Campbell Biology, Books a la Carte Plus Mastering Biology with eText -- Access Card Package (10th Edition)
10th Edition
ISBN: 9780133922851
Author: Jane B. Reece, Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, Robert B. Jackson
Publisher: PEARSON
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Textbook Question
Chapter 9.4, Problem 2CC
WHAT IF? Ø In the absence of O2 as in question 1, what do you think would happen if you decreased the pH of the intermembrane space of the mitochondrion? Explain your answer.
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Compare the abundance of cristae in the mitochondria in Fig. 18.11b (677) with those in Fig. 20.21 (760). Is there a difference? What might this reflect in functional terms?
If isolated mitochondria are incubated with asource of electrons such as succinate, but without oxygen,electrons enter the respiratory chain, reducing each of theelectron carriers almost completely. When oxygen is thenintroduced, the carriers become oxidized at different rates(Figure Q14–1). How does this result allow you to order the electron carriers in the respiratory chain? What is theirorder?
You are isolating mitochondria from insect cells and incubating in a test tube with 0.005 M FADH2, 0.05 M ADP and 0.05 M Pi. Assuming, these 3 can enter mitochondria at no cost,no glucose/products of glucose metabolism remain in the isolated mitochondria, and oxygen is present.
Part 1) If all expected reactions go to completion, how much ATP is expected formed?
(0 M)(0.02 M) (0.03 M) (0.05 M) (0.1 M) (0.005 M) (0.01 M) (0.015 M)
Part 2) The ratio of FADH2/FAD at completion of all expected reactions would be?
(2) (>>2) (0) (1)
Part 3) After completion of all expected reactions, ratio of H ion concentration inside vs. outside the mitochondrial inner membrane should be?
(<1) (~1) (>1)(0)
Please provide brief explanation
Chapter 9 Solutions
Campbell Biology, Books a la Carte Plus Mastering Biology with eText -- Access Card Package (10th Edition)
Ch. 9.1 - Prob. 1CCCh. 9.1 - WHAT IF? If the following redox reaction...Ch. 9.2 - VISUAL SKILLS During the redox reaction in...Ch. 9.3 - Prob. 1CCCh. 9.3 - What process in your cells produce the CO2 that...Ch. 9.3 - VISUAL SKILLS The conversions shown in Figure...Ch. 9.4 - WHAT IF? What effect would an absence of O2 have...Ch. 9.4 - WHAT IF? In the absence of O2 as in question 1,...Ch. 9.4 - MAKE CONNECTIONS Membranes must be fluid to...Ch. 9.5 - Consider the NADH formed during glycolysis. What...
Ch. 9.5 - WHAT IF? A glucose-fed yeast cell is moved from...Ch. 9.6 - MAKE CONNECTIONS Compare the structure of a fat...Ch. 9.6 - Prob. 2CCCh. 9.6 - Prob. 3CCCh. 9.6 - VISUAL SKILLS During intense exercise, can a...Ch. 9 - Describe the difference between the two processes...Ch. 9 - Which reactions in glycolysis are the source of...Ch. 9 - What molecular products indicate the complete...Ch. 9 - Briefly explain the mechanism by which ATP...Ch. 9 - Prob. 9.5CRCh. 9 - Prob. 9.6CRCh. 9 - Level 1: Knowledge/Comprehension 1. The immediate...Ch. 9 - Prob. 2TYUCh. 9 - 3. The final electron acceptor of the electron...Ch. 9 - Prob. 4TYUCh. 9 - What is the oxidizing agent in the following...Ch. 9 - When electrons flow along the electron transport...Ch. 9 - Prob. 7TYUCh. 9 - Prob. 8TYUCh. 9 - MAKE CONNECTIONS The proton pump shown in Figures...Ch. 9 - INTERPRET THE DATA Phosphofructokinase is an...Ch. 9 - DRAW IT The graph here shows the pH difference...Ch. 9 - EVOLUTION CONNECTION AIP synthases are found in...Ch. 9 - SCIENTIFIC INQUIRY In the 1930s, some physicians...Ch. 9 - WRITE ABOUT A THEME: ORGANIZATION In a short essay...Ch. 9 - SYNTHESIZE YOUR KNOWLEDGE Coenzyme Q (CoQ) is sold...
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- 12. Use Figure 4 for questions a-c below. 114 ATP synthase matrix intermembrane space Figure 4: Sketch of a mitochondrion https://upload.wikimedia.org/wikipedia/commons/1/1a/Schema mitochondrion basic.svg Bionet, CC BY-SA 4.0 , via Wikimedia Commons a. Add to the sketch to show a H* concentration gradient in an active mitochondrion. (Sketch more H* in the appropriate space. Sketch less H* in the appropriate space.) 4+ b. Sketch an arrow to represent the direction that protons flow (via diffusion) through ATP synthase. c. If the concentration of protons was equal on each side of the membrane, how would that affect the flow of protons through ATP synthase? How would that affect ATP production from glucose?arrow_forwardGive answer of all three subparts with explanation!arrow_forwardPFor each of the following experiments, tell me whether you would expect the rate of O₂ consumption and ATP synthesis to increase, decrease or remain the same (respectively). Assume there is plenty of O₂ around. (a) Succinate is suddenly added to mitochondria that have no other oxidizable substrates (Assume that there are ADP and Pi in mitochondria). (b) ADP is not allowed to pass into the mitochondrial matrix. (c) 2,4 Dinitrophenol (an uncoupler) is added.arrow_forward
- . Aerobic respiration, used by the mitochondria of eukaryotic cells, is best represented by which of the following balanced equations? 6CO2 + 12H2S g C6H12O6 + 6S2 + 6H2O C6H12O6 + 6O2 + 6H2O g 6CO2 + 12H2O C12H22O11 + H2O g C6H12O6 + C6H12O6 6CO2 + 12H2O g C6H12O6 + 6O2 + 6H2O C6H12O6 + C6H12O6 g C12H22O11 + H2Oarrow_forwardanswer question #3arrow_forwardIn the early days of “mitochondriology,” P/O ratios were determined frommeasurements of volume of O2 taken up by respiring mitochondria andchemical assays for disappearance of inorganic phosphate. Now, however, itis possible to measure P/O ratios simply with a recording oxygen electrode.How might this be done?arrow_forward
- answer #2arrow_forwardanswer #1, A-Carrow_forwardco S Question #6. On the right is a schematic showing the structure of complex V (ATP Synthase). The various subunits are labeled. 6A Label the figure to show the location of the mitochondrial matrix αβλαβ Y 6B On the figure indicate the direction of proton flow across the membrane in the direction of ATP synthesis. Make sure to specifically indicate through which portion of the complex the protons move. E Ab, a a cc ccc Inner membrane 6C List the specific subunits in the complex that rotate as protons flow through the complex (select from: a, b2, c, a, ß, y, 8, ɛ) 6D Rotation occurs in 120° steps. Briefly explain why this is consistent with the bind-and-change mechanism that produces ATP. In your answer, make sure to indicate the number of active sites in the complex and how rotation alters their ability to promote catalysis.arrow_forward
- What would be the expected pHs of the matrix and intermembranespace of the mitochondrion? What about the stroma and thylakoidlumen of the chloroplast? Explain your answers.arrow_forwardWhen pure reduced cytochrome c is added to carefully prepared mitochondria along with ADP, P, antimycin A, and oxygen, the cytochrome c becomes oxidized, and ATP is formed, with a P/o ratio approaching 1.0. (a) Indicate the probable flow of electrons in this system. (b) Why was antimycin A added? (c) What does this experiment tell you about the location of cou- pling sites for oxidative phosphorylation? (d) Write a balanced equation for the overall reaction (including cyt c oxidation and ATP synthesis).arrow_forward31arrow_forward
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