Microbiology: Principles and Explorations
Microbiology: Principles and Explorations
10th Edition
ISBN: 9781119390114
Author: Black
Publisher: WILEY
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Chapter 6, Problem 2.2SC
Summary Introduction

To describe: The reason for which the inhibitors that bind to the allosteric site called are non-competitive inhibitors.

Introduction: Microbial metabolism is a way through which the microorganisms acquire the energy as well as nutrients (for example, the carbon) they require for living and reproducing. A variety of metabolic strategies are used by microbes, and microbial species could often be distinguished from one another depending on the metabolic characteristics.   

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You perform Michaelis-Menten kenetics on i) trypsin and ii) a mutant form of the same enzyme (a single amino acid has been changed). The specificity constant for the mutant was 10 times larger than for trypsin. a) Define the specificity constant and explain using this definition how a larger value might occur. b) Explain how, if at all, a non-competitive inhibitor of trypsin would affect the specificity constant.
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