(a) Interpretation: The reason corresponding to the decrease in the amount of active enzyme with time of incubation is to be stated. Concept introduction: Enzymes being the catalysts increase the rate of reaction . In an enzyme-catalyzed reaction, the rate of reaction for the product formation depends on the concentration of enzyme as well as the substrate involved in the reaction via formation of an enzyme substrate complex.

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Answer 1

Question

Chapter 8, Problem 41P

Interpretation Introduction

(a)

Interpretation:

The reason corresponding to the decrease in the amount of active enzyme with time of incubation is to be stated.

Concept introduction:

Enzymes being the catalysts increase the rate of reaction. In an enzyme-catalyzed reaction, the rate of reaction for the product formation depends on the concentration of enzyme as well as the substrate involved in the reaction via formation of an enzyme substrate complex.

Interpretation Introduction

(b)

Interpretation:

The reason corresponding to the declination in the amount of enzyme from the PLP-deficient cells more rapidly needs to be explained.

Concept introduction:

Enzymes being the catalysts increase the rate of reaction. In an enzyme-catalyzed reaction, the rate of reaction for the product formation depends on the concentration of enzyme as well as the substrate involved in the reaction via formation of an enzyme substrate complex.

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You’ve isolated a protein and determined that the Native molecular weight of the holoenzyme is 160 kD using size exclusion chromatography. Analysis of this protein using SDS-PAGE revealed 2 bands, one at 100 kD and one at 30 kD. The enzyme was found to be 0.829% NAD (by weight). What further can be said regarding the structure of the polypeptide?

Answer 2

Question

Chapter 8, Problem 41P

Interpretation Introduction

(a)

Interpretation:

The reason corresponding to the decrease in the amount of active enzyme with time of incubation is to be stated.

Concept introduction:

Enzymes being the catalysts increase the rate of reaction. In an enzyme-catalyzed reaction, the rate of reaction for the product formation depends on the concentration of enzyme as well as the substrate involved in the reaction via formation of an enzyme substrate complex.

Interpretation Introduction

(b)

Interpretation:

The reason corresponding to the declination in the amount of enzyme from the PLP-deficient cells more rapidly needs to be explained.

Concept introduction:

Enzymes being the catalysts increase the rate of reaction. In an enzyme-catalyzed reaction, the rate of reaction for the product formation depends on the concentration of enzyme as well as the substrate involved in the reaction via formation of an enzyme substrate complex.

Expert Solution & Answer

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Answer 3

Question

Chapter 8, Problem 41P

Interpretation Introduction

(a)

Interpretation:

The reason corresponding to the decrease in the amount of active enzyme with time of incubation is to be stated.

Concept introduction:

Enzymes being the catalysts increase the rate of reaction. In an enzyme-catalyzed reaction, the rate of reaction for the product formation depends on the concentration of enzyme as well as the substrate involved in the reaction via formation of an enzyme substrate complex.

Interpretation Introduction

(b)

Interpretation:

The reason corresponding to the declination in the amount of enzyme from the PLP-deficient cells more rapidly needs to be explained.

Concept introduction:

Enzymes being the catalysts increase the rate of reaction. In an enzyme-catalyzed reaction, the rate of reaction for the product formation depends on the concentration of enzyme as well as the substrate involved in the reaction via formation of an enzyme substrate complex.

Expert Solution & Answer

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See solution

Answer 4

Question

Chapter 8, Problem 41P

Interpretation Introduction

(a)

Interpretation:

The reason corresponding to the decrease in the amount of active enzyme with time of incubation is to be stated.

Concept introduction:

Enzymes being the catalysts increase the rate of reaction. In an enzyme-catalyzed reaction, the rate of reaction for the product formation depends on the concentration of enzyme as well as the substrate involved in the reaction via formation of an enzyme substrate complex.

Interpretation Introduction

(b)

Interpretation:

The reason corresponding to the declination in the amount of enzyme from the PLP-deficient cells more rapidly needs to be explained.

Concept introduction:

Enzymes being the catalysts increase the rate of reaction. In an enzyme-catalyzed reaction, the rate of reaction for the product formation depends on the concentration of enzyme as well as the substrate involved in the reaction via formation of an enzyme substrate complex.

Expert Solution & Answer

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Answer 5

Chapter 8, Problem 41P

Interpretation Introduction

(a)

Interpretation:

The reason corresponding to the decrease in the amount of active enzyme with time of incubation is to be stated.

Concept introduction:

Enzymes being the catalysts increase the rate of reaction. In an enzyme-catalyzed reaction, the rate of reaction for the product formation depends on the concentration of enzyme as well as the substrate involved in the reaction via formation of an enzyme substrate complex.

Interpretation Introduction

(b)

Interpretation:

The reason corresponding to the declination in the amount of enzyme from the PLP-deficient cells more rapidly needs to be explained.

Concept introduction:

Enzymes being the catalysts increase the rate of reaction. In an enzyme-catalyzed reaction, the rate of reaction for the product formation depends on the concentration of enzyme as well as the substrate involved in the reaction via formation of an enzyme substrate complex.

Answer 6

Chapter 8, Problem 41P

Interpretation Introduction

(a)

Interpretation:

The reason corresponding to the decrease in the amount of active enzyme with time of incubation is to be stated.

Concept introduction:

Enzymes being the catalysts increase the rate of reaction. In an enzyme-catalyzed reaction, the rate of reaction for the product formation depends on the concentration of enzyme as well as the substrate involved in the reaction via formation of an enzyme substrate complex.

Answer 7

Chapter 8, Problem 41P

Interpretation Introduction

(a)

Interpretation:

The reason corresponding to the decrease in the amount of active enzyme with time of incubation is to be stated.

Concept introduction:

Enzymes being the catalysts increase the rate of reaction. In an enzyme-catalyzed reaction, the rate of reaction for the product formation depends on the concentration of enzyme as well as the substrate involved in the reaction via formation of an enzyme substrate complex.

Answer 8

Interpretation Introduction

(b)

Interpretation:

The reason corresponding to the declination in the amount of enzyme from the PLP-deficient cells more rapidly needs to be explained.

Concept introduction:

Enzymes being the catalysts increase the rate of reaction. In an enzyme-catalyzed reaction, the rate of reaction for the product formation depends on the concentration of enzyme as well as the substrate involved in the reaction via formation of an enzyme substrate complex.

Answer 9

Interpretation Introduction

(b)

Interpretation:

The reason corresponding to the declination in the amount of enzyme from the PLP-deficient cells more rapidly needs to be explained.

Concept introduction:

Enzymes being the catalysts increase the rate of reaction. In an enzyme-catalyzed reaction, the rate of reaction for the product formation depends on the concentration of enzyme as well as the substrate involved in the reaction via formation of an enzyme substrate complex.

Answer 10

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Answer 11

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Answer 12

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Answer 13

Ch. 8 - Prob. 1P Ch. 8 - Prob. 2P Ch. 8 - Prob. 3P Ch. 8 - Prob. 4P Ch. 8 - Prob. 5P Ch. 8 - Prob. 6P Ch. 8 - Prob. 7P Ch. 8 - Prob. 8P Ch. 8 - Prob. 9P Ch. 8 - Prob. 10P

Solution Summary: The author explains that enzymes increase the rate of reaction, and the amount of enzyme from PLP-deficient cells decreases with time of incubation.

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