Microbiology: Principles and Explorations
10th Edition
ISBN: 9781119390114
Author: Black
Publisher: WILEY
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Chapter 6, Problem 2.2SC
Summary Introduction
To describe: The reason for which the inhibitors that bind to the allosteric site called are non-competitive inhibitors.
Introduction: Microbial
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You perform Michaelis-Menten kenetics on i) trypsin and ii) a mutant form of the same enzyme (a single amino acid has been changed). The specificity constant for the mutant was 10 times larger than for trypsin.
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b) Explain how, if at all, a non-competitive inhibitor of trypsin would affect the specificity constant.
Chapter 6 Solutions
Microbiology: Principles and Explorations
Ch. 6 - How are photosynthesis and respiration related to...Ch. 6 - What is the main difference between...Ch. 6 - Distinguish between coenzyme and cofactor. How are...Ch. 6 - Prob. 2.2SCCh. 6 - Prob. 3.1SCCh. 6 - If four actual molecules of ATP are produced for...Ch. 6 - Prob. 3.3SCCh. 6 - Prob. 3.4SCCh. 6 - Prob. 4.1SCCh. 6 - What is returned to chlorophyll in cyclic...
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- What is meant by the binding problem, and what is necessary for binding to occur?arrow_forwardHow can we identify a noncompetitive inhibitor?arrow_forwardWhy is it advantageous for two control mechanisms —allosteric control and covalent modification— to be involved in the metabolism of glycogen?arrow_forward
- Why are protein synthesis inhibitors typically broad spectrum? What might prevent a protein synthesis inhibitor from being broad-spectrum?arrow_forwardIn which reversible inhibitors, adding more substrates can revert the inhibition? Why?arrow_forwardWhy Phenylmethylsulfonyl fluoride (PMSF, shown in Fig.) does not inhibit aspartate protease as potent as serine protease?arrow_forward
- What is feedback inhibition? Why is it a useful property?arrow_forwardWithin an enzyme active site, how does the aspartate carboxyl group activate the histidine nitrogen and convert it into a stronger base?arrow_forwardCertain glutamine analogs irreversibly inactivate enzymes that bind glutamine. Identify the nucleotide biosynthetic intermediates that accumulate in the presence of those compounds.arrow_forward
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