:GREENPublisher:Cengage
Essentials of Pharmacology for Health Professions
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ISBN:9781305441620
Author:WOODROW
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Health & Nutrition
ISBN:9781337679480
Author:GREEN
Publisher:Cengage