Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
12th Edition
ISBN: 9780134605203
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
bartleby

Videos

Textbook Question
Book Icon
Chapter 9, Problem 1Q

What are the Gram reactions of Clostridium and Bacillus?

What genus has swollen endospores?

Expert Solution & Answer
Check Mark
Summary Introduction

To determine:

The gram reactions of Clostridium and Bacillus.

The genus with swollen endospores.

Introduction:

Gram stain is a staining method that helps in the differentiation of gram-negative bacteria and gram-positive bacteria, by determining the physical and chemical properties of their cell wall. Crystal violet stain is used in this test. Gram positive bacteria has a high affinity for crystal violet when gram's iodine is applied to it. in this test, the decolorizing agent used is acetone or ethyl alcohol. Acetone gets dissolved in the lipids of the cell wall of the gram-negative bacteria, which leads to the leaking out of the crystal-violet complex from the thinner peptidoglycan layer.

Explanation of Solution

When the bacterial cells are in a dormant state, then they form endospores. In dormancy, cells do not undergo metabolism and are said to be in the resting phase. Endospores are formed by bacteria, when they face changed or harsh environment, like, less availability of water, nutrients, and growth temperature.

Clostridium is a gram-positive bacterium and causes botulism in humans. It is an anaerobic bacterium with two forms, endospore form and vegetative form. Clostridium microbe is at rest until it is in a stable and improved environment. Generally, in harsh climates, clostridium survives due to the presence of endospores.

Bacillus is a gram-positive bacterium that is rod-shaped. Generally, bacillus is not harmful to humans. But the most common disease caused by bacillus to humans is anthrax. Moreover, bacillus contains swollen endospores as compared to clostridium.

Conclusion

The gram reactions of Clostridium and Bacillus are gram-positive.

The genus with swollen endospores is bacillus.

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!
Students have asked these similar questions
What are biofertilizers and mention the significance
PCBs and River Otters: Otters in Washington State’s Green-Duwamish River have high levels of polychlorinated biphenyls (PCBs) in their livers. PCBs can bind to the estrogen receptors in animals and disrupt the endocrine system of these otters. The PCBs seem to increase the estrogen to androgen ratio, skewing the ratio toward too much estrogen.     How would increased estrogen affect the river otter population? Based on your reading of the materials in this unit, what factors can affect fertility in humans?   Explain how each of the factors affecting human fertility that you described can disrupt the human endocrine system to affect reproduction.
Other than oil and alcohol, are there other liquids you could compare to water (that are liquid at room temperature)? How is water unique compared to these other liquids? What follow-up experiment would you like to do, and how would you relate it to your life?
Knowledge Booster
Background pattern image
Biology
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.
Similar questions
SEE MORE QUESTIONS
Recommended textbooks for you
Text book image
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:PEARSON
Text book image
Biology 2e
Biology
ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:OpenStax
Text book image
Anatomy & Physiology
Biology
ISBN:9781259398629
Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:Mcgraw Hill Education,
Text book image
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:9780815344322
Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:W. W. Norton & Company
Text book image
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:9781260159363
Author:Martin, Terry R., Prentice-craver, Cynthia
Publisher:McGraw-Hill Publishing Co.
Text book image
Inquiry Into Life (16th Edition)
Biology
ISBN:9781260231700
Author:Sylvia S. Mader, Michael Windelspecht
Publisher:McGraw Hill Education
Bacterial Infections in Humans; Author: Professor Dave Explains;https://www.youtube.com/watch?v=FeFKAl9KyMg;License: Standard Youtube License