Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
12th Edition
ISBN: 9780134605203
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
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Chapter 9, Problem 1CA
Endospores appear as colorless areas in a simple stain and in a
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What staining technique is used here? What is the primary stain, decolorizing agent, and counter stain of this technique? Do we heat fix in here? Describe the organism with the blue color. Describe the organism with the bright pink color. Name the genus of bacteria with bright pink.
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Chapter 9 Solutions
Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
Ch. 9 - What are the Gram reactions of Clostridium and...Ch. 9 - How might a capsule contribute to pathogenicity?...Ch. 9 - Prob. 3QCh. 9 - The flagella stain uses crystal violet, as does...Ch. 9 - How would an endospore stain of Mycobacterium...Ch. 9 - What type of culture medium would increase the...Ch. 9 - Prob. 3CTCh. 9 - Prob. 4CTCh. 9 - Endospores appear as colorless areas in a simple...
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- why is it necessary to employ a spore stain rather than rely on diagnosis of sporing from examination of gram stainarrow_forwardWhat stain technique is used here? What is the primary stain, mordant, decolorizing agent, and counter stain of this technique? Do we heat fix in here? Describe the organism with purple color. Describe the organism with pinkish red color.arrow_forwardIs it possible to perform double or triple staining using fluorescent dyes? Explain.arrow_forward
- Describe the bacteria observed in different staining procedures. Please refer to the photo - Endospore Stain - Capsule Stainarrow_forwardWhat's the difference between the gram stain of Bacillus megaterium and Bacillus Subtilis?arrow_forwardWhy is it necessary to perform a negative stain to visualize capsules?arrow_forward
- Which of the following serves as a counterstain for the gram-stain technique? It also happens to be the counterstain for the endospore staining technique.arrow_forwardFor each of the following stains, list each of the chemicals used (in order), briefly describe what you would observe under the microscope, and list examples for each. - Gram stain (list color and example for Gram + and Gram -) - Acid Fast (list color and example for Acid Fast + and -) - Negative Stain (list color of organism stained with negative stain) - Capsule Stain (list color and example of organism with capsule) - Spore stain (list color and example of spore and cell)arrow_forwardDifferentiate the staining procedures. Gram Stain Acid Fast Endospore Capsule Flagella a) Principle b) Characteristic of Bacteria viewed using this stain. (e.g. bacterial type, strain, or targeting particular bacterial feature/s) c) Stain/s Used d) Type of Stain e) Mechanism of Staining (of each stain used) f) Flow of Methodarrow_forward
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