Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
12th Edition
ISBN: 9780134605203
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
expand_more
expand_more
format_list_bulleted
Videos
Textbook Question
Chapter 9, Problem 4Q
The flagella stain uses crystal violet, as does the
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solution
Students have asked these similar questions
Are the structural differences between the cell walls of Gram-positive and Gram-negative bacteria responsible in any way for the Gram stain reaction?
What is the difference between gram positive and gram negative bacteria? Describe how we use this to our advantage in the gram staining process. Hint: Gram positive versus Gram negative!!!
When performing a Gram stain on a Gram-negative bacteria, why does the bacteria stain pink?
Crystal violet stain binds to iodine and remains in the bacterial cell wall because of the thick peptidoglycan layer.
Safranin counterstain gets in the bacteria’s cell wall to stain the cell pink.
The alcohol wash removes crystal violet and iodine from the bacteria.
Crystal violet stain binds to iodine and remains in the bacteria cell wall because of the thick peptidoglycan layer AND Safranin counterstain gets in the bacteria’s cell wall to stain the cell pink.
Safranin counterstain gets in the bacteria’s cell wall to stain the cell pink AND The alcohol wash removes crystal violet and iodine from the bacteria.
Chapter 9 Solutions
Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
Ch. 9 - What are the Gram reactions of Clostridium and...Ch. 9 - How might a capsule contribute to pathogenicity?...Ch. 9 - Prob. 3QCh. 9 - The flagella stain uses crystal violet, as does...Ch. 9 - How would an endospore stain of Mycobacterium...Ch. 9 - What type of culture medium would increase the...Ch. 9 - Prob. 3CTCh. 9 - Prob. 4CTCh. 9 - Endospores appear as colorless areas in a simple...
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- This part of the staining process helps the primary stain to remain in the cell during decolorization. O 1) Secondary stain O 2) primary stain O 3) counterstain O 4) fixing reagent O 5) mordantarrow_forwardWhich Is The More Important Step In Gram Stain?arrow_forwardWhy is it important to perform a gram stain for any type of bacteria? After performing the gram stain method what would be the next step? (Think in terms of the real-world situation) Explain.arrow_forward
- Can the gram stain be observed using a form of microscopy other than light microscopy? Why or whynot?arrow_forward1)What is the natural color of cytoplasm? How is it related to our need to stain bacterial cells? 2) Which of the following is the main goal when staining a culture? A) improve contrast B) identify bacteria C) Visualize bacterial structuresarrow_forwardWhy does the endospore retain the green stain, while the ordinary cells do not?arrow_forward
- How is the India ink capsule stain an example of a negative stain?arrow_forwardThe bacterial cells in the image below were stained with a high concentration of carbolfuschin for an extended period of time (5 minutes) while the slide was heated. The slide was decolorized with a mixture of acid and alcohol. The slide was then counter-stained with methylene blue. What kind of stain does this describe?arrow_forwardExplain biologically and chemically how the Gram Stain helps to distinguish between gram-positive and gram-negative cell structures.arrow_forward
- why does sometimes acid-fast bacteria accept the spore stain and spores accept the acid-fast stain.arrow_forwardJanet is doing a Gram stain on a mixed culture that is known to contain both Gram positive and Gram negative bacteria. After finishing, she realizes she mixed up the safranin and alcohol steps. What is likely to be observed when she views the slide under the microscope? All cells will appear purple, regardless of whether they are actually Gram negative or Gram positive. All cells will appear pink, regardless of whether they are actually Gram negative or Gram positive. Gram negative cells will be clear, Gram positive cells will be purple. Gram positive cells will be clear, Gram negative cells will be pink. Cells will appear clear, regardless of whether they are actually Gram negative or Gram positive.arrow_forwardBasic stains are more effective for bacterial staining than acidic dyes. Why? Which component on the cell wall of acid-fast bacteria prevents acid-alcohol from decolorizing protoplasm?arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
Prokaryotic vs. Eukaryotic Cells (Updated); Author: Amoeba Sisters;https://www.youtube.com/watch?v=Pxujitlv8wc;License: Standard youtube license