Lab Practical Review -spring2024

docx

School

Gwinnett Technical College *

*We aren’t endorsed by this school

Course

1100

Subject

Biology

Date

Jun 2, 2024

Type

docx

Pages

Uploaded by ProfCamel3702

Biology 1107K Lab Practical Review Spring 2022 Practical Review (Overall suggestion):  Re-READ every narrative section, re-ANALYZE the data, as well as every assignment question for the labs completed during the semester.  Be able to CRITICALLY THINK about all collected data and analysis completed in every assignment sheet.  Know how to correctly use all the equipment in lab: pipettors, pipets, spectrophotometer etc. Lab Safety procedures  Review handout about safety that is on D2L.  Do you know what is or is not allowed in lab? Scientific method  Know the steps of the scientific method and all of the terms associated (groups, variable, controls, etc.)  Accurately graph data.  If you are given a scenario, can you plan an experiment correctly? Or understand the provided data?  Identify the independent and dependent variable in an experimental design. Identify them from a graph. Measurement & BioMath  Convert units within the metric system. Milli, micro, etc.  Design how to prepare dilutions eg. 1:5, 1:10, 1:3  Design how to prepare serial dilutions eg. 1:10,000, 1: 1,000,000  Design a dilution series eg. A 1:2 dilution series or a 1:5 series  Know how to use C 1 V 1 = C 2 V 2 Yeast survival curves  Explain the basic exposure of yeast to UV light experiment.  Analyze the data curve and determine the lethal dose at 50% of the population.  Given data, prepare a yeast survival curve. Interpret a yeast survival curve.  Design an experiment to test a factor for UV protection. Identify the independent, dependent variables and what should remain constant across the experiment. Starch experiments  What is the purpose of iodine?  What is the purpose of starch?  What are starch standards? What do you use them for?  How do you make and use a standard curve?  What is qualitative data vs quantitative data?  In the starch experiments, what do you blank the spectrophotometer with?  In the starch experiments, which is darker, higher or lower starch concentrations?  What would be appropriate controls?  What factors affect enzyme activity?  When you increase the amount of enzyme, what happens?  When you increase the amount of substrate, what happens?  What is enzyme velocity? Why do we care about it? 

Discover more documents: Sign up today!

Unlock a world of knowledge! Explore tailored content for a richer learning experience. Here's what you'll get:

Access to all documents
Unlimited textbook solutions
24/7 expert homework help

Lab Practical Review -spring2024

Related Documents