Brock Biology of Microorganisms (15th Edition)
15th Edition
ISBN: 9780134261928
Author: Michael T. Madigan, Kelly S. Bender, Daniel H. Buckley, W. Matthew Sattley, David A. Stahl
Publisher: PEARSON
expand_more
expand_more
format_list_bulleted
Concept explainers
Textbook Question
Chapter 9, Problem 2AQ
Describe how one might determine which proteins In Escherichia coli are repressed when a culture is shifted from a minimal medium (containing only a single carbon source) to a rich medium containing many amino acids, bases, and vitamins. How might one study which genes are expressed during each growth condition?
Expert Solution & Answer
Trending nowThis is a popular solution!
Students have asked these similar questions
Describe how one might determine which protein in E. coli are repressed when a culture is shifted from a minimal medium (containing only a single carbon source) to a rich medium containing many amino acids, bases, and vitamins. How might one study which genes are expressed during each growth condition?
Yeast cells are eukaryotes, and they require a functional secretory pathway to grow
and to maintain cellular organization. But surprisingly, when SRP is removed from
yeast cells by deleting the relevant genes, the cells are still alive (although they grow
slowly).
a) How can yeast survive without SRP? Propose two alternative hypotheses.
b) How might electron microscopy of normal and SRP-deficient yeast cells help you
to distinguish between these two hypotheses?
what is the nature and likely location(s) of a mutant that would,
1)allow constitutive expression of the lac gene?
2)prevent the cell from responding to lactose ( genes are not induced when exposed to lactose)?
3) not allow the cell to utilize lactose even when the genes are induced
Chapter 9 Solutions
Brock Biology of Microorganisms (15th Edition)
Ch. 9.1 - How many protein-encoding genes are in the human...Ch. 9.1 - List three examples of how genomics has led to...Ch. 9.1 - What is one discovery resulting from the...Ch. 9.2 - What key molecules are essential for danger...Ch. 9.2 - Prob. 2MQCh. 9.2 - What is the major problem in identifying genes...Ch. 9.2 - How can protein homology assist in genome...Ch. 9.3 - What lifestyle is typical of Bacteria and Archaea...Ch. 9.3 - Prob. 2MQCh. 9.3 - Prob. 3MQ
Ch. 9.3 - Prob. 1CRCh. 9.4 - Prob. 1MQCh. 9.4 - Prob. 2MQCh. 9.4 - Prob. 3MQCh. 9.4 - Which genomes are larger, those of chloroplasts or...Ch. 9.5 - Prob. 1MQCh. 9.5 - Prob. 2MQCh. 9.5 - Prob. 3MQCh. 9.5 - What is the major difference in how duplications...Ch. 9.6 - Which class of genes is rarely transferred...Ch. 9.6 - List the major mechanisms by which horizontal gene...Ch. 9.6 - How might transposons be especially important in...Ch. 9.6 - Explain how horizontally transferred genes can be...Ch. 9.7 - Prob. 1MQCh. 9.7 - Prob. 2MQCh. 9.7 - Prob. 3MQCh. 9.7 - Explain how chromosomal islands might move between...Ch. 9.8 - Prob. 1MQCh. 9.8 - How is a metagenome analyzed?Ch. 9.8 - Prob. 1CRCh. 9.9 - Prob. 1MQCh. 9.9 - Prob. 2MQCh. 9.9 - Prob. 3MQCh. 9.9 - Prob. 1CRCh. 9.10 - Why is the term proteome ambiguous, whereas the...Ch. 9.10 - Prob. 2MQCh. 9.10 - Prob. 3MQCh. 9.10 - Prob. 1CRCh. 9.11 - Prob. 1MQCh. 9.11 - What is a secondary metabolite?Ch. 9.11 - Prob. 1CRCh. 9.12 - How are single cells isolated from a mixed...Ch. 9.12 - Prob. 2MQCh. 9.12 - How can single-cell genomics be used to address...Ch. 9.13 - Prob. 1MQCh. 9.13 - Prob. 2MQCh. 9.13 - Prob. 1CRCh. 9.14 - Prob. 1MQCh. 9.14 - Prob. 2MQCh. 9.14 - Prob. 1CRCh. 9 - Apart from genome size, what factors make complete...Ch. 9 - Describe how one might determine which proteins In...Ch. 9 - The gene encoding the beta subunit of RNA...Ch. 9 - Describe how you could use systems biology to...
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Which of the following statements are true regarding the metabolome, the proteome, the genome, and the transcriptome? The number of genes found in the genome equals the number of different proteins that can be made within any give cell. The number of transcription products equals the number of proteins that can be made from these transcription products. The number of genes found in the genome equals the number of different transcription products that can be made within any given cell. The metabolome is encoded by the genome. None of the above.arrow_forwardConsider the gal10D56 reporter gene. In 300 words or fewer, describe 1) the role of GAL7 in galactose metabolism and its importance for cell function 2) the mutation present in the gal10D56 reporter gene 3) the consequence of this mutation for GAL7 expression in wild type cells, 4) the mechanism by which certain mutations can suppress the effects of gal10D56, and 5) the specific purpose for using this reporter gene.arrow_forwardIn a process of production of a recombinant protein by E. coli cells, it was observed accumulation of acetate in the culture medium. In this situation, it can be said that: (a) certainly the process in question was being conducted in anaerobiosis (B).Acetate accumulation is advantageous for the process as the acetate formation reaction generates 1 molecule of ATP (c)Knowing that decreasing the temperature of the process causes a reduction in the rate of glycolysis, this could be a strategy to reduce the accumulation of acetate (d).the acetate formed can be re-assimilated by the cell if the glyoxylate pathway is activated at some point in the culturearrow_forward
- Bacillus subtilis is known to harbour proA gene which is expressed as protease. Protease is an industrially important enzyme but the yield from Bacillus is not satisfactory. What processes of genetic engineering can you conduct to increase the production of proA gene? Briefly discuss your vision on the context.arrow_forwardI have this strain of e coli. Is P+ o+ Z+ Y+ / I- P+ oC Z- Y+ Will beta-galactosidase and permease be expressed? If they are will they be inducible or constitutive?arrow_forwardYou are interested in the activity and regulation of a protease made by the Gram-positive bacterium Geobacillus stearothermophilus. What would be the purpose of constructing each of the following: a His-tagged protease, a transcriptional GFP fusion to the protease gene, and a translational GFP fusion to the protease gene?arrow_forward
- Identify the most mistaken (wrong) choice: a) Transcription machinery and an enhancer can bind to the chromosome at the same time. b) Organic matters may interfere with heat treatment of bacterial growth control. ( c) Nitrocellulose can be used to filter out microorganisms from a liquid solution. d) Time to kill a bacterial culture is not proportional to the number of microbes in the culturearrow_forwardSalmonella enterica can grow on the lipid breakdown product ethanolamine as a sole carbon and nitrogen source. Ethanolamine is abundant in the mammalian gut, especially during inflammation. You hypothesize that S. enterica has genes encoding a pathway specifically required for growth on ethanolamine. Design a mutant hunt that would allow you to identify Salmonella genes necessary for growth on ethanolamine, and state: • the method of mutagenesis you will use (and why) • are you using a screen, a selection, and/or an enrichment to identify relevant mutants? • the independent and dependent variables • both positive and negative controls • potential outcomes of your experiment, and how you will interpret themarrow_forwardA series of auxotrophic mutants were isolated in Neurospora. Examination of fungi containing these mutations revealed that they grew on minimal medium to which various compounds (A, B, C, D) were added; growth responses to each of the four compounds are presented in the following table. Give the order of compounds A, B, C, and D in a biochemical pathway. Outline a biochemical pathway that includes these four compounds and indicate which step in the pathway is affected by each of the mutations. Compound Mutation number А в С A D 134 276 987 773 772 146 333 123 + + I +arrow_forward
- Chlamydia are pathogenic bacteria that must be grown within a eukaryotic host cell. They rely on the host cell for many nutrients, including nucleotides, lipids, and amino acids. It is thus no surprise that when grown in co-culture with a host depleted of any of these metabolites, chlamydial growth declines. However, high levels of certain amino acids also inhibit chlamydial growth. Specifically, the amino acids leucine, isoleucine, methionine, and phenylalanine slow down the growth by inhibiting the amino acid transporter called BrnQ. On the other hand, if valine is present in the medium these amino acids have no effect on BrnQ. Discuss how this might be possiblearrow_forwardProvide a detailed description of gene expression and control in prokaryotes. Provide a detailed description of proteins critical for this process. (please hand draw a figure showing gene expression and control in prokaryotes and the proteins involved)arrow_forwardBriefly describe how a phosphorelay system and sigma factors are used to control sporulation in B. subtilis. Give one example of posttranslational modification as a means to regulate this process.arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
- Human Anatomy & Physiology (11th Edition)BiologyISBN:9780134580999Author:Elaine N. Marieb, Katja N. HoehnPublisher:PEARSONBiology 2eBiologyISBN:9781947172517Author:Matthew Douglas, Jung Choi, Mary Ann ClarkPublisher:OpenStaxAnatomy & PhysiologyBiologyISBN:9781259398629Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa StouterPublisher:Mcgraw Hill Education,
- Molecular Biology of the Cell (Sixth Edition)BiologyISBN:9780815344322Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter WalterPublisher:W. W. Norton & CompanyLaboratory Manual For Human Anatomy & PhysiologyBiologyISBN:9781260159363Author:Martin, Terry R., Prentice-craver, CynthiaPublisher:McGraw-Hill Publishing Co.Inquiry Into Life (16th Edition)BiologyISBN:9781260231700Author:Sylvia S. Mader, Michael WindelspechtPublisher:McGraw Hill Education
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:PEARSON
Biology 2e
Biology
ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:OpenStax
Anatomy & Physiology
Biology
ISBN:9781259398629
Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:9780815344322
Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:9781260159363
Author:Martin, Terry R., Prentice-craver, Cynthia
Publisher:McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Biology
ISBN:9781260231700
Author:Sylvia S. Mader, Michael Windelspecht
Publisher:McGraw Hill Education
cell culture and growth media for Microbiology; Author: Scientist Cindy;https://www.youtube.com/watch?v=EjnQ3peWRek;License: Standard YouTube License, CC-BY