Genetics: A Conceptual Approach 6E w/ SaplingPlus (Six-Month Access)
6th Edition
ISBN: 9781319125929
Author: Benjamin A. Pierce
Publisher: W. H. Freeman
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Question
Chapter 9.2, Problem 3COQ
Summary Introduction
To determine:
The difference between complete medium and minimal medium.
Introduction:
The minimal medium has basic requirements which are needed by bacteria to grow and the complete medium has all the substances which are required for growth and reproduction.
Summary Introduction
To determine:
The way in which the complete media and minimal media, to which one or more nutrients have been added, used to isolate auxotrophic mutants of bacteria.
Introduction:
Nutritional requirements of bacterias and the culture media are defined by microbiologists. The culture media typically contain the essential amino acids, vitamins, minerals and other required ions.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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1)What is the selectable marker in this experiment? How…
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In oxidase test with Pseudomonas aeruginosa, the cell cultures on the slide turn colorless to be
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Chapter 9 Solutions
Genetics: A Conceptual Approach 6E w/ SaplingPlus (Six-Month Access)
Ch. 9.1 - Prob. 1COQCh. 9.1 - Prob. 17AQPCh. 9.1 - Prob. 1TPSQCh. 9.2 - Prob. 1CCCh. 9.2 - Prob. 2COQCh. 9.2 - Prob. 3COQCh. 9.2 - Prob. 2TPSQCh. 9.3 - Prob. 2CCCh. 9.3 - Prob. 3CCCh. 9.3 - Prob. 4CC
Ch. 9.3 - Prob. 5CCCh. 9.3 - Prob. 4COQCh. 9.3 - Prob. 5COQCh. 9.3 - Prob. 6COQCh. 9.3 - Prob. 7COQCh. 9.3 - Prob. 18AQPCh. 9.3 - Prob. 19AQPCh. 9.3 - Prob. 20AQPCh. 9.3 - Prob. 21AQPCh. 9.3 - Prob. 22AQPCh. 9.3 - Prob. 23AQPCh. 9.3 - Prob. 24AQPCh. 9.3 - Prob. 25AQPCh. 9.3 - Prob. 26AQPCh. 9.3 - Prob. 27AQPCh. 9.3 - Prob. 28AQPCh. 9.3 - Prob. 29AQPCh. 9.3 - Prob. 39CQCh. 9.3 - Prob. 3TPSQCh. 9.3 - Prob. 4TPSQCh. 9.3 - Prob. 5TPSQCh. 9.4 - Prob. 6CCCh. 9.4 - Prob. 7CCCh. 9.4 - Prob. 8CCCh. 9.4 - Prob. 8COQCh. 9.4 - Prob. 9COQCh. 9.4 - Prob. 10COQCh. 9.4 - Prob. 11COQCh. 9.4 - Prob. 12COQCh. 9.4 - Prob. 13COQCh. 9.4 - Prob. 14COQCh. 9.4 - Prob. 15COQCh. 9.4 - Prob. 16COQCh. 9.4 - Prob. 30AQPCh. 9.4 - Prob. 31AQPCh. 9.4 - Prob. 32AQPCh. 9.4 - Prob. 33AQPCh. 9.4 - Prob. 34AQPCh. 9.4 - Prob. 35AQPCh. 9.4 - Prob. 36AQPCh. 9.4 - Prob. 37AQPCh. 9.4 - Prob. 38AQPCh. 9.4 - Prob. 6TPSQCh. 9.4 - Prob. 7TPSQ
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