Microbiology: An Introduction
12th Edition
ISBN: 9780321929150
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case
Publisher: PEARSON
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Chapter 8, Problem 2R
DRAW IT Identify and mark each of the following on the portion of
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Define DNA replication/synthesis and semiconservative replication. In addition, describe and/or define the role(s) of each of the following in the process of DNA replication/synthesis: DNA template strand, 5’ and 3’ ends, DNA helicase, DNA polymerase, single-strand binding proteins, topoisomerase, primase, Okazaki fragments, leading strand and lagging strand.
Describe how replication makes copies of DNA.
Include the following:
helicase
polymerase
primase and primer
ligase
leading strand
lagging strand
base pairing rule (a-t, c-g)
nucelotide
nucleus
semiconservative
Define the following terms related to DNA replication: origin of replication, helicase, single-strand binding proteins, topoisomerase, primase, RNA primer, DNA polymerase III, DNA polymerase I, and DNA ligase.
Chapter 8 Solutions
Microbiology: An Introduction
Ch. 8 - Briefly describe the components of DNA, and...Ch. 8 - DRAW IT Identify and mark each of the following on...Ch. 8 - Match the following examples of mutagens. Column A...Ch. 8 - The following is a code for a strand of DNA. a....Ch. 8 - Identify when (before transcription, after...Ch. 8 - Which sequence is the best target for damage by UV...Ch. 8 - You are provided with cultures with the following...Ch. 8 - Why are mutation and recombination important in...Ch. 8 - NAME IT Normally a commensal in the human...Ch. 8 - Match the following terms to the definitions in...
Ch. 8 - Match the following terms to the definitions in...Ch. 8 - Feedback inhibition differs from repression...Ch. 8 - Bacteria can acquire antibiotic resistance by all...Ch. 8 - Suppose you inoculate three flasks of minimal...Ch. 8 - Plasmids differ from transposons in that plasmids...Ch. 8 - Mechanism by which the presence of glucose...Ch. 8 - The mechanism by which lactose controls the lac...Ch. 8 - Two offspring cells are most likely to inherit...Ch. 8 - Which of the following is not a method of...Ch. 8 - Nucleoside analogs and ionizing radiation are used...Ch. 8 - Replication of the E. coli chromosome takes 40 to...Ch. 8 - Pseudomonas has a plasmid containing the mer...Ch. 8 - Ciprofloxacin, erythromycin, and acyclovir are...Ch. 8 - HIV, the virus that causes AIDS, was isolated from...Ch. 8 - Human herpesvirus-8 (HHV-8) is common in parts of...
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- Sort the phrases into the appropriate bins depending on which protein they describe. 1) Binds at the replication fork 2) binds after the replication fork 3) binds ahead of the replication fork 4) breaks H-bonds between bases 5) prevents H-bonds between bases 6) breaks covalent bonds in DNA backbone Helicase: Topoisomerase: Single-strand binding protein:arrow_forwardPlace the following steps of DNA replication in order (from left to right) from the beginning to the end of the process. Reset Help priming of DNA synthesis with RNA primer removal of primers by DNA polymerase I exonuclease elongation of RNA primers by DNA polymerase III opening and stabilizing the DNA strands joining of Okazaki fragments by DNA ligase Beginning of replication End of replicationarrow_forwardMatch the enzymes involved in DNA replication with their function. Primase [ Choose ] [ Choose] Synthesizes short RNA segment to initiate new DNA strand Helicase Main enzyme that extends RNA primer by adding DNA nucleotides to it Stabilizes single-stranded DNA Relieves over-winding of DNA ahead of the replication fork Removes RNA primers preceding Okazaki fragment and replaces RNA nucleotides with DNA nucleotides Single-stranded binding proteins Unwinds DNA helix Synthesizes the ends of the linear chromosome Seals nicks between adjacent DNA segments DNA polymerase III [ Choose ] DNA polymerasel [ Choose ] DNA Ligase [ Choose ] Topoisomerase [ Choose ]arrow_forward
- Arrange the events of replication chronologically from A (first) to G (last event): -Sealing of phosphodiester linkage -Replacement of RNA primer with new DNA strand -Unwinding of the double helix -Addition of short RNA primers -Recognition of origin of replication -Synthesis of complementary DNA strand at the leading strand of the DNA -Excision of short RNA primersarrow_forwardSemi-discontinuous replication means that: DNA synthesis only occurs in the same direction as the replication fork. the double helix unwinds and each strand serves as a template for a new DNA molecule. there are Okazaki fragments associated with the leading strand. DNA synthesis occurs in both a 5’ to 3’ and a 3’ to 5’ direction. there is both a leading and a lagging strand associated with each replication fork.arrow_forwardCircle the following elements that are directly involved in DNA replication process DNA helicase RNA primase Sliding clamp DNA polymerase DNA ligasearrow_forward
- DNA polymerases are processive, which means that they remain tightly associated with the template strand while moving rapidly and adding nucleotides to the growing daughter stand. Which piece of the replication machinery accounts for this characteristic? Helicase Sliding Clamp Single Stranded Binding Protein Primasearrow_forwardMatch the following descriptions with the enzymes involved in DNA replication. 1. Adds an RNA primer to begin elongation 2. Removes the RNA primer from the beginning of the newly constructed strands 3. Splices lagging strand segments 4. Cleaves the rung of the DNA double helix ladder Description: DNA DNA Helicase Primase Enzyme: Polymerase Ligasearrow_forwardA region of DNA has six copies of a trinucleotide repeat. During one round of replication, the template strand slips as shown in the diagram. How many repeats will the DNA have if the newly synthesized strand is used as a template in the next round of replication? 1 5'-CAG 3'-GTC GTC 4 2 GTCH 3 2 CAG CAG GTC 3 GTC 5 4 CAG-3' GTC -5' 6 Next round of DNA replicationarrow_forward
- The figure below depicts various elements of the eukaryotic replication machinery in action. Enter the name for the protein depicted by each box. Box A Box B Box C Box D Box E Box F DNA polymerase on lagging strand (just finishing an Okazaki fragment) F Maintains polymerase association with DNA Enzyme extends separation of DNA strands Synthesizes RNA fragments that hybridize to DNA Relaxes supercoiled DNA ahead of replication fork Maintains DNA is single stranded state Promotes binding of processivity factors to DNA Newly synthesized strand pocoar Leading-strand template A New Okazaki fragment RNA primer E Lagging-strand template DNA polymerase on leading strand B C D Saaragon - Next Okazaki fragment will start here Parental DNA helixarrow_forwardTabulate the differences of Prokaryotic and Eukaryotic DNA replicationarrow_forwardInitiation of replication occurs at an "origin of replication" site that typically includes an AT-rich region. Initiation benefits from these AT-rich regions because: the AT-rich region recruits topoisomerase to begin the process of DNA replication. GC-rich regions are impossible to replicate because of their strong H-bonds. the AT-rich region recruits DNA polymerase to begin the process of DNA replication. adenine-thymine pairs are held together by two H-bonds, making them easier to separate during unwinding. None of the answers is correct.arrow_forward
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