Microbiology: An Introduction
12th Edition
ISBN: 9780321929150
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case
Publisher: PEARSON
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Textbook Question
Chapter 8, Problem 2A
Replication of the E. coli chromosome takes 40 to 45 minutes, but the organism has a generation time of 26 minutes. How does the cell have time to make complete chromosomes for each offspring cell?
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Which of the following is NOT correct concerning the initiation of replication in E. coli?
Question 29 options:
A) It involves a region of the DNA called oriC.
B) DnaA proteins bind to the DNA to begin separation of the strands.
C) The strands are initially separated at GC-rich regions of DNA.
D) Following initial separation, enzymes continue to separate the parental DNA strands around the rest of the chromosome.
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Chapter 8 Solutions
Microbiology: An Introduction
Ch. 8 - Briefly describe the components of DNA, and...Ch. 8 - DRAW IT Identify and mark each of the following on...Ch. 8 - Match the following examples of mutagens. Column A...Ch. 8 - The following is a code for a strand of DNA. a....Ch. 8 - Identify when (before transcription, after...Ch. 8 - Which sequence is the best target for damage by UV...Ch. 8 - You are provided with cultures with the following...Ch. 8 - Why are mutation and recombination important in...Ch. 8 - NAME IT Normally a commensal in the human...Ch. 8 - Match the following terms to the definitions in...
Ch. 8 - Match the following terms to the definitions in...Ch. 8 - Feedback inhibition differs from repression...Ch. 8 - Bacteria can acquire antibiotic resistance by all...Ch. 8 - Suppose you inoculate three flasks of minimal...Ch. 8 - Plasmids differ from transposons in that plasmids...Ch. 8 - Mechanism by which the presence of glucose...Ch. 8 - The mechanism by which lactose controls the lac...Ch. 8 - Two offspring cells are most likely to inherit...Ch. 8 - Which of the following is not a method of...Ch. 8 - Nucleoside analogs and ionizing radiation are used...Ch. 8 - Replication of the E. coli chromosome takes 40 to...Ch. 8 - Pseudomonas has a plasmid containing the mer...Ch. 8 - Ciprofloxacin, erythromycin, and acyclovir are...Ch. 8 - HIV, the virus that causes AIDS, was isolated from...Ch. 8 - Human herpesvirus-8 (HHV-8) is common in parts of...
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- How do the linear chromosomes in eukaryotes ensure that its ends are replicated completely?arrow_forwardHow does DNA replication occur in a precise manner to ensure that identical genetic information is put into the new chromatid? See Figures 8.12 and 8.13. FIGURE 8.12 In DNA replication, the two polynucleotide strands uncoil, and each is a template for synthesizing a new strand. A replicated DNA molecule contains one new strand and one old strand. This mechanism is called semiconservative replication. FIGURE 8.13 A close-up look at the process of DNA replication. (a) As the strands uncoil, bases are added to the newly synthesized strand by complementary base pairing with bases in the template strand. The new bases are linked together by DNA polymerase. (b) DNA synthesis can proceed only in the 5 3 direction; newly synthesized DNA on one template strand is made in short segments and linked together by the enzyme DNA ligase.arrow_forwardWhat happens to the ends of linear chromosomes in eukaryotes during replication? How are the ends of eukaryotic chromosomes maintained? What happens when the process goes wrong?arrow_forward
- When chromatin is treated with non-specific nucleases, what is the length of the resulting pieces of DNAarrow_forwardWhy is it important for a chromosome to be copied before mitosis?arrow_forwardSuppose that E. coli synthesizes DNA at a rate of 100,000 nucleotides per minute and takes 40 minutes to replicate its chromosome. (a) How many base pairs are present in the entire E. coli chromosome? (b) What is the physical length of the chromosome in its helical configuration—that is, what is the circumference of the chromosome if it were opened into a circle?arrow_forward
- Cell cultures can be synchronized, or brought into the same phase of the cell cycle, by various means. For example, adding thymidine to a cell culture causes all cells to become arrested early in S phase. What is the mechanism by which thymidine treatment blocks DNA replication?arrow_forwardAs shown, telomerase attaches additional DNA, six nucleotides at a time, to the ends of eukaryotic chromosomes. However, it makes only one DNA strand. Describe how the opposite strand is replicated.arrow_forwardA cell is produced with a mutation that causes it produce a completely defective TFIIH. What does this likely mean for the fate of the cell? A) The cell's copied DNA may contain more errors. B) the cell cannot make RNA C) Producing RNAs will be slower because the polymerase will fall off the DNA more often. D) The cell cannot replicate its DNAarrow_forward
- When a cell is to divide, its DNA must be replicated (copied). a) Indicate the main participating components and their functions. b) Why is it important that the replication goes right and that relatively few mutations occur during the replication?arrow_forwardThe amount of DNA in eukaryotic cells is significantly greater than in prokaryotes. With this in mind, how is the eukaryotic DNA replicated in a timely, synchronous fashion?arrow_forwardBelow is a diagram of DNA replication as currently believed to occur in E. coli. Arrows start from numbers and end at specific points. Answer the questions relating to the locations specified by the numbers (1) Which end (5' or 3') of the molecule is here? (2) Which enzyme is probably functioning here to deal with supercoils in the DNA? (3) Which enzyme is probably functioning here to unwind the DNA?arrow_forward
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