Microbiology: An Introduction
11th Edition
ISBN: 9780321733603
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case
Publisher: Benjamin Cummings
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Textbook Question
Chapter 8, Problem 8MCQ
The mechanism by which lactose controls the lac operon.
Use the following choices to answer questions 7 and 8.
- a. catabolite repression
- b. DNA polymerase
- c. induction
- d. repression
- e. translation
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Students have asked these similar questions
Define the following terms: a. promoter b. consensus sequence c. operon d. chromatin-remodeling complex e. general transcription factors
Decide which operon each of the following characteristics applies to. Note: a description may apply
to both.
a. lac operon
b. trp operon
68. repressible
69. Turned off when end product is present (feedback inhibition)
70. Promoter, activator region, and 3 genes
71. Repressible
72. contain operator and promoter regions as well as transcript units of at least two or more coding
genes
73. Codes for catabolic enzymes that break down lactose
74. Inducible
75. Promoter, activator region, and 5 genes
76. Codes for anabolic enzymes that help manufacture tryptophan
77. Lactose is the co-inducer
78. Turned on when substrate is present
79. Tryptophan is the co-repressor
80. Regulatory gene segments
Imagine this scenario:
In E.coli the Operon X encodes for
Proteins Y, X and Z. Proteins Y, X and
Z are required for growth in the
presence of Drug A.
Outline the experiments that you
would conduct to assess whether
Protein B represses the transcription
of Operon X in the absence of Drug A.
Things to consider: 1. Transcription of
Operon X 2. Mutations 3. Chromatin
structure
Chapter 8 Solutions
Microbiology: An Introduction
Ch. 8 - Briefly describe the components of DNA, and...Ch. 8 - DRAW IT Identify and mark each of the following on...Ch. 8 - Match the following examples of mutagens. Column A...Ch. 8 - The following is a code for a strand of DNA. a....Ch. 8 - Prob. 5RCh. 8 - Identify when (before transcription, after...Ch. 8 - Which sequence is the best target for damage by UV...Ch. 8 - You are provided with cultures with the following...Ch. 8 - Why are mutation and recombination important in...Ch. 8 - NAME IT Normally a commensal in the human...
Ch. 8 - Nucleoside analogs and ionizing radiation are used...Ch. 8 - Replication of the E. coli chromosome takes 40 to...Ch. 8 - Pseudomonas has a plasmid containing the mer...Ch. 8 - Match the following terms to the definitions in...Ch. 8 - Match the following terms to the definitions in...Ch. 8 - Feedback inhibition differs from repression...Ch. 8 - Bacteria can acquire antibiotic resistance by all...Ch. 8 - Suppose you inoculate three flasks of minimal...Ch. 8 - Plasmids differ from transposons in that plasmids...Ch. 8 - Mechanism by which the presence of glucose...Ch. 8 - The mechanism by which lactose controls the lac...Ch. 8 - Two offspring cells are most likely to inherit...Ch. 8 - Which of the following is not a method of...Ch. 8 - Ciprofloxacin, erythromycin, and acyclovir are...Ch. 8 - HIV, the virus that causes AIDS, was isolated from...Ch. 8 - Human herpesvirus-8 (HHV-8) is common in parts of...
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- A mutation that inactivates the repressor gene of the lac operon results in (a) the continuous transcription of the structural genes (b) no transcription of the structural genes (c) the binding of the repressor to the operator (d) no production of RNA polymerase (e) no difference in the rate of transcriptionarrow_forwardWhich statement about the transcription attenuation mechanism is TRUE? 1.In some operons (e.g., the his operon), attenuation may be the only regulatory mechanism. 2. Sequences of the trp operon leader RNA resemble an operator. 3. The leader peptide acts by a mechanism that is similar to that of a repressor protein. 4.The leader peptide gene of the trp operon includes no Trp codons. 5. The leader peptide is an enzyme that catalyzes transcription attenuation.arrow_forwardBelow is an electron micrograph illustrating the process of simultaneous transcription and translation in a prokaryotic cell. Use this illustration to answer the question. A. Which of the following statements is correct? 1. The direction indicated by A, is the correct direction of RNA polymerase movement. 2. If C and D are both pointing to the first ribosome in the chain, then ribosome C is carrying a longer polypeptide chain. 3. If E is pointing to mRNA, then it is pointing to the 5'end of the mRNA. 4. If B is pointing to the DNA, then it is pointing to the 5' end of the DNA. O A. 1, 2 and 3 O B. 1 and 3 O C. 2 and4 O D. 4 only O E. All of 1, 2, 3 and 4 are correctarrow_forward
- Can you please answer number 10 and all of the sub partsarrow_forwardSome antibiotic drugs fight infection by interfering with DNA replication, transcription, or translation in bacteria. Indicate whether each of the following antibiotic drug effects is on replication, transcription, or translation. HINT Each answer (replication, transcription, and translation) is used only once for the following: a. Rifampin binds to bacterial RNA polymerase. b. Streptomycin binds bacterial ribosomes, disabling them. c. Quinolone blocks an enzyme that prevents bacterial DNA from unwinding.arrow_forwardIn the trp operon, tryptophan is best characterized as being a: A. repressor B. inducer C. corepressor D. DNA-binding domainarrow_forward
- Describe the following a. Core enzyme RNA polymerase b. Sigma factor c. Promoter consensus sequence d. Rho Dependent Factor e. Intrinsic Factor f. Abortive Initiationarrow_forwardAnswer this 3 part question: (MC) What is the function of lactose in regulation of the lac operon? 1. activates a repressor protein 2. activates an activator protein 3. inactivates a repressor protein 4. inactivates an activator protein Consider a bacterial strain with a lac operon Р. О Y A a) Mention which parts of the DNA region shown in the diagram encode for • Enzyme proteins? • Operator • Promoter • Repressor gene b) Where would the lac repressor be bound in a E. coli cell that is growing in ( • high lactose? No lactosearrow_forwardWhich of these BEST DESCRIBE tryptophan in the Trp Operon? A. Acts as a corepressor B. Acts as a coactivator C. Acts as an inducer D. Acts as an enhancerarrow_forward
- Suppose that the E. coli gene for the lac operon repressor has a mutation that makes the repressor ineffective. What effect would this have on the bacterium’s ability to catabolize lactose? Would the mutant strain have an advantage over wild-type cells? Explain your answerarrow_forwardWhich of the following statements is true regarding transcriptional attenuation of the trp operon? a. The leader peptide encodes a protein that binds to the operator site b. Translation of the leader peptide stalls when tryptophan levels are high c. Stalling of the ribosome in region 1 of the leader peptide inhibits formation of the transcriptional terminator sequence d. This kind of mechanism can only occur in prokaryotes e. Both c. and d. are truearrow_forwardExplain how the following conditions will affect the regulation of the operon. (3 pts) Low cAMP levels; E. coli grown in a high lactose mediumarrow_forward
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