Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
12th Edition
ISBN: 9780134605203
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
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Chapter 9, Problem 1CA

Endospores appear as colorless areas in a simple stain and in a Gram stain. Why is it necessary to perform an endospore stain to identity Clostridium difficile in healthcare settings?

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What staining technique is used here? What is the primary stain, decolorizing agent, and counter stain of this technique? Do we heat fix in here? Describe the organism with the blue color. Describe the organism with the bright pink color. Name the genus of bacteria with bright pink.
What is the purpose of iodine in the gram staining process? Explain.
About the Gram staining technique, explain: What is the purpose of using lugol and alcohol in this type of coloring?
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