To predict: How genetic drift and natural selection could affect a population, where a sudden change in the condition of the environment, has caused a drop in the carrying capacity of the population. Concept introduction : K or the Carrying capacity is defined as the environment that can sustain maximum size of the population . The distribution of organisms on land is influenced greatly by the climatic conditions. Climatic condition is defined as the long term weather condition that prevails in an area for a longer period. Carrying capacity of a population can be affected because of the natural selection and genetic drift.

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Chapter 53.3, Problem 3CC

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To predict: How genetic drift and natural selection could affect a population, where a sudden change in the condition of the environment, has caused a drop in the carrying capacity of the population.

Concept introduction: K or the Carrying capacity is defined as the environment that can sustain maximum size of the population. The distribution of organisms on land is influenced greatly by the climatic conditions. Climatic condition is defined as the long term weather condition that prevails in an area for a longer period. Carrying capacity of a population can be affected because of the natural selection and genetic drift.

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Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1) What differences would you expect to see between the…

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