Microbiology: An Introduction
12th Edition
ISBN: 9780321929150
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case
Publisher: PEARSON
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Textbook Question
Chapter 8, Problem 8R
You are provided with cultures with the following characteristics:
Culture 1: F+, genotype A+ B+ C+
Culture 2: F−, genotype A− B− C−
- a. Indicate the possible genotypes of a recombinant cell resulting from the conjugation of cultures 1 and 2.
- b. Indicate the possible genotypes of a recombinant cell resulting from conjugation of the two cultures after the F+ has become an Hfr cell.
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Students have asked these similar questions
Each of the three different Hfr strains in the table below (A, B and C) arose independently
and possess the same markers. Each of the three Hfr strains was separately mixed with the
same F strain carrying the alternative markers. Conjugation was interrupted at various times
after mixing over a period of 95 minutes. The bacteria were then plated on the appropriate
media and recipients scored for various Hfr markers. The times (in minutes after mixing) at
which recombinants carrying particular donor markers first appear, and therefore the times at
which these first enter the F- cell and then integrate into the recipient genome, are shown
below:
Donor
markers
Time of appearance in minutes
Strain A
Strain B
Strain C
his+
6.5
68.5
29.5
gal+
30.0
46.0
52.0
pro+
34.5
41.5
56.5
met+
58.0
20.0
76.0
mtl+
67.0
11.0
85.0
xyl+
68.5
9.5
86.5
mal+
73.0
5.0
3.0
ser+
81.5
86.5
11.5
tyr+
88.0
78.5
19.5
Draw the genome of each strain showing the location of the markers, the location of F, and
the…
DNA obtalned from the indicated donor strains is used to TRANSFORM the indicated recipient strains. The resulting progeny are plated on minimal medium so that only wild-
type recombinants are scored. The number of wild-types for each cross is given in the chart below. What is the order of the genes?
Donor
Recipient
wid type colonies
a-b- c+
a+ b+ c-
273
a- b+ c-
a+ b-c+
462
a- b+ C+
a+ b-c-
2
a b- C+
a- b+ c+
O 4-b-c
a-o-b
b-a-c
cannot be determined
Question 18 of 26
Match the following Conjugation terms with the most appropriate description in the image:
F- recombinant
F- strain
F+ strain
HFR strain
Chapter 8 Solutions
Microbiology: An Introduction
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Ch. 8 - Match the following terms to the definitions in...Ch. 8 - Feedback inhibition differs from repression...Ch. 8 - Bacteria can acquire antibiotic resistance by all...Ch. 8 - Suppose you inoculate three flasks of minimal...Ch. 8 - Plasmids differ from transposons in that plasmids...Ch. 8 - Mechanism by which the presence of glucose...Ch. 8 - The mechanism by which lactose controls the lac...Ch. 8 - Two offspring cells are most likely to inherit...Ch. 8 - Which of the following is not a method of...Ch. 8 - Nucleoside analogs and ionizing radiation are used...Ch. 8 - Replication of the E. coli chromosome takes 40 to...Ch. 8 - Pseudomonas has a plasmid containing the mer...Ch. 8 - Ciprofloxacin, erythromycin, and acyclovir are...Ch. 8 - HIV, the virus that causes AIDS, was isolated from...Ch. 8 - Human herpesvirus-8 (HHV-8) is common in parts of...
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Two auxotrophic triple Escherichia coli strains (A: met- phe- ade- val+ bio+ thr+ and B: met+ phe+ ade+ val- bio- thr-) are mixed in LB liquid medium, diluted and then spread on LB solid rich medium. Six colonies are observed: Then, replicates are performed on 6 different media (minimum medium + glucose + indicated substances). The results are shown below. Determine the genotype of the 6 colonies observed. Which ones are from strain A? From strain B? Which hypotheses can explain these results and which one do you prefer? met phe val bio Abbreviations: met ade val thr phe ade bio thr Met: methionine; Phe: phenylalanine; Ade: adenine; Val: valine; Bio: biotin; Thr: threonine.arrow_forwardAn Hfr strain that is hisE+ and pheA+ was mixed with a strain thatis hisE− and pheA−. The conjugation was interrupted and the percentageof recombinants for each gene was determined by streakingon a medium that lacked either histidine or phenylalanine. Thefollowing results were obtained:Determine the map distance (in minutes) between these twogenes.arrow_forwardthe circled answer is wrong.arrow_forward
- An Hfr strain that is hisE+ and pheA+ was mixed with a strain thatis hisE− and pheA−. The conjugation was interrupted and the percentageof recombinants for each gene was determined by streakingon a medium that lacked either histidine or phenylalanine. Thefollowing results were obtained:It was found that hisE is 4 minutes away from pabB. The gene pheA was shown to be 17 minutes from pabB. Draw a genetic map describing the locations of all three genes.arrow_forwardDetermine the coefficient of coincidence and the interferencearrow_forward1. Please help answer this question and please show all work on how you got the answers. Thank you so much!! :)arrow_forward
- ) In a conjugation experiment, we used two strains: Hfr: streptomycin sensitive and F-: auxotrophic for isoleucine and threonine, streptomycin resistant. If we interrupt conjugation when only threonine gene has been transferred and spread this mixture on: Minimal media, Minimal media with streptomycin Minimal media with threonine and streptomycin Minimal media with isoleucine and streptomycin LB agar plates with streptomycin On which plates do we expect bacterial growth? Why?arrow_forwardYou have conducted a transposon mutagenesis experiment using the same PRL27 system that was used during your lab exercise. After allowing for conjugation. you plate the conjugation mix on Luria agar and Luria + Kanamycin agar. After incubation, you count the following number of colonies on each type of plate: Media Dilution Number of Colonies Too Many to Luria 10-5 Count 10-6 173 10-7 28 Luria + Kanamycin 100 324 10-1 217 10-2 3 Determine the transformation efficiency. Note: You can enter you answer using long form (e.g. 1500000) or scientific notation using e notation (e.g. 1.5e6). Do not enter units.arrow_forwardSince higher concentration colcemid will result in shorter chromosome, you want to change your protocol and reduce final concentration of colcemid in your 10 ml blood culture from 0.1ug/ml to 0.05ug/ml. how many ul colcemid stock solution with concentration10ug/ml needed to be added in 10ml blood culture?arrow_forward
- First picture: 1. What must be added in a minimal media for the recombinants to grow? 2. What must be excluded in a minimal media for the non recombinants to be killed? Second picture: 1. How is the recipient cell different at time D than it was at time A? Choice 1:It has a greater number of genes Choice 2: It has a greater mass of DNA. Choice 3:It contains different genes Choice 4: It contains bacteriophage DNA. 2. At which time can the recipient cell first be described as "recombinant"? A,B,C, or D 3. Which of the following processes is responsible for the shape of the curve at time B? Choice 1:transduction Choice 2:another choice Choice 3:entry of Hfr DNA into the recipient cell Choice 4:entry of DNA from the recipient cell into the Hfr cellarrow_forwardHave a look at the results in this SDS-PAGE gel. A MW ladder is shown. In the "control" lane, a sample of recombinant protein "LBT3" was loaded. Lane A shows proteins that have been prepared from leukocytes from a normal, healthy individual. Lanes B, C, D, E and F show protein prepared from leukocytes from individuals with lymphoma. What is the best explanation for the result shown for patient D? 200 150 100 75 50 37 20 Weight, kDa MW ladder Control Aarrow_forwardDraw a diagram/figure to explain the conjugation process (e.g. use PowerPoint or draw one by hand and include a photo of it). You should include in the diagram the F- recipient, Hfr Donor and the transconjugant/recombinant recipient. Make sure to include the genes encoding for Leucine, Threonine, Thiamine and Streptomycin resistance in your diagram. How does an Hfr strain of coli transfers chromosomal DNA to an F- strain? What determines how much of the chromosomal DNA is transferred?arrow_forward
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