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The diagram below shows a DNA duplex. The template strand is identified, as is the location of the
Assume this region contains a gene transcribed in a bacterium. Identify the location of promoter consensus sequences and of the transcription termination sequence.
Assume this region contains a gene transcribed to form mRNA in a eukaryote. Identify the location of the most common promoter consensus sequences.
If this region is a eukaryotic gene transcribed by RNA polymerase III, where are the promoter consensus sequences located?
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Chapter 8 Solutions
GENETIC ANALYSIS: AN INTEG. APP. W/MAS
- DNA Replication Terms _____DNA polymerase III _____Primase _____Helicase _____Lagging Strand _____Leading Strand _____RNA Primer _____Single Strand Binding Protein _____5’ _____3’ _____DNA Ligase _____ Topoisomerase _____Template Strand _____Coding Strandarrow_forwardUse the gel to answer the following questions. You will be constructing a map of the plasmid, pDiddy. There are NO sites where multiple enzymes cut. How many base pairs long is the plasmid? 3kb 2kb 4kb 5kbarrow_forwardThe image shows a replication fork with template DNA strands, new DNA strands, and some replication proteins. Label the mage by moving the terms or descriptive phrases to the appropriate targets. lagging strand (or Okazaki fragment) Answer Bank protein that synthesizes RNA primer DnaB helicase DNA gyrase (topoisomerase)arrow_forward
- Indicate the proteins involved in the following steps of DNA replication in E. coli a. ___________ initiator of replication by binding to the origin of replication of the prokaryote ___________ a dimer that unzips the DNA helix ___________ an enzyme responsible for relieving positive supercoils ahead of replication fork ___________ maintains DNA in single-stranded state ___________ primary replicating enzyme ___________ synthesizes RNA primers The resulting gap by removal of RNA primer is filled in by ___________. h. the resulting nick is sealed by _____________.arrow_forwardCircle the section on the DNA template where the example primer would bind.arrow_forwardplease dont give hand writting solution pleasearrow_forward
- Shown is a sequencing gel. Read the sequence off the gel and determine the first 4 nucleotides (or bases) based on the fact replication occurs 5¹ to 3¹. A I G C Answer:arrow_forwardDetermine the characteristics of this piece of double-stranded DNA. GTCTTTTACTTGAAT CAGAAAATGAACTTA Phosphodiester bonds: _________________ Pyrimidines: _________________ Hydrogen bonds: _________________ Nitrogenous bases: _________________ A/T Ratio: _________________arrow_forwardInduce a silent mutation in the template strand. Illustrate the output.arrow_forward
- The image shows a replication fork, template DNA strands, and new DNA strands. Label the image. and 13' 15' 3' 5' Answer Bank lagging strand leading strand replication fork template DNA strandsarrow_forwardThe bacterial primase enzyme synthesizes _______________________. a short DNA primer using RNA as a template a short protein primer using RNA as a template an RNA strand in absence of template DNA a short DNA primer using DNA as a template a short RNA primer using DNA as a templatearrow_forwardThe chromatogram shows fluorescent peak data from a dye-terminating nucleotide-sequencing reaction. The peaks are shown with shortest fragment on the left to longer fragments on the right. T •C A Select the DNA sequence that matches the data. 5-ТАТAСТТАСGAAGT-3' 5'-GTCCTACGGACGCG–3' 5'-ATATGAATGCTTCA–3' 5'-TGAAGCATTCATAT–3' 5-АСТТCGTAAGTATA-3'arrow_forward
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