Brock Biology of Microorganisms (15th Edition)
15th Edition
ISBN: 9780134261928
Author: Michael T. Madigan, Kelly S. Bender, Daniel H. Buckley, W. Matthew Sattley, David A. Stahl
Publisher: PEARSON
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Textbook Question
Chapter 7.7, Problem 1MQ
Why are the levels of DnaA protein controlled during the Caulobacter cell cycle?
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Discussion Questions
Answer these discussion questions and submit them as part of your lab report.
Part A: The Effect of Temperature on Enzyme Activity
Graph the volume of oxygen produced against the temperature of the solution.
How is the oxygen production in 30 seconds related to the rate of the reaction?
At what temperature is the rate of reaction the highest? Lowest? Explain.
Why might the enzyme activity decrease at very high temperatures?
Why might a high fever be dangerous to humans?
What is the optimal temperature for enzymes in the human body?
Part B: The Effect of pH on Enzyme Activity
Graph the volume of oxygen produced against the pH of the solution.
At what pH is the rate of reaction the highest? Lowest? Explain.
Why does changing the pH affect the enzyme activity?
Research the enzyme catalase. What is its function in the human body?
What is the optimal pH for the following enzymes found in the human body? Explain. (catalase, lipase (in your stomach),…
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Discussion Questions:
Part One
Why were the plants kept in the dark prior to the experiment? Why is this important?
Why is it important to boil the leaf?
Explain why it was necessary to use boiling alcohol?
What is the purpose of the iodine?
Part Two
What was the purpose of keeping the leaf in the dark and then covering it with a cardboard cut-out?
What conclusions can you draw from this part of the lab?
Part Three
7. In this experiment what was the purpose of adding the soda lime?
8. Why was a sealed bag placed around each plant?
9. What happened in the control plants?
10. What was the result on photosynthesis?
Part Four
11. Why was a variegated leaf used in this experiment?
!2. What conclusions can you draw about starch production in a variegated leaf?
How did the color differences between the two bacterial species you used in this experiment help you determine if the streak plate method you performed was successful?
Chapter 7 Solutions
Brock Biology of Microorganisms (15th Edition)
Ch. 7.1 - What is the utility of a reporter gene?Ch. 7.1 - Prob. 2MQCh. 7.1 - Prob. 1CRCh. 7.2 - Prob. 1MQCh. 7.2 - Prob. 2MQCh. 7.2 - Prob. 1CRCh. 7.3 - What is the divisome?Ch. 7.3 - How does FtsZ find the cell midpoint of a...Ch. 7.3 - What is the role of the penicillin-binding protein...Ch. 7.4 - How does MreB control the shape of a rod-shaped...
Ch. 7.4 - What protein is thought to control the shape of...Ch. 7.4 - What relationships exist between cytoskeletal...Ch. 7.4 - What morphology do cells have that lack MreB or...Ch. 7.5 - Prob. 1MQCh. 7.5 - What is transpeptidation and why is it important...Ch. 7.5 - Prob. 1CRCh. 7.6 - How are different sets of genes expressed in the...Ch. 7.6 - Prob. 2MQCh. 7.6 - Prob. 1CRCh. 7.7 - Why are the levels of DnaA protein controlled...Ch. 7.7 - Prob. 2MQCh. 7.7 - Prob. 1CRCh. 7.8 - Prob. 1MQCh. 7.8 - What is the major transcriptional regulator that...Ch. 7.8 - What is meant by "patterning" during heterocyst...Ch. 7.9 - What are the four basic stages of biofilm...Ch. 7.9 - Besides autoinducer synthesis, what intracellular...Ch. 7.9 - What type of genes does c-di-GMP activate during...Ch. 7.10 - Describe two targets of antibiotics and discuss...Ch. 7.10 - Prob. 2MQCh. 7.10 - Prob. 3MQCh. 7.10 - Prob. 1CRCh. 7.11 - Is persistence a heritable trait?Ch. 7.11 - What prevents the toxin component of TA modules...Ch. 7.11 - Prob. 3MQCh. 7.11 - Prob. 1CRCh. 7 - If DnaA was not regulated in Escherichia coli and...Ch. 7 - Explain how cells exhibiting different phenotypes...Ch. 7 - Describe how you would genetically design a...
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