Chapter 5, Problem 22CQ

(a)

Summary Introduction

To explain: The reason why cells die when incubated with supernatant.

Concept introduction: Ultracentrifugation is a technique that involves the application of centrifugal force to separate molecules from the solution based on their viscosity of the medium, shape, density, size, and the rotor speed. Ammonium sulfate precipitation is the most common and effective method for fractionating proteins.

(b)

Summary Introduction

To explain: The ways to overcome from dying of cells when incubated with supernatant.

Concept introduction: Ultracentrifugation is a technique that involves the application of centrifugal force to separate molecules from the solution based on their viscosity of the medium, shape, density, size, and the rotor speed. Ammonium sulfate precipitation is the most common and effective method for fractionating proteins.