(a) Interpretation: The activation energy as well as Δ G for the reaction are to be indicated in graph (A). Whether the reaction given in graph (A) is endergonic or exergonic is to be stated. Concept introduction: The rate of reaction is influenced by the nature of reactants, the concentration of reactants, temperature and the presence of catalysts in a reaction. The activation energy ( E a ) is the minimum energy that is required to convert the reactants into products.

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Answer 1

Question

Chapter 8, Problem 10P

Interpretation Introduction

(a)

Interpretation:

The activation energy as well as ΔG for the reaction are to be indicated in graph (A). Whether the reaction given in graph (A) is endergonic or exergonic is to be stated.

Concept introduction:

The rate of reaction is influenced by the nature of reactants, the concentration of reactants, temperature and the presence of catalysts in a reaction. The activation energy (Ea) is the minimum energy that is required to convert the reactants into products.

Interpretation Introduction

(b)

Interpretation:

The activation energy as well as ΔG for the reactions are to be indicated in graph (B). Whether the reaction given in graph (B)is endergonic or exergonic is to be stated.

Concept introduction:

The rate of reaction is influenced by the nature of reactants, the concentration of reactants, temperature and the presence of catalysts in a reaction. The activation energy (Ea) is the minimum energy that is required to convert the reactants into products.

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The beta-lactamase hydrolyzes the lactam-ring in penicillin. Describe the mechanism of hydrolysis, insuring to include the involvement of S, D, & K in the reaction sequence. Please help

To map the active site of beta-lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine. Why doesn't D in this hexapeptide not participate in the hydrolysis of the beta-lactam ring even though S, K, and D are involved in the catalyst?

To map the active site of -lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine. Using the experimental results described above derive the primary sequence of the active site hexapeptide. Please help!

Answer 2

Question

Chapter 8, Problem 10P

Interpretation Introduction

(a)

Interpretation:

The activation energy as well as ΔG for the reaction are to be indicated in graph (A). Whether the reaction given in graph (A) is endergonic or exergonic is to be stated.

Concept introduction:

The rate of reaction is influenced by the nature of reactants, the concentration of reactants, temperature and the presence of catalysts in a reaction. The activation energy (Ea) is the minimum energy that is required to convert the reactants into products.

Interpretation Introduction

(b)

Interpretation:

The activation energy as well as ΔG for the reactions are to be indicated in graph (B). Whether the reaction given in graph (B)is endergonic or exergonic is to be stated.

Concept introduction:

The rate of reaction is influenced by the nature of reactants, the concentration of reactants, temperature and the presence of catalysts in a reaction. The activation energy (Ea) is the minimum energy that is required to convert the reactants into products.

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Answer 3

Question

Chapter 8, Problem 10P

Interpretation Introduction

(a)

Interpretation:

The activation energy as well as ΔG for the reaction are to be indicated in graph (A). Whether the reaction given in graph (A) is endergonic or exergonic is to be stated.

Concept introduction:

The rate of reaction is influenced by the nature of reactants, the concentration of reactants, temperature and the presence of catalysts in a reaction. The activation energy (Ea) is the minimum energy that is required to convert the reactants into products.

Interpretation Introduction

(b)

Interpretation:

The activation energy as well as ΔG for the reactions are to be indicated in graph (B). Whether the reaction given in graph (B)is endergonic or exergonic is to be stated.

Concept introduction:

The rate of reaction is influenced by the nature of reactants, the concentration of reactants, temperature and the presence of catalysts in a reaction. The activation energy (Ea) is the minimum energy that is required to convert the reactants into products.

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Answer 4

Question

Chapter 8, Problem 10P

Interpretation Introduction

(a)

Interpretation:

The activation energy as well as ΔG for the reaction are to be indicated in graph (A). Whether the reaction given in graph (A) is endergonic or exergonic is to be stated.

Concept introduction:

The rate of reaction is influenced by the nature of reactants, the concentration of reactants, temperature and the presence of catalysts in a reaction. The activation energy (Ea) is the minimum energy that is required to convert the reactants into products.

Interpretation Introduction

(b)

Interpretation:

The activation energy as well as ΔG for the reactions are to be indicated in graph (B). Whether the reaction given in graph (B)is endergonic or exergonic is to be stated.

Concept introduction:

The rate of reaction is influenced by the nature of reactants, the concentration of reactants, temperature and the presence of catalysts in a reaction. The activation energy (Ea) is the minimum energy that is required to convert the reactants into products.

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Answer 5

Chapter 8, Problem 10P

Interpretation Introduction

(a)

Interpretation:

The activation energy as well as ΔG for the reaction are to be indicated in graph (A). Whether the reaction given in graph (A) is endergonic or exergonic is to be stated.

Concept introduction:

The rate of reaction is influenced by the nature of reactants, the concentration of reactants, temperature and the presence of catalysts in a reaction. The activation energy (Ea) is the minimum energy that is required to convert the reactants into products.

Interpretation Introduction

(b)

Interpretation:

The activation energy as well as ΔG for the reactions are to be indicated in graph (B). Whether the reaction given in graph (B)is endergonic or exergonic is to be stated.

Concept introduction:

The rate of reaction is influenced by the nature of reactants, the concentration of reactants, temperature and the presence of catalysts in a reaction. The activation energy (Ea) is the minimum energy that is required to convert the reactants into products.

Answer 6

Chapter 8, Problem 10P

Interpretation Introduction

(a)

Interpretation:

The activation energy as well as ΔG for the reaction are to be indicated in graph (A). Whether the reaction given in graph (A) is endergonic or exergonic is to be stated.

Concept introduction:

The rate of reaction is influenced by the nature of reactants, the concentration of reactants, temperature and the presence of catalysts in a reaction. The activation energy (Ea) is the minimum energy that is required to convert the reactants into products.

Answer 7

Chapter 8, Problem 10P

Interpretation Introduction

(a)

Interpretation:

The activation energy as well as ΔG for the reaction are to be indicated in graph (A). Whether the reaction given in graph (A) is endergonic or exergonic is to be stated.

Concept introduction:

The rate of reaction is influenced by the nature of reactants, the concentration of reactants, temperature and the presence of catalysts in a reaction. The activation energy (Ea) is the minimum energy that is required to convert the reactants into products.

Answer 8

Interpretation Introduction

(b)

Interpretation:

The activation energy as well as ΔG for the reactions are to be indicated in graph (B). Whether the reaction given in graph (B)is endergonic or exergonic is to be stated.

Concept introduction:

The rate of reaction is influenced by the nature of reactants, the concentration of reactants, temperature and the presence of catalysts in a reaction. The activation energy (Ea) is the minimum energy that is required to convert the reactants into products.

Answer 9

Interpretation Introduction

(b)

Interpretation:

The activation energy as well as ΔG for the reactions are to be indicated in graph (B). Whether the reaction given in graph (B)is endergonic or exergonic is to be stated.

Concept introduction:

The rate of reaction is influenced by the nature of reactants, the concentration of reactants, temperature and the presence of catalysts in a reaction. The activation energy (Ea) is the minimum energy that is required to convert the reactants into products.

Answer 10

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Answer 11

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Answer 12

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Answer 13

Ch. 8 - Prob. 1P Ch. 8 - Prob. 2P Ch. 8 - Prob. 3P Ch. 8 - Prob. 4P Ch. 8 - Prob. 5P Ch. 8 - Prob. 6P Ch. 8 - Prob. 7P Ch. 8 - Prob. 8P Ch. 8 - Prob. 9P Ch. 8 - Prob. 10P

Solution Summary: The author illustrates how the rate of reaction is influenced by the nature of reactants, concentration, temperature, and the presence of catalysts in a reaction.

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Chapter 8 Solutions