The gene which is farthest from the his 2. Introduction: In the experiment the his 2 + gene is co transformed with various other genes which gives different rate of cotransformation. The distance between the genes is determined with the help of these given rate of cotransformations.

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Answer 1

Question

Chapter 9.3, Problem 28AQP

(1)

Summary Introduction

To determine:

The gene which is farthest from the his_2.

Introduction:

In the experiment the his₂⁺ gene is co transformed with various other genes which gives different rate of cotransformation. The distance between the genes is determined with the help of these given rate of cotransformations.

(2)

Summary Introduction

To determine:

The genes which are closest to each other.

Introduction:

The rate of cotransformation is used to identify the distance between the genes. The cotransformation is defined as the transformation of more than one gene at the same time.

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a) Calculating Transformation Efficiency For the +DNA/+Amp/+IPTG plate, record the following: Number of transformants (colonies): _________________ Nanograms of plasmid DNA added: 50 ng Final recovery volume: 0.50 mL Volume plated: 0.25 mL Transformation efficiency equation: Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL) b) Using the equation above, calculate the transformation efficiency. c) Describe the success of the transformation efficiency of this demo based on the calculation you did above?

a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates? b) Predict the growth you would expect to see on each of the following plates: – DNA ___________________________________________________________ – DNA/+Amp ______________________________________________________ +DNA/+Amp ______________________________________________________ +DNA/+Amp/+IPTG _________________________________________________

1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids? 2) Calculating Transformation Efficiency For the +DNA/+Amp/+IPTG plate, record the following: Number of transformants (colonies): _________________ Nanograms of plasmid DNA added: 50 ng Final recovery volume: 0.50 mL Volume plated: 0.25 mL Transformation efficiency equation: Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL) 3) Using the equation above, calculate the transformation efficiency. 4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?

Answer 2

Question

Chapter 9.3, Problem 28AQP

(1)

Summary Introduction

To determine:

The gene which is farthest from the his_2.

Introduction:

In the experiment the his₂⁺ gene is co transformed with various other genes which gives different rate of cotransformation. The distance between the genes is determined with the help of these given rate of cotransformations.

(2)

Summary Introduction

To determine:

The genes which are closest to each other.

Introduction:

The rate of cotransformation is used to identify the distance between the genes. The cotransformation is defined as the transformation of more than one gene at the same time.

Expert Solution & Answer

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Check out a sample textbook solution

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Answer 3

Question

Chapter 9.3, Problem 28AQP

(1)

Summary Introduction

To determine:

The gene which is farthest from the his_2.

Introduction:

In the experiment the his₂⁺ gene is co transformed with various other genes which gives different rate of cotransformation. The distance between the genes is determined with the help of these given rate of cotransformations.

(2)

Summary Introduction

To determine:

The genes which are closest to each other.

Introduction:

The rate of cotransformation is used to identify the distance between the genes. The cotransformation is defined as the transformation of more than one gene at the same time.

Expert Solution & Answer

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Check out a sample textbook solution

See solution

Answer 4

Question

Chapter 9.3, Problem 28AQP

(1)

Summary Introduction

To determine:

The gene which is farthest from the his_2.

Introduction:

In the experiment the his₂⁺ gene is co transformed with various other genes which gives different rate of cotransformation. The distance between the genes is determined with the help of these given rate of cotransformations.

(2)

Summary Introduction

To determine:

The genes which are closest to each other.

Introduction:

The rate of cotransformation is used to identify the distance between the genes. The cotransformation is defined as the transformation of more than one gene at the same time.

Expert Solution & Answer

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Check out a sample textbook solution

See solution

Answer 5

Chapter 9.3, Problem 28AQP

(1)

Summary Introduction

To determine:

The gene which is farthest from the his_2.

Introduction:

In the experiment the his₂⁺ gene is co transformed with various other genes which gives different rate of cotransformation. The distance between the genes is determined with the help of these given rate of cotransformations.

(2)

Summary Introduction

To determine:

The genes which are closest to each other.

Introduction:

The rate of cotransformation is used to identify the distance between the genes. The cotransformation is defined as the transformation of more than one gene at the same time.

Answer 6

Chapter 9.3, Problem 28AQP

(1)

Summary Introduction

To determine:

The gene which is farthest from the his_2.

Introduction:

In the experiment the his₂⁺ gene is co transformed with various other genes which gives different rate of cotransformation. The distance between the genes is determined with the help of these given rate of cotransformations.

Answer 7

Chapter 9.3, Problem 28AQP

(1)

Summary Introduction

To determine:

The gene which is farthest from the his_2.

Introduction:

In the experiment the his₂⁺ gene is co transformed with various other genes which gives different rate of cotransformation. The distance between the genes is determined with the help of these given rate of cotransformations.

Answer 8

(2)

Summary Introduction

To determine:

The genes which are closest to each other.

Introduction:

The rate of cotransformation is used to identify the distance between the genes. The cotransformation is defined as the transformation of more than one gene at the same time.

Answer 9

(2)

Summary Introduction

To determine:

The genes which are closest to each other.

Introduction:

The rate of cotransformation is used to identify the distance between the genes. The cotransformation is defined as the transformation of more than one gene at the same time.

Answer 10

Expert Solution & Answer

Answer 11

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Answer 12

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Answer 13

Ch. 9.1 - Prob. 1COQ Ch. 9.1 - Prob. 17AQP Ch. 9.1 - Prob. 1TPSQ Ch. 9.2 - Prob. 1CC Ch. 9.2 - Prob. 2COQ Ch. 9.2 - Prob. 3COQ Ch. 9.2 - Prob. 2TPSQ Ch. 9.3 - Prob. 2CC Ch. 9.3 - Prob. 3CC Ch. 9.3 - Prob. 4CC

The gene which is farthest from the his 2. Introduction: In the experiment the his 2 + gene is co transformed with various other genes which gives different rate of cotransformation. The distance between the genes is determined with the help of these given rate of cotransformations.

To determine: The gene which is farthest from the his2. Introduction: In the experiment the his2+ gene is co transformed with various other genes which gives different rate of cotransformation. The distance between the genes is determined with the help of these given rate of cotransformations.

To determine: The genes which are closest to each other. Introduction: The rate of cotransformation is used to identify the distance between the genes. The cotransformation is defined as the transformation of more than one gene at the same time.

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Chapter 9 Solutions

To determine:

The genes which are closest to each other.

Introduction:

The rate of cotransformation is used to identify the distance between the genes. The cotransformation is defined as the transformation of more than one gene at the same time.