Campbell Biology Plus Mastering Biology with Pearson eText - Access Card Package (11th Edition)
11th Edition
ISBN: 9780134082318
Author: Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, Jane B. Reece
Publisher: PEARSON
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Chapter 52.4, Problem 3CC
MAKE CONNECTIONS → Hawaiian silverswords under-went a remarkable adaptive radiation after their ancestor reached Hawaii, while the islands were still young (see Figure 25.22). Would you expect the cattle egret to undergo a similar adaptive radiation in the Americas (see Figure 52.18)? Explain.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Based on your results, which suspect's DNA best matches the DNA found at the crime scene?
Chapter 52 Solutions
Campbell Biology Plus Mastering Biology with Pearson eText - Access Card Package (11th Edition)
Ch. 52.1 - Explain how the sun's unequal heating of Earth's...Ch. 52.1 - What are some of the differences in microclimate...Ch. 52.1 - WHAT IF? Changes in Earth's climate at the end of...Ch. 52.1 - MAKE CONNECTIONS Focusing just on the effects of...Ch. 52.2 - Based on the climograph in Figure 52.10, what...Ch. 52.2 - Using Figure 52.12, identify the natural biome in...Ch. 52.2 - WHAT IF? If global warming increases average...Ch. 52.3 - Why are phytoplankton, and not benthic algae or...Ch. 52.3 - Prob. 2CCCh. 52.3 - MAKE CONNECTIONS As noted in Figure 52.15, the...
Ch. 52.4 - Give examples of human actions that could expand a...Ch. 52.4 - WHAT IF? You suspect that deer are restricting...Ch. 52.4 - MAKE CONNECTIONS Hawaiian silverswords under-went...Ch. 52.5 - Describe a scenario showing how ecological change...Ch. 52.5 - MAKE CONNECTIONS Commercial fisheries target...Ch. 52 - Suppose global air circulation suddenly reversed,...Ch. 52 - Prob. 52.2CRCh. 52 - Prob. 52.3CRCh. 52 - Interactions between organisms and the environment...Ch. 52 - Suppose humans introduced a species to a new...Ch. 52 - Level 1: Knowledge/Comprehension 1. Which of the...Ch. 52 - Which lake zone would be absent in a very shallow...Ch. 52 - Which of the following is characteristic of most...Ch. 52 - The oceans affect the biosphere in all of the...Ch. 52 - Which statement about dispersal is false? (A)...Ch. 52 - When climbing a mountain, we can observe...Ch. 52 - Prob. 7TYUCh. 52 - Prob. 8TYUCh. 52 - Prob. 9TYUCh. 52 - EVOLUTION CONNECTION Discuss how the distribution...Ch. 52 - SCIENTIFIC INQUIRY Jens Clausen and colleagues, at...Ch. 52 - WRITE ABOUT A THEME: INTERACTIONS Global warming...Ch. 52 - SYNTHESIZE YOUR KNOWLEDGE If you were to hike up...
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