Genetics: Analysis and Principles
Genetics: Analysis and Principles
5th Edition
ISBN: 9780073525341
Author: Robert J. Brooker Professor Dr.
Publisher: McGraw-Hill Education
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Chapter 7, Problem 20EQ
Summary Introduction

To analyze:

The data from several complementation experiments involving rapid-lysis mutations in genes rIIA and rIIB is given. The strain designated L51 is known to have a mutation in rIIB.

Phage Mixture Complementation
L91 and L65 No
L65 and L62 No
L33 and L47 Yes
L40 and L51 No
L47 and L92 No
L51 and L47 Yes
L51 and L92 Yes
L33 and L40 No
L91 and L92 Yes
L91 and L33 No

Using this information, list which groups of mutations are in the rIIA gene and which groups are in the rIIB gene.

Introduction:

A complementation test is used to test whether mutations are in the different gene or in same genes. Benzer performed a complementation test to study whether rII mutations are in the same gene or in different genes. He used a single trait of phage – the capability to form plaques. In a complementation test, two mutant strains exhibiting a phenotype that is different from the wild type strain are analyzed.

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Chapter 7 Solutions

Genetics: Analysis and Principles

Ch. 7.7 - Prob. 1COMQCh. 7.7 - Prob. 2COMQCh. 7.8 - Prob. 1COMQCh. 7 - 1. The terms conjugation, transduction, and...Ch. 7 - Prob. 2CONQCh. 7 - If you mix together an equal number of F+ and F...Ch. 7 - What is the difference between an F+ and an Hfr...Ch. 7 - 5. What is the role of the origin of transfer...Ch. 7 - 6. What is the role of sex pili during...Ch. 7 - Prob. 7CONQCh. 7 - Prob. 8CONQCh. 7 - Prob. 9CONQCh. 7 - 10. What is cotransduction? What determines the...Ch. 7 - Prob. 11CONQCh. 7 - Prob. 12CONQCh. 7 - Describe the steps that occur during bacterial...Ch. 7 - Prob. 14CONQCh. 7 - Prob. 15CONQCh. 7 - Antibiotics such as tetracycline, streptomycin,...Ch. 7 - What does the term complementation mean? If two...Ch. 7 - Prob. 18CONQCh. 7 - Prob. 19CONQCh. 7 - Prob. 1EQCh. 7 - 2. In the experiment of Figure 7.1, Lederberg and...Ch. 7 - Explain how a U-tube apparatus can distinguish...Ch. 7 - Prob. 4EQCh. 7 - 5. In a conjugation experiment, what is meant by...Ch. 7 - In your laboratory, you have an F strain of E....Ch. 7 - 7. As mentioned in question 2 of More Genetic...Ch. 7 - An Hfr strain that is hisE+ and pheA+ was mixed...Ch. 7 - Acridine orange is a chemical that inhibits the...Ch. 7 - Prob. 10EQCh. 7 - Prob. 11EQCh. 7 - Lets suppose a new strain of P1 phage has been...Ch. 7 - If two bacterial genes are 0.6 minute apart on the...Ch. 7 - 14. In a cotransduction experiment involving P1,...Ch. 7 - Prob. 15EQCh. 7 - Prob. 16EQCh. 7 - E17. If you took a pipette tip and removed a phage...Ch. 7 - Prob. 18EQCh. 7 - E19. In the experimental strategy described in...Ch. 7 - Prob. 20EQCh. 7 - Prob. 21EQCh. 7 - 1. Discuss the advantages of the genetic analysis...Ch. 7 - Complementation occurs when two defective alleles...
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