BIOLOGY
5th Edition
ISBN: 9781265202859
Author: BROOKER
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Chapter 7.5, Problem 1CC
Explain the meaning of the name cytochrome oxidase.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Based on your results, which suspect's DNA best matches the DNA found at the crime scene?
In oxidase test with Pseudomonas aeruginosa, the cell cultures on the slide turn colorless to be
purple after tetra-methyl-p-phenylenediamine dihydrochloride (TMPD) is added. In the
reaction,
OTMPD is electron acceptor
O cytochrome c is the electron source
oxygen is terminal electron acceptor
OH2 produced is electron donor
Chapter 7 Solutions
BIOLOGY
Ch. 7.2 - Core Skill: Connections Look ahead to Table 45.1....Ch. 7.2 - Which organic molecules donate a phosphate group...Ch. 7.2 - Prob. 2CSCh. 7.4 - Prob. 1CCCh. 7.5 - Explain the meaning of the name cytochrome...Ch. 7.6 - Prob. 1CCCh. 7.6 - Prob. 1CSCh. 7.6 - Prob. 1EQCh. 7.6 - CoreSKILL In the experiment of Figure 7.13, what...Ch. 7.6 - Prob. 3EQ
Ch. 7.7 - Prob. 1CCCh. 7 - Which of the following pathways occurs in the...Ch. 7 - Prob. 2TYCh. 7 - Prob. 3TYCh. 7 - Which organic molecule supplies a two-carbon group...Ch. 7 - The ability to diagnose tumors using...Ch. 7 - Prob. 6TYCh. 7 - Certain drugs, which are called ionophores, cause...Ch. 7 - Prob. 8TYCh. 7 - Prob. 9TYCh. 7 - Prob. 10TYCh. 7 - Prob. 1CQCh. 7 - What causes the rotation of the subunit of ATP...Ch. 7 - Core Concept: Energy and Matter How is glucose...Ch. 7 - Discuss the advantages and disadvantages of...Ch. 7 - Prob. 2COQ
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