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Concept explainers
To analyze:
The complete sequence of a gene is present in a
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a. Sketch the relative positions expected for the DNA fragments in this gel electrophoresis analysis.
b. Identify the relative positions of bands in lane
c. The relative positions of bands in lanes
Introduction:
Band shift assay is a technique to study the interactions between protein and DNA. The assay is based on non-denaturing gel electrophoresis that runs complexes of proteins and DNA through it. The migration of either DNA or RNA is compared to the migration of a complex of particular protein with DNA or RNA. This study helps to reveal the degree and nature of binding between them. It is also termed as electrophoretic mobility shift assay. The Band shift assay is the separation technique for proteins, DNA and RNA, the mixture of interest is loaded onto an agarose gel and observed for speed, size, and shape.
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Chapter 8 Solutions
GENETIC ANALYSIS: AN INTEG. APP. W/MAS
- Describe the banding pattern seen in the gel. What does that tell you about the role of annealing temperature in gene amplification?arrow_forwardYou are attempting to prepare a single gene knockout library using the pRL27 transposon system. You grow the donor E. coli in Luria broth containing both kanamycin and diaminopimelic acid (DAP) and your recipient Serratia rubidaea in plain Luria broth. You combine an equal ratio of donor and recipient cultures and plate the mixture onto Luria agar supplemented with DAP. After 24 hours incubation at 37°C, you create a cell slurry and plate the cells onto Luria agar aupplemented with kanamycin. After 24 hours incubation at 37°C, you find that no colonies grow. What best explains this outcome? A. Failure to supplement media with DAP B. Failure to remove antiobiotic containing media C. Failure to incubate for a sufficient length of time D. Failure to incubate at the appropiate temperature E. Failure to use the proper mating mix ratioarrow_forwardA series of exonuclease deletions were used to study the promoter of the rice hemA gene, giving the results shown below (the intact promoter is on top). Based on these results, what conclusions could you draw from each of the deletion construct, and what do you know about the nature of the promoter? Relative activity -500 Reporter gene 100% Reporter gene 180% -350 -250- Reporter gene 180% 75% -150 Reporter gene 45% Reporter gene -100 0% - 8 Reporter genearrow_forward
- How many nucleotides are in the consensus coding sequence (CDS) of the KMT2D transcript? answer in numerical digits only.arrow_forwardTranscriptome analysis involves two separate methodologies: gene expression and RNA seq analyses. The 10 items below are a scrambled listing of the steps used in the two procedures. Identify the steps involved in RNA seq from the list below. Use the numbers in the list to refer to each step. Once the steps for RNA seq have been identified, write the steps in the order in which they are performed during the experiment. (1) DNA sequencing (2) Allow for hybridization and wash excess cRNA. (3) Mix labeled cRNA with array chip. (4) PCR amplification (5) Measure fluorescence intensity to determine abundance of transcripts. (6) Add labeled cRNA at each microarray location. (7) Map cDNA sequences to the genome of the organism to determine identity and abundance of transcripts. (8) mRNA isolation from cells (9) Prepare fluorescently labeled cRNA probes (10) cDNA synthesisarrow_forwardTo identify the following types of genetic occurrences, would acomputer program use sequence recognition, pattern recognition,or both?A. Whether a segment of Drosophila DNA contains a P element(which is a specific type of transposable element)B. Whether a segment of DNA contains a stop codonC. In a comparison of two DNA segments, whether there is aninversion in one segment compared with the other segmentD. Whether a long segment of bacterial DNA contains one ormore genesarrow_forward
- A. If the image below is an electron micrograph showing the result of an experiment in which DNA and RNA were boiled and hybridized together, which of the strands is the template for transcription? B.If the image below is an electron micrograph showing the result of an experiment in which DNA and RNA were boiled and hybridized together, which of the strands is the RNA?arrow_forwardBriefly explain how synthetic probes are created to screen a DNA library when the protein encoded by the gene is known.arrow_forwardPlease make this as SIMPLE as possible Question: regarding to horizontal gene transfer please only discuss what cells are still alive or dead between transformation, trasnduction, and conjugation Be specific when discussing the donor versus recipient cell, and if the donor and recipient cells are still alive after each horizontal gene transfer event is completearrow_forward
- In the Ames test shown in Figure 16-17, what is the reason for adding the liver extract to each sample?arrow_forwardA rice MADS box transcription factor gene contains a number of sequences that are removed in the processing of the mRNA transcript. Bacterial cells cannot excise these sequences from mRNA transcripts, yet via recombinant DNA technology, this gene can be cloned into a bacterial cell and produce the MADS box factor protein. Explain how this is possible.arrow_forwardA new technique for rapidly determining the nuclesome structure for regions of chromatin is called Mnase-Seq. Briefly, in MNase-Seq experiments, the chromatin is digested with Micrococcal Nuclease (MNase) and then the resulting digest is subjecting to high throughput sequencing to identify sequences digested by enzyme. See this short article for complete explanation. In the experiment below, researchers conducted an Mnase Seq experiment on a region of a chromosome in the absence (OHT-) and presence (OHT+) of a Protein X. This protein has a dramatic effect on the expression of the Igll1 gene, but no effect on Top3b nor Vpreb1 genes. Based on this information, explain what Protein X is doing to influence the Igll1 gene (limit 4-5 sentences).arrow_forward
- Human Heredity: Principles and Issues (MindTap Co...BiologyISBN:9781305251052Author:Michael CummingsPublisher:Cengage Learning
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