Chapter 5, Problem 5P

Interpretation Introduction

(a) Interpretation:

To determine the titration diagram and to find the charge and isoelectric point of a-melanotropin.

Introduction:

The titration curve of the amino acids can be used to determine the isoelectric point of the amino acids. The isoelectric point is the point, at which the net charge on the amino acids becomes zero.

Interpretation Introduction

(b) Interpretation:

Calculate the charge on the a-melanotropin at pH 11, 5 and 1.

Introduction:

The net charge on the amino acid or the protein can be extrapolated by the titration graph. The net charge on the protein or any amino acid sequence depends on the charge on all the amino acid residue present in the chain.

Interpretation Introduction

(c) Interpretation:

The calculate the isoelectric potential.

Introduction:

The isoelectric point (pl) is the pH at which the molecule has net zero charge.