Concept explainers
Why are plates with 25 to 250 colonies used for calculations?
To write:
The reason for the use of plates with 25 to 250 colonies for the calculations.
Introduction:
A culture plate:
In biology, it is a low and flat-bottomed laboratory container. It is useful to grow a layer of organisms on a thin nutrient medium layer. These include the molds, bacterias, and other cells. The most ordinary types of culture plates are the multiwell plate and the Petri dish.
Explanation of Solution
A colony can be defined as a visible mass of microbes that all originate from a single mother cell. Thus, a colony is formed of a clone of bacteria which are all genetically similar.
Plates with 25 to 250 colonies:
The FDA Bacterial Analytical Manual (BAM) recommends 25-250 CFU/plate as a countable range. If there are less than 25 colonies then it is too little count that it is not a statistically significant number of colonies.
While if there are more than 250 colonies then it is too many to count as it is impossible to recognize whether the colonies are parted.
Plates having 25-250 colonies should be used for calculations as they provide most precise results.
Plates having 25-250 colonies should be used for calculations as they provide most precise results.
Want to see more full solutions like this?
Chapter 54 Solutions
Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
- One of the ways for a cell to generate ATP is through the oxidative phosphorylation. In oxidative phosphorylation 3 ATP are produced from every one NADH molecule. In respiration, every glucose molecule produces 10 NADH molecules. If a cell is growing on 5 glucose molecules, how much ATP can be produced using oxidative phosphorylation/aerobic respiration?arrow_forwardIf a cell is growing on 5 glucose molecules, how much ATP can be produced using oxidative phosphorylation/aerobic respiration?arrow_forwardHow do i know which way the arrows go?arrow_forward
- Identify the indicated structure (Saprolegnia). a. antheridium O b. oospore c.sperm d. auxospore e. tetraspore Of. zygosporearrow_forwardUsing information from the primary literature (several references have been provided as a starting point below) please answer the following question: Based on your review of the literature on rewilding, what are the major scientific pros and cons for rewilding? Please note that the focus of this assignment are the (biological) scientific issues associated with rewilding. As will be discussed in class, there are a number of non-scientific issues involved or implicated in rewilding, all ultimately affecting the public acceptability of rewilding. Although these issues are important – indeed, critical – in this assignment you should focus on the biological science issues and questions. Details: You must enumerate at least two pros and at least two cons. Your answer should be no more than 500 well-chosen words, excluding references. Think carefully about how best to organize and structure your answer. Aim for high information density: say a lot, but say it succinctly. Recall Nietzche’s…arrow_forwardUsing information from the primary literature (several references have been provided as a starting point below) please answer the following question: Based on your review of the literature on rewilding, what are the major scientific pros and cons for rewilding? Please note that the focus of this assignment are the (biological) scientific issues associated with rewilding. As will be discussed in class, there are a number of non-scientific issues involved or implicated in rewilding, all ultimately affecting the public acceptability of rewilding. Although these issues are important – indeed, critical – in this assignment you should focus on the biological science issues and questions. Details: You must enumerate at least two pros and at least two cons. Your answer should be no more than 500 well-chosen words, excluding references. Think carefully about how best to organize and structure your answer. Aim for high information density: say a lot, but say it succinctly. Recall Nietzche’s…arrow_forward
- Now draw a rough sketch of what the control data might look like if in addition to the specific binding, there was also a considerable amount of nonspecific binding (again using a normal dose/response curve) (do % total bound ligand vs concentration)arrow_forwardWhat are functions of cuboidal cells in the kidney? Select all that apply. Concentration of gases Dilution of chemicals Secretion of molecules Nutrition to tissues Support of tissues Absorption of moleculesarrow_forwardquestion1 In plants, epithelial tissue is only found as the outermost cell layer and acts as a barrier. In humans, epithelial tissue is found inside the body as well as on the surface. What function(s) does/do epithelial tissue carry out in humans? Select all that apply. Waste storage Filtration Oxygen transport Protection Diffusion Osmosis Absorptionarrow_forward
- Biology Today and Tomorrow without Physiology (Mi...BiologyISBN:9781305117396Author:Cecie Starr, Christine Evers, Lisa StarrPublisher:Cengage LearningPrinciples Of Radiographic Imaging: An Art And A ...Health & NutritionISBN:9781337711067Author:Richard R. Carlton, Arlene M. Adler, Vesna BalacPublisher:Cengage LearningCase Studies In Health Information ManagementBiologyISBN:9781337676908Author:SCHNERINGPublisher:Cengage