Microbiology: An Introduction
11th Edition
ISBN: 9780321733603
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case
Publisher: Benjamin Cummings
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Textbook Question
Chapter 8, Problem 6R
Identify when (before transcription, after transcription but before translation, after translation) each of the following regulatory mechanisms functions.
- a. ATP combines with an enzyme, altering its shape.
- b. A short RNA is synthesized that is complementary to mRNA.
- c. Methylation of DNA occurs.
- d. An inducer combines with a repressor.
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Arrange the statements in their proper order by writing the corresponding letter
(e.g. A) for each statement in the space provided below.
A. The single-stranded RNA would complement the target RNA.
B. Gene expression is inactivated once the mRNA is no longer accessible for
translation.
C. The risk-induced silencing complex which is composed of RNA and protein
subunits is formed.
D. Double-stranded, non-coding RNA is cleaved by Dicer.
E. The mRNA can be cleaved or remain bound by the RISC.
1.
2.
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Define and describe the roles of the following in transcription: a. transcription factors b. RNA polymerase c. promoter d. sigma factor e. enhancer f. TATA box
Define the following terms: a. promoter b. consensus sequence c. operon d. chromatin-remodeling complex e. general transcription factors
Chapter 8 Solutions
Microbiology: An Introduction
Ch. 8 - Briefly describe the components of DNA, and...Ch. 8 - DRAW IT Identify and mark each of the following on...Ch. 8 - Match the following examples of mutagens. Column A...Ch. 8 - The following is a code for a strand of DNA. a....Ch. 8 - Prob. 5RCh. 8 - Identify when (before transcription, after...Ch. 8 - Which sequence is the best target for damage by UV...Ch. 8 - You are provided with cultures with the following...Ch. 8 - Why are mutation and recombination important in...Ch. 8 - NAME IT Normally a commensal in the human...
Ch. 8 - Nucleoside analogs and ionizing radiation are used...Ch. 8 - Replication of the E. coli chromosome takes 40 to...Ch. 8 - Pseudomonas has a plasmid containing the mer...Ch. 8 - Match the following terms to the definitions in...Ch. 8 - Match the following terms to the definitions in...Ch. 8 - Feedback inhibition differs from repression...Ch. 8 - Bacteria can acquire antibiotic resistance by all...Ch. 8 - Suppose you inoculate three flasks of minimal...Ch. 8 - Plasmids differ from transposons in that plasmids...Ch. 8 - Mechanism by which the presence of glucose...Ch. 8 - The mechanism by which lactose controls the lac...Ch. 8 - Two offspring cells are most likely to inherit...Ch. 8 - Which of the following is not a method of...Ch. 8 - Ciprofloxacin, erythromycin, and acyclovir are...Ch. 8 - HIV, the virus that causes AIDS, was isolated from...Ch. 8 - Human herpesvirus-8 (HHV-8) is common in parts of...
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- Hydrogen bonds are important in DNA replication and transcription. They are relatively weak chemical bonds. Why is this a desirable feature for DNA? Describe the effect (s) of changing (mutating) the promoter on the transcription of the DNA strand/gene the promoter controls. What happens to protein synthesis if a nonsense codon is inserted into the gene? Explain why a point mutation does not necessarily change the original amino acid sequence. (Explain silent mutations) Choose any pentapeptide composed of five different amino acids. List the amino acids. Present one messenger RNA codon for each amino acids and the sequence of nucleotides on the DNA that originally coded for your pentapeptide.arrow_forwardGeneral transcription factors Select an answer and submit. For keyboard navigation, use the up/down arrow keys to select an answer. a a) act at every gene for a given RNA pol b b) act only at specific genes for under specific conditions c) must be very close to the promoter C d) none of the abovearrow_forwardIn eukaryotic cells, alternative splicing of the pre-mRNA (primary transcript) to form different mature mRNAs is an example of _________________ regulation. A) transcriptional B)post-transcriptional C) translational D) post-translationalarrow_forward
- he DNA site that is bound by a transcriptional activator regulates gene expression in a) cisb) transarrow_forwardWhat are two reasons that a eukaryotic gene (e.g. Green Fluorescent Protein in jellyfish) will not be expressed if it is inserted into a bacterial genome with no alterations? a. Bacterial genes do not contain introns b. Bacterial promoters have different consensus sequences than eukaryotic ones c. Transcription of this gene is affected by the simultaneous processes of transcription and translation d.arrow_forwardTranscriptional regulation often involves a regulatory protein that binds to a segment of DNA and a small effector molecule that binds to the regulatory protein. Do each of the following terms apply to a regulatory protein, a segment of DNA, or a small effector molecule? A. Repressor B. Inducer C. Operator site D. Corepressor E. Activator F. Attenuator G. Inhibitorarrow_forward
- Which of the following is not true regarding gene regulation that involves DNA bending? a. The precise distance between the regulatory sequence and the promoter is important. b. Effect can be to repress transcription c. Effect can be to activation transcription d. Regulated genes can be thousands of base pairs away from the regulatory sitesarrow_forwardWhich of the following best explains how the prokaryotic expression of a metabolic protein can be regulated when the protein is already present at a high concentration? a.Repressor proteins can be activated and bind to regulatory sequences to block transcription. b.Regulatory proteins can be inactivated to increase gene expression. c.Transcription factors can bind to regulatory sequences to increase RNA polymerase binding. d.Histone modification can prevent transcription of the gene.arrow_forwardWhich of the following is NOT TRUE about Eukaryotic Transcription: A. Occurs in the cytoplasm B. Pol II has 12 subunits C. Pol III transcribes tRNA genes D. It’s controlled by Cis-acting sequences E. Leads to specialization of cell functionarrow_forward
- If a cell's DNA was mutated such that it lost the promoter of a gene, you would expect: a. That ribosomes wouldn’t bind to that gene b. No effect since the promoter doesn't have any coding information for the amino acids in the polypeptide c. Ribosomes would never stop translating that gene d. Transcription factors for that gene would no longer bind to activate expression of that gene e. tRNA would no longer bind to the codons of that genearrow_forwardFor each statement about gene expression mechanisms, choose the correct end to the sentence. For each gene, the template strand for transcription is determined by…. The direction of translation is determined by…… The tissue-specificity of protein production is determined by…. choices: a. location of the start codon b. location of the promoter c. direction of polymerization by RNA polymerase d. none of these e. direction of movement of ribosomes f. overall orientation of the chromosomearrow_forwardA) List the steps for gene expression in prokaryotes and eukaryotes. B) Relate the differences in gene expression between prokaryotes and eukaryotes in gene expression regulation and explain what causes those differences.arrow_forward
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