Brock Biology of Microorganisms (15th Edition)
15th Edition
ISBN: 9780134261928
Author: Michael T. Madigan, Kelly S. Bender, Daniel H. Buckley, W. Matthew Sattley, David A. Stahl
Publisher: PEARSON
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Chapter 9.4, Problem 1CR
Which genomes are larger, those of chloroplasts or those of mitochondria? How does your genome compare with that of yeast in overall size and gene number?
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Researchers systematically replaced 414 essential genes in yeast with similar genes from humans. Almost half of these transplants (47%) were successful: cells with the humanized gene were able to function and grow. What does this observation tell us about differences between yeast and humans? How might this information be used?
The diagram below represents single-celled
organism A dividing by mitosis to form cells B andC.
B
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Cells A, B, and C all produced protein X. What can
best be inferred from this observation?
A) The gene to produce protein X was passed from
cell A to cells B and C.
B) Cells A, B, and C ingested food containing the
gene to produce protein X.
C) The gene for protein X is found in single-celled
organisms, only.
D) Protein X is found in all organisms.
The figure below shows the life cycle of the fungus Neurospora. The adult stage of the Neurospora is a multicellular haploid:
a. Between which two stages of the Neurospora life cycle do most mitotic cell divisions occur?
b. Neurospora has an arginine amino acid synthesis pathway shown below:
Suppose I have a neurospora strain that has a mutation such that it will not grow unless I supplement the media (food) with arginine (but not with citrulline or ornithine). What gene is mutated? Explain your reasoning.
c. Suppose I take the strain above that only grows with arginine supplements and cross it to a different mutant Neurospora strain that grows with arginine and citrulline supplements but not ornithine supplements. Assming genes A, B and C are unlinked and there is only one mutation per strain:
i) What percentage of the progeny will grow on ornithine?
ii) What percentage on citrulline?
iii) What percentage on arginine?
Show your work for i), ii) and iii). [Can be answered in less…
Chapter 9 Solutions
Brock Biology of Microorganisms (15th Edition)
Ch. 9.1 - How many protein-encoding genes are in the human...Ch. 9.1 - List three examples of how genomics has led to...Ch. 9.1 - What is one discovery resulting from the...Ch. 9.2 - What key molecules are essential for danger...Ch. 9.2 - Prob. 2MQCh. 9.2 - What is the major problem in identifying genes...Ch. 9.2 - How can protein homology assist in genome...Ch. 9.3 - What lifestyle is typical of Bacteria and Archaea...Ch. 9.3 - Prob. 2MQCh. 9.3 - Prob. 3MQ
Ch. 9.3 - Prob. 1CRCh. 9.4 - Prob. 1MQCh. 9.4 - Prob. 2MQCh. 9.4 - Prob. 3MQCh. 9.4 - Which genomes are larger, those of chloroplasts or...Ch. 9.5 - Prob. 1MQCh. 9.5 - Prob. 2MQCh. 9.5 - Prob. 3MQCh. 9.5 - What is the major difference in how duplications...Ch. 9.6 - Which class of genes is rarely transferred...Ch. 9.6 - List the major mechanisms by which horizontal gene...Ch. 9.6 - How might transposons be especially important in...Ch. 9.6 - Explain how horizontally transferred genes can be...Ch. 9.7 - Prob. 1MQCh. 9.7 - Prob. 2MQCh. 9.7 - Prob. 3MQCh. 9.7 - Explain how chromosomal islands might move between...Ch. 9.8 - Prob. 1MQCh. 9.8 - How is a metagenome analyzed?Ch. 9.8 - Prob. 1CRCh. 9.9 - Prob. 1MQCh. 9.9 - Prob. 2MQCh. 9.9 - Prob. 3MQCh. 9.9 - Prob. 1CRCh. 9.10 - Why is the term proteome ambiguous, whereas the...Ch. 9.10 - Prob. 2MQCh. 9.10 - Prob. 3MQCh. 9.10 - Prob. 1CRCh. 9.11 - Prob. 1MQCh. 9.11 - What is a secondary metabolite?Ch. 9.11 - Prob. 1CRCh. 9.12 - How are single cells isolated from a mixed...Ch. 9.12 - Prob. 2MQCh. 9.12 - How can single-cell genomics be used to address...Ch. 9.13 - Prob. 1MQCh. 9.13 - Prob. 2MQCh. 9.13 - Prob. 1CRCh. 9.14 - Prob. 1MQCh. 9.14 - Prob. 2MQCh. 9.14 - Prob. 1CRCh. 9 - Apart from genome size, what factors make complete...Ch. 9 - Describe how one might determine which proteins In...Ch. 9 - The gene encoding the beta subunit of RNA...Ch. 9 - Describe how you could use systems biology to...
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- The figure below shows the life cycle of the fungus Neurospora. The adult stage of the Neurospora is a multicellular haploid. Neurospora has an arginine amino acid synthesis pathway shown below. Suppose I have a Neurospora strain that has a mutation such that it will not grow unless I supplement the media (food) with arginine (but not with citrulline or ornithine). What gene is mutated? Explain your reasoning.arrow_forward#15 & #16arrow_forwardYou isolate a cell from a eukaryotic species where n = 14. The cell contains 28 DNA molecules. choose best answer from the bolded words in each phrase: 1. The cell could/could not be at the end of stage G1 of the cell cycle, as the cell in this stage is diploid/haploid and each chromosome is made of 2/1 DNA molecule(s). 2. The cell could/could not be at the end of stage Metaphase I, as the cell in this stage is diploid/haploid and each chromosome is made of 2/1 DNA molecule(s). 3. The cell could/could not be at the end of stage Prophase II, as the cell in this stage is diploid/haploid and each chromosome is made of 2/1 DNA molecule(s).arrow_forward
- Almost all cells within an animal contain DNA with the same sequence, yet different cells can have very different properties and gene expression patterns. What are the primary mechanisms that facilitate the existence of distinct cell types in eukaryotes?arrow_forwardYou are working with two different yeast cultures to study their genetics. But, you are not sure what the mating type of either of the yeast cultures is. (As a reminder, yeast mating types are either a or alpha). As a test, you mix the two cultures together and then observe them under a microscope. If this is the image that you see, what can you conclude about the mating types of the yeast? Explain your answer, including what type of cell morphology you see on this microscope slide.arrow_forward. In examining Figure 3-19, what do you think is the mainreason for the difference in size of yeast and humanmtDNA?arrow_forward
- If two chromosomes of a species are the same length and have similar centromere placements and yet are not homologous, what is different about them? Choose 1: A) Genes are different B) Alleles are different C) Loci are different D) Nothing is differentarrow_forwardIn Figure 17-25, which species is most closely related tothe ancestral yeast strain? Why are genes 3 and 13 referred to as duplicate?arrow_forwardIt is possible to take the DNA of a gene from any source and place it on a chromosome in the nucleus of a yeast cell. When you take DNA of a human gene and put it into a yeast cell chromosome, the yeast cell can synthesize the human protein. However, when you remove the DNA for a gene normally present on yeast mitochondrial chromosomes and put it on a yeast chromosome in the nucleus, the yeast cell cannot synthesize the correct protein, even though the gene comes from the same organism. Explain. What would you need to do to ensure that such a yeast cell could make the correct protein?arrow_forward
- Consider Molecule X, which is found in all living cells. This molecule is transcribed from a stretch of DNA in the nucleus. Each nucleobase on the DNA produces a matching nucleobase on this molecule. Every 3-base codon specifies an amino acid in a protein. What is the name of X? Your answer should be one word, or a short two- or three-word phrase. Spelling counts. Note: if there is more than one possible answer, separate each answer with a comma. x 5arrow_forwardCompare genome size, gene number, and gene density (a) in the threedomains and (b) among eukaryotes.arrow_forwardWhat is the genetic basis for the bewildering range of sizes and shapes displayed by complex multicellular organisms?arrow_forward
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