EBK GENETICS: FROM GENES TO GENOMES
6th Edition
ISBN: 9781260041255
Author: HARTWELL
Publisher: MCGRAW HILL BOOK COMPANY
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Textbook Question
Chapter 9, Problem 24P
Problem 15 showed part of the sequence of the plasmid vector pMBG36. Suppose you make a genomic library by inserting EcoRI-digested fragments of a genome into EcoRI-digested pMBG36. Write out the sequences of two different primers you could use to sequence (in separate reactions) the two ends of all the clones in the library. These primers should be as long as possible based on the information given.
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FLORES DIology Second Semester Review
7. Chi Square Analysis: wild dumpy male x ebony wild female
F1:
All wild wild
F2:
142 wild wild males
52 wild ebony males
46 dumpy wild males
17 dumpy ebony males
151 wild wild females
47 wild ebony females
51 dumpy wild females
8 dumpy ebony females
What pattern of inheritance is observed for body color?
Autosomal recessive
Autosomal dominant
Sex-linked recessive
Sex-linked dominant
What are the genotypes of the parents in the P generation?
a.
XBYdd x XbXbDD
b. Bbdd x bbDd
c. BBdd x bbDD
d. BBX Y x bbXDY
How many flies in the F2 generation would you expect to express the dumpy wild phenotype?
a.
289.125
b. 97
c. 96.375
d. 293
4. This question focuses on entrainment.
a. What is entrainment?
b. What environmental cues are involved in entrainment, and which one is most influential?
c. Why is entrainment necessary?
d. Assuming that a flash of darkness is an effective zeitgeber, what impact on circadian
rhythms would you expect to result from an event such as the 2024 solar eclipse (assume
it was viewed from Carbondale IL, where totality occurred at about 2 pm)? Explain your
reasoning. You may wish to consult this phase response diagram.
Phase Shift (Hours)
Delay Zone
Advance Zone
Dawn
Mid-day
Dusk
Night
Dawn
Time of Light Stimulus
e. Finally, give a real-world example of how knowledge of circadian rhythms and
entrainment has implications for human health and wellbeing or conservation biology.
This example could be from your reading or from things discussed in class.
Generate one question that requires a Punnet Squre to solve the question. Then show how you calculate the possibilities of genotype and phenotype
Chapter 9 Solutions
EBK GENETICS: FROM GENES TO GENOMES
Ch. 9 - Match each of the terms in the left column to the...Ch. 9 - For each of the restriction enzymes listed below:...Ch. 9 - The calculations of the average restriction...Ch. 9 - The DNA molecule whose entire sequence follows is...Ch. 9 - Why do longer DNA molecules move more slowly than...Ch. 9 - Agarose gels with different average pore sizes are...Ch. 9 - The following picture shows the ethidium...Ch. 9 - The linear bacteriophage genomic DNA has at each...Ch. 9 - Consider a partial restriction digestion, in which...Ch. 9 - The text stated that molecular biologists have...
Ch. 9 - a. What is the purpose of molecular cloning? b....Ch. 9 - a. DNA polymerase b. RNA polymerase c. A...Ch. 9 - Is it possible that two different restriction...Ch. 9 - A plasmid vector pBS281 is cleaved by the enzyme...Ch. 9 - A recombinant DNA molecule is constructed using a...Ch. 9 - Suppose you are using a plasmid cloning vector...Ch. 9 - Prob. 17PCh. 9 - The lacZ gene from E. coli encodes the enzyme...Ch. 9 - Your undergraduate research advisor has assigned...Ch. 9 - Which of the enzymes from the following list would...Ch. 9 - You use the primer 5 GCCTCGAATCGGGTACC 3 to...Ch. 9 - a. To make a genomic library useful for sequencing...Ch. 9 - Problem 15 showed part of the sequence of the...Ch. 9 - Eukaryotic genomes are replete with repetitive...
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- Briefly state the physical meaning of the electrocapillary equation (Lippman equation).arrow_forwardExplain in a small summary how: What genetic information can be obtained from a Punnet square? What genetic information cannot be determined from a Punnet square? Why might a Punnet Square be beneficial to understanding genetics/inheritance?arrow_forwardIn a small summary write down:arrow_forward
- Not part of a graded assignment, from a past midtermarrow_forwardNoggin mutation: The mouse, one of the phenotypic consequences of Noggin mutationis mispatterning of the spinal cord, in the posterior region of the mouse embryo, suchthat in the hindlimb region the more ventral fates are lost, and the dorsal Pax3 domain isexpanded. (this experiment is not in the lectures).a. Hypothesis for why: What would be your hypothesis for why the ventral fatesare lost and dorsal fates expanded? Include in your answer the words notochord,BMP, SHH and either (or both of) surface ectoderm or lateral plate mesodermarrow_forwardNot part of a graded assignment, from a past midtermarrow_forward
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