Fundamentals of Biochemistry: Life at the Molecular Level
5th Edition
ISBN: 9781118918401
Author: Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher: WILEY
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Chapter 5, Problem 33CQ
Summary Introduction
To determine: The position of disulfide bonds in the intact polypeptide.
Concept introduction: Proteins are made up of one or more amino acids linked together to form a chain. The building blocks of the protein is called amino acid. Amino acids are termed as residues if they release a hydrogen ion from the
Given:
- Treatment of a polypeptide with 2-mercaptoethanol yields two polypeptides: Ala-Val-Cys-Arg-Thr-Gly-Cys-Lys-Asn-Phe-Leu, Tyr-Lys-Cys-Phe-Arg-His-Thr-Lys-Cys-Ser.
- Treatment of the intact polypeptide with trypsin yields seven peptide fragments: Ala, Arg, Cys2, Ser, Val; Arg, Cys2, Gly, Lys, Thr, Phe; Asn, Leu, Phe; His, Lys, Thr; Lys, Tyr.
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Please help with this Mass Spectrometry Question. Thank you
For the mass spec. shown in the attached image, please determine and give the amino acid sequence of the pentapeptide. Show which end is the amino terminus and which is the carboxy terminus. How does one arrive at the solution?
Draw a tripeptide of your choosing at pH 7. Have the N-terminus on the left and the C-terminus on the right. Then:
Draw a triangle around the α-carbons.
Draw a box around the R-groups.
Circle the atoms capable of hydrogen bonding.
Highlight the atoms involved in the formation of the peptide bonds.
What type of structure have you drawn? (primary, secondary, tertiary or quaternary protein structure).
2. For the flow of fluid over a flat membrane of length 10cm,
determine the length-average mass transfer coefficient. The
relevant properties of the system are u=0.01cm²/s, D=5 x 106
cm²/s, and v = 5.0 cm/s.
Chapter 5 Solutions
Fundamentals of Biochemistry: Life at the Molecular Level
Ch. 5 - Prob. 1ECh. 5 - Prob. 2ECh. 5 - 3. Which peptide has greater absorbance at 280...Ch. 5 - 4. Protein X has an absorptivity of 0.4 mL · mg?1...Ch. 5 - 5. You are using ammonium sulfate to purify...Ch. 5 - Prob. 6ECh. 5 - 7. (a) In what order would the amino acids Arg,...Ch. 5 - Prob. 8ECh. 5 - 9. Explain why a certain protein has an apparent...Ch. 5 - 10. Determine the subunit composition of a protein...
Ch. 5 - 11. Explain why a protein, which has a...Ch. 5 - 12. A protein has an apparent mass of 800 kD by...Ch. 5 - 13. Explain why the dansyl chloride treatment of a...Ch. 5 - 14. Identify the tint residue obtained by Edman...Ch. 5 - 15. A pentapeptide has the sequence NNKNN (using...Ch. 5 - 16. The peptide in Problem 15 was sequenced by...Ch. 5 - 17. In site-directed mutagenesis experiments, Gly...Ch. 5 - 18. Below is a list of the first 10 residues of...Ch. 5 - Prob. 19ECh. 5 - Prob. 20ECh. 5 - Prob. 21CQCh. 5 - Prob. 22CQCh. 5 - 23. What fractionation procedure could be used to...Ch. 5 - Prob. 24CQCh. 5 - 25. Purification tables are often used to keep...Ch. 5 - Prob. 26CQCh. 5 - 27. You must cleave the following peptide into...Ch. 5 - 28. You wish to determine the sequence of a...Ch. 5 - Prob. 29CQCh. 5 - Prob. 30CQCh. 5 - 31. You wish to determine the sequence of a short...Ch. 5 - Prob. 32CQCh. 5 - Prob. 33CQCh. 5 - 34. You wish to sequence the light chain of a...Ch. 5 - Prob. 1MTE
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