Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
12th Edition
ISBN: 9780134605203
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
bartleby

Concept explainers

bartleby

Videos

Textbook Question
Book Icon
Chapter 54, Problem 1Q

Why are plates with 25 to 250 colonies used for calculations?

Expert Solution & Answer
Check Mark
Summary Introduction

To write:

The reason for the use of plates with 25 to 250 colonies for the calculations.

Introduction:

A culture plate:

In biology, it is a low and flat-bottomed laboratory container. It is useful to grow a layer of organisms on a thin nutrient medium layer. These include the molds, bacterias, and other cells. The most ordinary types of culture plates are the multiwell plate and the Petri dish.

Explanation of Solution

A colony can be defined as a visible mass of microbes that all originate from a single mother cell. Thus, a colony is formed of a clone of bacteria which are all genetically similar.

Plates with 25 to 250 colonies:

The FDA Bacterial Analytical Manual (BAM) recommends 25-250 CFU/plate as a countable range. If there are less than 25 colonies then it is too little count that it is not a statistically significant number of colonies.

While if there are more than 250 colonies then it is too many to count as it is impossible to recognize whether the colonies are parted.

Plates having 25-250 colonies should be used for calculations as they provide most precise results.

Conclusion

Plates having 25-250 colonies should be used for calculations as they provide most precise results.

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!
Students have asked these similar questions
We will be starting a group project next week where you and your group will research and ultimately present on a current research article related to the biology of a pathogen that infects humans. The article could be about the pathogen itself, the disease process related to the pathogen, the immune response to the pathogen, vaccines or treatments that affect the pathogen, or other biology-related study about the pathogen. I recommend that you choose a pathogen that is currently interesting to researchers, so that you will be able to find plenty of articles about it. Avoid choosing a historical disease that no longer circulates. List 3 possible pathogens or diseases that you might want to do for your group project.
not use ai please
DNK dagi nukleotidlar va undan sintezlangan oqsildagi peptid boglar farqi 901 taga teng bo'lib undagi A jami H boglardan 6,5 marta kam bo'lsa DNK dagi jami H bog‘lar sonini toping
Knowledge Booster
Background pattern image
Biology
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.
Similar questions
SEE MORE QUESTIONS
Recommended textbooks for you
Text book image
Basic Clinical Laboratory Techniques 6E
Biology
ISBN:9781133893943
Author:ESTRIDGE
Publisher:Cengage
Text book image
Aquaculture Science
Biology
ISBN:9781133558347
Author:Parker
Publisher:Cengage
Text book image
Science Of Agriculture Biological Approach
Biology
ISBN:9780357229323
Author:Herren
Publisher:Cengage
Text book image
Biology Today and Tomorrow without Physiology (Mi...
Biology
ISBN:9781305117396
Author:Cecie Starr, Christine Evers, Lisa Starr
Publisher:Cengage Learning
Text book image
Principles Of Radiographic Imaging: An Art And A ...
Health & Nutrition
ISBN:9781337711067
Author:Richard R. Carlton, Arlene M. Adler, Vesna Balac
Publisher:Cengage Learning
Text book image
Case Studies In Health Information Management
Biology
ISBN:9781337676908
Author:SCHNERING
Publisher:Cengage
Bacterial Structure and Functions; Author: Osmosis;https://www.youtube.com/watch?v=b15Hy3jCPDs;License: Standard youtube license