The number of genome equivalents to be 95% confident of having a clone containing each gene. Introduction: The collection of genomic DNA obtained from one type of organism is referred to as the genomic library. In the genomic library, the specific DNA fragments are present in vectors.

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Chapter 9, Problem 17P

Summary Introduction

To determine:

The number of genome equivalents to be 95% confident of having a clone containing each gene.

Introduction:

The collection of genomic DNA obtained from one type of organism is referred to as the genomic library. In the genomic library, the specific DNA fragments are present in vectors.

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7. Aerobic respiration of a protein that breaks down into 12 molecules of malic acid. Assume there is no other carbon source and no acetyl-CoA. NADH FADH2 OP ATP SLP ATP Total ATP Show your work using dimensional analysis here: 3

For each of the following problems calculate the following: (Week 6-3 Video with 6-1 and 6-2) Consult the total catabolic pathways on the last page as a reference for the following questions. A. How much NADH and FADH2 is produced and fed into the electron transport chain (If any)? B. How much ATP is made from oxidative phosphorylation (OP), if any? Feed the NADH and FADH2 into the electron transport chain: 3ATP/NADH, 2ATP/FADH2 C. How much ATP is made by substrate level phosphorylation (SLP)? D. How much total ATP is made? Add the SLP and OP together. 1. Aerobic respiration using 0.5 mole of glucose? NADH FADH2 OP ATP SLP ATP Total ATP Show your work using dimensional analysis here:

Aerobic respiration of one lipid molecule. The lipid is composed of one glycerol molecule connected to two fatty acid tails. One fatty acid is 12 carbons long and the other fatty acid is 18 carbons long in the figure below. Use the information below to determine how much ATP will be produced from the glycerol part of the lipid. Then, in part B, determine how much ATP is produced from the 2 fatty acids of the lipid. Finally put the NADH and ATP yields together from the glycerol and fatty acids (part A and B) to determine your total number of ATP produced per lipid. Assume no other carbon source is available. 18 carbons fatty acids 12 carbons glycerol . Glycerol is broken down to glyceraldehyde 3-phosphate, a glycolysis intermediate via the following pathway shown in the figure below. Notice this process costs one ATP but generates one FADH2. Continue generating ATP with glyceraldehyde-3-phosphate using the standard pathway and aerobic respiration. glycerol glycerol-3- phosphate…

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Question

Chapter 9, Problem 17P

Summary Introduction

To determine:

The number of genome equivalents to be 95% confident of having a clone containing each gene.

Introduction:

The collection of genomic DNA obtained from one type of organism is referred to as the genomic library. In the genomic library, the specific DNA fragments are present in vectors.

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Answer 3

Question

Chapter 9, Problem 17P

Summary Introduction

To determine:

The number of genome equivalents to be 95% confident of having a clone containing each gene.

Introduction:

The collection of genomic DNA obtained from one type of organism is referred to as the genomic library. In the genomic library, the specific DNA fragments are present in vectors.

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Answer 4

Question

Chapter 9, Problem 17P

Summary Introduction

To determine:

The number of genome equivalents to be 95% confident of having a clone containing each gene.

Introduction:

The collection of genomic DNA obtained from one type of organism is referred to as the genomic library. In the genomic library, the specific DNA fragments are present in vectors.

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Answer 5

Chapter 9, Problem 17P

Summary Introduction

To determine:

The number of genome equivalents to be 95% confident of having a clone containing each gene.

Introduction:

The collection of genomic DNA obtained from one type of organism is referred to as the genomic library. In the genomic library, the specific DNA fragments are present in vectors.

Answer 6

Chapter 9, Problem 17P

Summary Introduction

To determine:

The number of genome equivalents to be 95% confident of having a clone containing each gene.

Introduction:

The collection of genomic DNA obtained from one type of organism is referred to as the genomic library. In the genomic library, the specific DNA fragments are present in vectors.

Answer 7

Chapter 9, Problem 17P

Summary Introduction

To determine:

The number of genome equivalents to be 95% confident of having a clone containing each gene.

Introduction:

The collection of genomic DNA obtained from one type of organism is referred to as the genomic library. In the genomic library, the specific DNA fragments are present in vectors.

Answer 8

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Ch. 9 - Match each of the terms in the left column to the...Ch. 9 - For each of the restriction enzymes listed below:...Ch. 9 - The calculations of the average restriction...Ch. 9 - The DNA molecule whose entire sequence follows is...Ch. 9 - Why do longer DNA molecules move more slowly than...Ch. 9 - Agarose gels with different average pore sizes are...Ch. 9 - The following picture shows the ethidium...Ch. 9 - The linear bacteriophage genomic DNA has at each...Ch. 9 - Consider a partial restriction digestion, in which...Ch. 9 - The text stated that molecular biologists have...

The number of genome equivalents to be 95% confident of having a clone containing each gene. Introduction: The collection of genomic DNA obtained from one type of organism is referred to as the genomic library. In the genomic library, the specific DNA fragments are present in vectors.

Solution Summary: The author explains how to determine the number of independent clones to be screened for the genomic equivalents.

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Chapter 9 Solutions

The number of genome equivalents to be 95% confident of having a clone containing each gene. Introduction: The collection of genomic DNA obtained from one type of organism is referred to as the genomic library. In the genomic library, the specific DNA fragments are present in vectors.

Solution Summary: The author explains how to determine the number of independent clones to be screened for the genomic equivalents.

To determine: The number of genome equivalents to be 95% confident of having a clone containing each gene. Introduction: The collection of genomic DNA obtained from one type of organism is referred to as the genomic library. In the genomic library, the specific DNA fragments are present in vectors.

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Chapter 9 Solutions