Concepts of Genetics (12th Edition)
Concepts of Genetics (12th Edition)
12th Edition
ISBN: 9780134604718
Author: William S. Klug, Michael R. Cummings, Charlotte A. Spencer, Michael A. Palladino, Darrell Killian
Publisher: PEARSON
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Chapter 9, Problem 1PDQ

HOW DO WE KNOW? In this chapter, we focused on extranuclear inheritance and how traits can be determined by genetic information contained in mitochondria and chloroplasts, and we discussed how expression of maternal genotypes can affect the phenotype of an organism. At the same time, we found many opportunities to consider the methods and reasoning by which much of this information was acquired. From the explanations given in the chapter, what answers would you propose to the following fundamental questions?

  1. (a) How was it established that particular phenotypes are inherited as a result of genetic information present in the chloroplast rather than in the nucleus?
  2. (b) How did the discovery of three categories of petite mutations in yeast lead researchers to postulate extranuclear inheritance of colony size?
  3. (c) What observations support the endosymbiotic theory?
  4. (d) What key observations in crosses between dextrally and sinistrally coiled snails support the explanation that this phenotype is the result of maternal-effect inheritance?
  5. (e) What findings demonstrate a maternal effect as the basis of a mode of inheritance?
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In a woman who is a carrier of a mutant OTC deficiency allele, her sons who receive the mutant allele will be affected and her daughters will be carriers who may or may not be symptomatic, depending on random X inactivation in the liver. 1) Is this a single gene inheritance or multifactorial disease? 2) Is there a strong genetic or environmental cause to the development of this disease? If both genetic and environmental causes are implicated, you have to indicate each of them separately.
As a volunteer in a genetics lab, Professor Uhura tasked you with testing the biparental mitochondria inheritance hypothesis in Drosophila for a metabolic phenotype that they are studying. Because you just got started in the lab, the professor tells you are not allowed to use any high-end high-tech resources to perform your experiments and to collect data. The only resources you have access to are the various stocks of Drosophila available in the incubators, the resources to feed the flies and to transfer the flies from vial to vial to perform crosses, and tools to measure the metabolic phenotype of interest.  Describe an experimental approach using crosses you could follow that could potentially provide you with data to support the biparental mtDNA inheritance hypothesis?

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Concepts of Genetics (12th Edition)

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