(a) Interpretation: Whether the given reaction proceeds as written or not is to be stated. The final concentrations of glucose − 6 − phosphate and glucose − 1 − phosphate is to be stated. Concept introduction: When any reaction is at equilibrium then a constant expresses a relationship between the reactant side and the product side. This constant is known as equilibrium constant. It is denoted by K eq . The equilibrium constant is independent of the initial amount of the reactant and product.

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Answer 1

Question

Chapter 8, Problem 19P

Interpretation Introduction

(a)

Interpretation:

Whether the given reaction proceeds as written or not is to be stated. The final concentrations of glucose−6−phosphate and glucose−1−phosphate is to be stated.

Concept introduction:

When any reaction is at equilibrium then a constant expresses a relationship between the reactant side and the product side. This constant is known as equilibrium constant. It is denoted by Keq. The equilibrium constant is independent of the initial amount of the reactant and product.

Interpretation Introduction

(b)

Interpretation:

The cellular condition under which glucose-1-phosphate is produced at a high rate is to be stated.

Concept introduction:

When any reaction is at equilibrium then a constant expresses a relationship between the reactant side and the product side. This constant is known as equilibrium constant. It is denoted by Keq. The equilibrium constant is independent of the initial amount of the reactant and product.

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The beta-lactamase hydrolyzes the lactam-ring in penicillin. Describe the mechanism of hydrolysis, insuring to include the involvement of S, D, & K in the reaction sequence. Please help

To map the active site of beta-lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine. Why doesn't D in this hexapeptide not participate in the hydrolysis of the beta-lactam ring even though S, K, and D are involved in the catalyst?

To map the active site of -lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine. Using the experimental results described above derive the primary sequence of the active site hexapeptide. Please help!

Answer 2

Question

Chapter 8, Problem 19P

Interpretation Introduction

(a)

Interpretation:

Whether the given reaction proceeds as written or not is to be stated. The final concentrations of glucose−6−phosphate and glucose−1−phosphate is to be stated.

Concept introduction:

When any reaction is at equilibrium then a constant expresses a relationship between the reactant side and the product side. This constant is known as equilibrium constant. It is denoted by Keq. The equilibrium constant is independent of the initial amount of the reactant and product.

Interpretation Introduction

(b)

Interpretation:

The cellular condition under which glucose-1-phosphate is produced at a high rate is to be stated.

Concept introduction:

When any reaction is at equilibrium then a constant expresses a relationship between the reactant side and the product side. This constant is known as equilibrium constant. It is denoted by Keq. The equilibrium constant is independent of the initial amount of the reactant and product.

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Answer 3

Question

Chapter 8, Problem 19P

Interpretation Introduction

(a)

Interpretation:

Whether the given reaction proceeds as written or not is to be stated. The final concentrations of glucose−6−phosphate and glucose−1−phosphate is to be stated.

Concept introduction:

When any reaction is at equilibrium then a constant expresses a relationship between the reactant side and the product side. This constant is known as equilibrium constant. It is denoted by Keq. The equilibrium constant is independent of the initial amount of the reactant and product.

Interpretation Introduction

(b)

Interpretation:

The cellular condition under which glucose-1-phosphate is produced at a high rate is to be stated.

Concept introduction:

When any reaction is at equilibrium then a constant expresses a relationship between the reactant side and the product side. This constant is known as equilibrium constant. It is denoted by Keq. The equilibrium constant is independent of the initial amount of the reactant and product.

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Answer 4

Question

Chapter 8, Problem 19P

Interpretation Introduction

(a)

Interpretation:

Whether the given reaction proceeds as written or not is to be stated. The final concentrations of glucose−6−phosphate and glucose−1−phosphate is to be stated.

Concept introduction:

When any reaction is at equilibrium then a constant expresses a relationship between the reactant side and the product side. This constant is known as equilibrium constant. It is denoted by Keq. The equilibrium constant is independent of the initial amount of the reactant and product.

Interpretation Introduction

(b)

Interpretation:

The cellular condition under which glucose-1-phosphate is produced at a high rate is to be stated.

Concept introduction:

When any reaction is at equilibrium then a constant expresses a relationship between the reactant side and the product side. This constant is known as equilibrium constant. It is denoted by Keq. The equilibrium constant is independent of the initial amount of the reactant and product.

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Answer 5

Chapter 8, Problem 19P

Interpretation Introduction

(a)

Interpretation:

Whether the given reaction proceeds as written or not is to be stated. The final concentrations of glucose−6−phosphate and glucose−1−phosphate is to be stated.

Concept introduction:

When any reaction is at equilibrium then a constant expresses a relationship between the reactant side and the product side. This constant is known as equilibrium constant. It is denoted by Keq. The equilibrium constant is independent of the initial amount of the reactant and product.

Interpretation Introduction

(b)

Interpretation:

The cellular condition under which glucose-1-phosphate is produced at a high rate is to be stated.

Concept introduction:

When any reaction is at equilibrium then a constant expresses a relationship between the reactant side and the product side. This constant is known as equilibrium constant. It is denoted by Keq. The equilibrium constant is independent of the initial amount of the reactant and product.

Answer 6

Chapter 8, Problem 19P

Interpretation Introduction

(a)

Interpretation:

Whether the given reaction proceeds as written or not is to be stated. The final concentrations of glucose−6−phosphate and glucose−1−phosphate is to be stated.

Concept introduction:

When any reaction is at equilibrium then a constant expresses a relationship between the reactant side and the product side. This constant is known as equilibrium constant. It is denoted by Keq. The equilibrium constant is independent of the initial amount of the reactant and product.

Answer 7

Chapter 8, Problem 19P

Interpretation Introduction

(a)

Interpretation:

Whether the given reaction proceeds as written or not is to be stated. The final concentrations of glucose−6−phosphate and glucose−1−phosphate is to be stated.

Concept introduction:

When any reaction is at equilibrium then a constant expresses a relationship between the reactant side and the product side. This constant is known as equilibrium constant. It is denoted by Keq. The equilibrium constant is independent of the initial amount of the reactant and product.

Answer 8

Interpretation Introduction

(b)

Interpretation:

The cellular condition under which glucose-1-phosphate is produced at a high rate is to be stated.

Concept introduction:

When any reaction is at equilibrium then a constant expresses a relationship between the reactant side and the product side. This constant is known as equilibrium constant. It is denoted by Keq. The equilibrium constant is independent of the initial amount of the reactant and product.

Answer 9

Interpretation Introduction

(b)

Interpretation:

The cellular condition under which glucose-1-phosphate is produced at a high rate is to be stated.

Concept introduction:

When any reaction is at equilibrium then a constant expresses a relationship between the reactant side and the product side. This constant is known as equilibrium constant. It is denoted by Keq. The equilibrium constant is independent of the initial amount of the reactant and product.

Answer 10

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Answer 11

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Answer 12

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Answer 13

Ch. 8 - Prob. 1P Ch. 8 - Prob. 2P Ch. 8 - Prob. 3P Ch. 8 - Prob. 4P Ch. 8 - Prob. 5P Ch. 8 - Prob. 6P Ch. 8 - Prob. 7P Ch. 8 - Prob. 8P Ch. 8 - Prob. 9P Ch. 8 - Prob. 10P

Solution Summary: The author explains the equilibrium constant, which expresses a relationship between the reactant side and the product side. The positive value of free energy predicts that reaction proceeds in the forward direction.

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Chapter 8 Solutions