BIO201 Lab 4

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Lab 4 Diffusion and Osmosis BIO201L Student Name: Kadesha Alawar-Taylor. Access Code (located on the lid of your lab kit): AC-920H4U. Lab Report Format Expectations Utilize college level grammar and professional formatting when completing this worksheet. Submissions without proper formatting, all required photos or sufficient responses will be rejected. Pre- lab Questions 1. Define the following terms. a. hypertonic solution: Hypertonic solution is one with a higher solute concentration than the solute concentration on the opposite side of the permeable embrane. b. hypotonic solution: A solution with a lower solute concentration than the solute concentration on the opposite side of the permeable membrane. c. isotonic solution: a solution with equal solute and concentration of both sides. 2. Define osmotic pressure. Osmotic pressue is the force required to prevents osmosis or lyse of cells. It is the pressure in cels due to the concentration levels of solutes and solvents. 3. Explain what would happen to a cell placed in a hypertonic solution. Because the concentration os olutes is greater than the concentration of solvents in the cell, if a cell was placed into a hypertonic soluton, the water from nside the cell would diffuse to the outside and cause the cell to shrink.

Lab 4 Diffusion and Osmosis BIO201L EXPERIMENT 1: DIFFUSION THROUGH A LIQUID Introduction Questions 1. State the molecular weight of the blue dye in grams per mole. Click here to enter text. 2. State the molecular weight of the red dye in grams per mole. Click here to enter text. 3. Why does this experiment require two dyes of different molecular weights? Answer this question, ensuring you explain the purpose of these two dyes in relation to the experiment. Click here to enter text.

Lab 4 Diffusion and Osmosis BIO201L Data and Observations Record the diameters you observed for both dyes in the table below. Table 1: Rate of Diffusion in Corn Syrup Time (sec) Blue Dye Red Dye 10 Input Input 20 Input Input 30 Input Input 40 Input Input 50 Input Input 60 Input Input 70 Input Input 80 Input Input 90 Input Input 100 Input Input 110 Input Input 120 Input Input Calculate the speed of diffusion and present this, along with the required data, for both dyes below in Table 2. To calculate the hourly diffusion rate, multiply the total distance diffused by 30 . Table 2: Speed of Diffusion of Different Molecular Weight Dyes Structure Molecular Weight Total Distance Traveled (mm) Speed of Diffusion (mm/hr)* Blue Dye Input Input Input Red Dye Input Input Input Results and Discussion 1. Examine the plot below. How well does it match the data you took in Table 1?

Lab 4 Diffusion and Osmosis BIO201L Click here to enter text. 2. Which dye diffused the fastest? Click here to enter text. 3. How does the rate of diffusion correspond with the molecular weight of the dye? Click here to enter text. 4. Does the rate of diffusion change over time? Why or why not? Click here to enter text. EXPERIMENT 2: DIFFUSION - CONCENTRATION GRADIENTS AND MEMBRANE PERMEABILITY Introduction Questions 1. What is the purpose of Step 5 in this experiment? Click here to enter text. 2. Why is it necessary to have positive and negative controls in this experiment? Click here to enter text.

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