Biology: The Unity and Diversity of Life (MindTap Course List)
14th Edition
ISBN: 9781305073951
Author: Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr
Publisher: Cengage Learning
expand_more
expand_more
format_list_bulleted
Concept explainers
Videos
Textbook Question
Chapter 8, Problem 3DAA
Hershey–Chase Experiments The graph shown in FIGURE 8.5 is reproduced from an original 1952 publication by Hershey and Chase. Bacteriophage were labeled with radioactive tracers and allowed 10 infect bacteria. The virus–bacteria mixtures were then whirled in a blender to dislodge any viral components attached to the exterior of the bacteria. Afterward, radioactivity from the tracers was measured.
FIGURE 8.5 Detail of Alfred Hershey and Martha Chase’s 1952 publication describing their experiments with bacteriophage.
“Infected bacteria” refers to the percentage of bacteria that survived the blender.
How did the researchers know that the radioisotopes in the fluid came from outside of the bacterial cells and not from bacteria that had been broken apart by whirling in the blender?
Expert Solution & Answer
Trending nowThis is a popular solution!
Students have asked these similar questions
Figure 5: MCF-7 cells were incubated for 24 h with the drug dilution buffer (control) or various concentrations of indibulin. They were then stained with an antibody against α-tubulin (red channel), and Hoechst 33258 (blue channel). Scale bar = 10 μm.
Question : Comment Figure 5
Give detailed Solution with explanation needed (don't copy the answer other site
The following image is of an agarose gel. If DNA samples were loaded to this gel and the electrophoresis experiment was started, explain what would happen and why.
Chapter 8 Solutions
Biology: The Unity and Diversity of Life (MindTap Course List)
Ch. 8 - Which is not a nucleotide base in DNA? a. adenine...Ch. 8 - What are the base-pairing rules for DNA? a. A-G,...Ch. 8 - Variation in _____ is the basis of variation in...Ch. 8 - One species' DNA differs from others in its...Ch. 8 - Prob. 5SQCh. 8 - Prob. 6SQCh. 8 - Prob. 7SQCh. 8 - When DNA replication begins, ______. a. the two...Ch. 8 - DNA replication requires _______. a. DNA...Ch. 8 - HersheyChase Experiments The graph shown in FIGURE...
Ch. 8 - HersheyChase Experiments The graph shown in FIGURE...Ch. 8 - HersheyChase Experiments The graph shown in FIGURE...Ch. 8 - HersheyChase Experiments The graph shown in FIGURE...Ch. 8 - HersheyChase Experiments The graph shown in FIGURE...Ch. 8 - Energy that drives the attachment of a nucleotide...Ch. 8 - The phrase "5 prime to 3 prime" refers to the...Ch. 8 - After DNA replication, a eukaryotic chromosome...Ch. 8 - Prob. 13SQCh. 8 - Prob. 14SQCh. 8 - Prob. 15SQCh. 8 - Prob. 1CTCh. 8 - Woolly mammoths have been extinct for about 4,000...Ch. 8 - Xeroderma pigmentosum is an inherited disorder...
Additional Science Textbook Solutions
Find more solutions based on key concepts
An obese 55-year-old woman consults her physician about minor chest pains during exercise. Explain the physicia...
Biology: Life on Earth with Physiology (11th Edition)
Why do scientists think that all forms of life on earth have a common origin?
Genetics: From Genes to Genomes
Gregor Mendel never saw a gene, yet he concluded that some inherited factors were responsible for the patterns ...
Campbell Essential Biology (7th Edition)
To test your knowledge, discuss the following topics with a study partner or in writing ideally from memory. Th...
HUMAN ANATOMY
Choose the best answer to each of the following. Explain your reasoning. If Earth were twice as far as it actua...
Cosmic Perspective Fundamentals
What is the pH range for acidic solutions? For basic solutions?
EBK INTRODUCTION TO CHEMISTRY
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- TRY TO KEEP IN SHORT AND USE OWN WORD FOR THIS QUESTION You are studying a type of bacteria isolated from the acidic water runoff of a mining operation. You subject two batches of the same bacteria type to different environmental growth conditions. One batch is grown at pH 2, while the other is grown at pH 7. All other environmental parameters are kept identical between the two batches. You then collect their proteins and run a Western blot using an antibody that binds to a proton efflux pump protein (which actively expends energy to pump protons out of a cell). How would you characterize the information obtained in this experiment? What does it tell you, and why is that potentially valuable information?arrow_forwardExperiment: DNA Extraction from Banana The procedures are attached below. Questions: 1. What does mashing do to the fruit? And why is it needed to add detergents?2. What do you think the ethanol does? Why can’t we use room temperature ethanol?arrow_forwarddid i identify sample a correctly? or is it actually none?arrow_forward
- Transcribed Image Text:Complete the following tasks. You discovered that a species of bacteria can break down StyrofoamT (polystyrene) products due to an enzyme it produces, polystyrenase. You wish to study the gene that codes for this enzyme. Task 1: DNA Extraction To begin work on the bacterium, you begin by extracting its genomic DNA (GDNA). What is the purpose of the following procedures? Answer briefly but completely. Using sodium dodecyl sulfate, a detergent Answer: а. b. Adding RNase A and Proteinase K during extraction Answer: c. Adding ethanol before recovering the DNA extract С. Answer: Task 2: Polymerase Chain Reaction After purifying the gDNA extract, you want to isolate and amplify the polystyrenase gene. You perform PCR using the appropriate gene-targeted primers. What is the purpose of the following PCR components? Answer briefly but completely. DNA polymerase isolated from Thermus aquaticus Answer: а. b. Deoxynucleotide triphosphates (DNTPS) Answer: С. Forward and…arrow_forwardProcedure: 1. Using the DNA provided transcribe DNA into mRNA. 2. Use the mRNA strand you created and break it up into codons. 3. Plug the codons into the amino acid chart to determine the correct amino acid needed to build that protein. 4. Identify the protein you made by comparing the sequence to the pictures 5. Answer the questions for each protein molecule you build before moving on to the next. Protein 1: DNA A AGACCGTATAC mRNA Amino Acid Sequence 1. Which kind of protein molecule did this gene make? 2 How does this protein help the body maintain homeostasis2arrow_forwardwhat are the volume dilution and dilution factor for bacteriophage A，B，Carrow_forward
- Which plate could represent the results of the transformation experiment positive control plated on LB if the transformation procedure was too harsh on the bacterial cells? Refer to the letters on top of the image. Ignore the letters at the bottom. B A O A OB O none of the choices are correctarrow_forwardquestion: Can you summarize and explain for me what you want to tell in the article below? When I read it myself, I do not understand exactly what is meant by the article. It would be nice if you could highlight the important points. You can use them in a figure or diagram to explain. thank you and hava a nice day :) Article: Biomimetic Engineering of Nanodelivery Systems: Artificial Viruses in the Making In an effort to engineer the next generation of nanoscale vectors, scientists have moved from using inorganic components aimed at obtaining inert structures to utilizing biological building blocks that are able to convey additional functionalities to the resulting construct. To cope with the complexity of the body and to evade the multiple layers of defense that tissues and organs have, it is critical to rely on the ability of certain materials to interact with, rather than to eschew, the biology of our body. Every NP system conceived to date faces one common fate: whether injected,…arrow_forwardDNA Extraction by Alkaline Lysis Procedure: 1. Spin 1.5 ml of cells in a microcentrifuge at maximum speed (12,000 rpm) for 20s to pellet. Remove the supernatant completely with a Pasteur pipet or a plastic pipettor tip. The spins can be performed at 4C or at room temperature. Longer spins make it difficult to resuspend cells. 2. Resuspend pellet in 100µl GTE solution and let sit 5 min at room temperature. Be sure cells are completely resuspended. 3. Add 200µl NaOH/SDS solution, mix by tapping tube with finger, and place on ice for 5 min. 4. Add 150µl potassium acetate solution and vortex at maximum speed for 2s to mix. Place on ice for 5-15 min. Be sure mixing is complete. 5. Spin 3 min at 12,000 rpm to pellet cell debris and chromosomal DNA. 6. Transfer 0.4 ml supernatant to a fresh tube, mix it with 0.8 ml of 95% ethanol or 0.4 ml isopropanol, and let sit 2 min at room temperature precipitate nucleic acids. 7. Spin at 12,000rpm for 3 min at room temperature to pellet plasmid DNA and…arrow_forward
- Below are the events that occur when recombinant DNA procedure is performed. Identify and describe each process. Name enzyme X _____________ Outline process B Outline the process which takes place inside the bacterium.arrow_forwardThe table below summarises the three stages of Meselson and Stahl's experiment and their results. (a) Complete the table by drawing, in the appropriate boxes, diagrams of the DNA molecules and mark the position and size of the DNA bands in the tubes. Experimental stage Diagram to show the strands in the DNA Position and size of DNA bands in the tube of molecules of the bacteria separating solution Stage 1 Bacteria grown for several generations in culture medium containing heavy nitrogen Stage 2 The bacteria from the end of stage 1 were grown for another generation in culture medium containing light nitrogen TA2G - Completed forms must be available for Open Awards extermal moderation purposes. Page 7 of 13 Stage 3 The bacteria from the end of stage 2 were grown for one more generation in culture medium containing light nitrogen (b) The bacteria at the end of stage two were grown for five more generations. After each generation, what would you expect to see in the test tube? Draw these…arrow_forwardplease help with calculationarrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
Biology: The Unity and Diversity of Life (MindTap...BiologyISBN:9781305073951Author:Cecie Starr, Ralph Taggart, Christine Evers, Lisa StarrPublisher:Cengage Learning
Biology: The Unity and Diversity of Life (MindTap...
Biology
ISBN:9781305073951
Author:Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr
Publisher:Cengage Learning
What Is A Virus ? ; Author: Peekaboo Kidz;https://www.youtube.com/watch?v=YS7vsBgWszI;License: Standard YouTube License, CC-BY