BIO 1408/09 PKG W/LS CODE
5th Edition
ISBN: 9781260370829
Author: Mader
Publisher: MCGRAW-HILL HIGHER EDUCATION
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Chapter 8.4, Problem 15A
Summary Introduction
Introduction:
Tumor suppressor gene is a gene that regulates cell cycle, DNA repair and apoptosis. These genes reduce the chances of conversion of normal cell to transformed cancerous cell. They are divided into the caretaker genes (associated with DNA damage and repair) and gatekeeper genes (associated with apoptosis).
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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1)What is the selectable marker in this experiment? How…
Based on your results, which suspect's DNA best matches the DNA found at the crime scene?
In oxidase test with Pseudomonas aeruginosa, the cell cultures on the slide turn colorless to be
purple after tetra-methyl-p-phenylenediamine dihydrochloride (TMPD) is added. In the
reaction,
OTMPD is electron acceptor
O cytochrome c is the electron source
oxygen is terminal electron acceptor
OH2 produced is electron donor
Chapter 8 Solutions
BIO 1408/09 PKG W/LS CODE
Ch. 8.1 - Prob. 1LOCh. 8.1 - Prob. 2LOCh. 8.1 - Prob. 3LOCh. 8.1 - Prob. 1CYPCh. 8.1 - Prob. 2CYPCh. 8.1 - Prob. 3CYPCh. 8.1 - Prob. 1ACh. 8.1 - Prob. 2ACh. 8.2 - Summarize the activities that occur in the cell...Ch. 8.2 - Prob. 2LO
Ch. 8.2 - Prob. 3LOCh. 8.2 - Prob. 4LOCh. 8.2 - Prob. 1CYPCh. 8.2 - Compare and contrast what is occurring during each...Ch. 8.2 - Prob. 3CYPCh. 8.2 - Prob. 4CYPCh. 8.2 - Prob. 3ACh. 8.2 - Prob. 4ACh. 8.2 - Prob. 5ACh. 8.2 - Prob. 6ACh. 8.2 - Label the phases of the cell cycle on the...Ch. 8.2 - Prob. 8ACh. 8.2 - Prob. 9ACh. 8.2 - Prob. 10ACh. 8.2 - Prob. 11ACh. 8.3 - Prob. 1LOCh. 8.3 - Prob. 2LOCh. 8.3 - Prob. 3LOCh. 8.3 - Prob. 1CYPCh. 8.3 - Explain how signals are used for cell cycle...Ch. 8.3 - Prob. 3CYPCh. 8.3 - Prob. 12ACh. 8.3 - Prob. 13ACh. 8.4 - Distinguish between proto—oncogenes and tumor...Ch. 8.4 - 2. Explain the role of telomerase in stem cells...Ch. 8.4 - Prob. 3LOCh. 8.4 - Prob. 4LOCh. 8.4 - Prob. 1CYPCh. 8.4 - Prob. 2CYPCh. 8.4 - Prob. 3CYPCh. 8.4 - Which of the following statements is true? a. The...Ch. 8.4 - 15. Which of the following is not a tumor...Ch. 8.5 - Describe the characteristics of cancer cellsCh. 8.5 - 2. Summarize the types of treatment for cancer
Ch. 8.5 - 3. Describe the factors that reduce the risk of...Ch. 8.5 - 1. List the general characteristics of a cancer...Ch. 8.5 - 2. Summarize the ways that cancer may be...Ch. 8.5 - Explain how lifestyle choices may reduce the risk...Ch. 8.5 - 16. Which of the following is not a feature of...Ch. 8.5 - 17. Which of these is a behavior that could help...Ch. 8 - Prob. 1BYBCh. 8 - Prob. 2BYBCh. 8 - Prob. 3BYBCh. 8 - Prob. S3.2BYBCh. 8 - Section 4.4 What are the roles of the...Ch. 8 - Prob. S4.5BYBCh. 8 - Prob. 1TCCh. 8 - Prob. 2TCCh. 8 - Prob. 3TC
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