Campbell Biology: Australian And New Zealand Edition + Mastering Biology With Etext
11th Edition
ISBN: 9781488687075
Author: Lisa, A. Urry
Publisher: PEARSON
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Textbook Question
Chapter 53, Problem 14TYU
WRITE ABOUT A THEME: INTERACTIONS In a short essay (100-150 words), identify the factor or factors in Figure 53.18 that you think may ultimately be most important for density- dependent population regulation in humans, and explain your reasoning.
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1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?
3) Why are the cells incubated at 42°C?
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Chapter 53 Solutions
Campbell Biology: Australian And New Zealand Edition + Mastering Biology With Etext
Ch. 53.1 - DRAW IT Each female of a particular fish species...Ch. 53.1 - Prob. 2CCCh. 53.1 - Prob. 3CCCh. 53.2 - Explain why a constant per capita rate of growth...Ch. 53.2 - Prob. 2CCCh. 53.2 - Prob. 3CCCh. 53.3 - Explain why a population that fits the logistic...Ch. 53.3 - WHAT IF? Given the latitudinal differences in...Ch. 53.3 - Prob. 3CCCh. 53.4 - Identify three key life history traits, and give...
Ch. 53.4 - Prob. 2CCCh. 53.4 - Prob. 3CCCh. 53.5 - Prob. 1CCCh. 53.5 - WHAT IF? Suppose you were studying a species that...Ch. 53.5 - Prob. 3CCCh. 53.6 - How does a human population's age structure affect...Ch. 53.6 - How have the rate and number of people added to...Ch. 53.6 - WHAT IF? Type "personal ecological footprint...Ch. 53 - Gray whales (Eschrichtius robustus) gather each...Ch. 53 - Suppose one population has an r that is twice as...Ch. 53 - Prob. 53.3CRCh. 53 - Prob. 53.4CRCh. 53 - Density-dependent factors regulate population...Ch. 53 - The human population is no longer growing...Ch. 53 - Population ecologists follow the fate of same-age...Ch. 53 - A population's carrying capacity (A) may change as...Ch. 53 - Scientific study of the population cycles of the...Ch. 53 - Analyzing ecological footprints reveals that (A)...Ch. 53 - Based on current growth rates, Earth's human...Ch. 53 - The observation that members of a population are...Ch. 53 - According to the logistic growth equation...Ch. 53 - During exponential growth, a population always (A)...Ch. 53 - Which of the following statements about human...Ch. 53 - Prob. 10TYUCh. 53 - EVOLUTION CONNECTION Contrast the selective...Ch. 53 - Prob. 12TYUCh. 53 - Prob. 13TYUCh. 53 - WRITE ABOUT A THEME: INTERACTIONS In a short essay...Ch. 53 - SYNTHESIZE YOUR KNOWLEDGE Locusts (grasshoppers in...
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