Microbiology: A Laboratory Manual (11th Edition)
11th Edition
ISBN: 9780134098630
Author: James G. Cappuccino, Chad T. Welsh
Publisher: PEARSON
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Chapter 5, Problem 26E.2RQ
Summary Introduction
To describe: The litmus milk reaction that might occur when proteins are
Introduction: Litmus milk is a medium that is useful to differentiate between various species of bacteria. The lactose present in the medium serves as sugar for microbial growth, the litmus serves as a pH indicator. Casein is a protein present in the medium that is metabolized by various bacterial species.
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19. On the diagram below
a. Label the three pictures as: DNA; polypeptide; or RNA.
b. Label the arrows as: translation or transcription/RNA processing.
c. Add the following details to the diagram.
Promoter region
TATA box
Transcription start site
Transcription terminator
Intron (A,B,C,D)
Exons (1,2,3,4,5)
Splice sites
5' cap
5' UTR (untranslated region)
3' poly A tail
3' UTR (untranslated region)
Translational start (AUG)
Translational stop (UGA, UAG, or UAA)
N and C ends of polypeptide
0000
Match the letter labels in the figure below to the terms. Some letter
labels are not used.
MNNNNNNIN
M
C
B
A
M
D
F
E
H
K
G
8
The diagram below illustrates a quorum sensing pathway from Staphylococcus aureus. Please answer the following questions.
1. Autoinduction is part of the quorum sensing system. Which promoter (P2 or P3) is critical for autoinduction?
2)This staphylococcus aureus grows on human wounds, causing severe infections. You would like to start a clinical trial to treat these wound infections. Please describe:
a) What molecule do you recommend for the trial. Why?
b) Your trial requires that Staphylococcus aureus be isolated from the wound and submitted to genome sequencing before admittance. Why? What are you testing for?
3) If a mutation arises where the Promoter P3 is constitutively active, how would that influence sensitivity to AIP? Please explain your rationale.
4) This pathway is sensitive to bacterial cell density. Describe two separate mutation that would render the pathway active independent of cell density. Briefly explain your rationale.
Mutation 1
Mutation 2
Chapter 5 Solutions
Microbiology: A Laboratory Manual (11th Edition)
Ch. 5 - Why is the catalytic activity of enzymes essential...Ch. 5 - Why are microorganisms able to cause dairy...Ch. 5 - Give a reason why it is necessary for...Ch. 5 - Prob. 21E.1RQCh. 5 - Prob. 21E.2RQCh. 5 - Prob. 21E.3RQCh. 5 - Prob. 21E.4RQCh. 5 - Prob. 21E.5RQCh. 5 - What is the purpose of the TSI test?
Ch. 5 - Explain why the TSI medium contains a lower...
Ch. 5 - Prob. 22E.3RQCh. 5 - Prob. 22E.4RQCh. 5 - Explain why the test observations must be made...Ch. 5 - Discuss the medical significance of the IMViC...Ch. 5 - Explain the chemical mechanism for detecting...Ch. 5 -
Explain the chemical mechanism for detecting...Ch. 5 - Prob. 23E.4RQCh. 5 - The end products of tryptophan degradation are...Ch. 5 - Prob. 23E.6RQCh. 5 - Distinguish between the types of substrates...Ch. 5 - Prob. 24E.2RQCh. 5 - Prob. 24E.3RQCh. 5 - Prob. 24E.4RQCh. 5 - Prob. 24E.5RQCh. 5 - Explain the mechanism of urease activity.
Ch. 5 - Prob. 25E.2RQCh. 5 - Prob. 25E.3RQCh. 5 - Prob. 25E.4RQCh. 5 - Distinguish between acid and rennet curds.
Ch. 5 - Prob. 26E.2RQCh. 5 - Prob. 26E.3RQCh. 5 - Can a litmus milk culture show a pink band at the...Ch. 5 - Prob. 26E.5RQCh. 5 - Prob. 27E.1RQCh. 5 - Prob. 27E.2RQCh. 5 - Prob. 27E.3RQCh. 5 - Prob. 27E.4RQCh. 5 - Discuss the relationship between an organism’s...Ch. 5 - Explain the toxic effect of O2 on strict...Ch. 5 - Prob. 28E.2RQCh. 5 - Would catalase be classified as an endoenzyme or...Ch. 5 - Prob. 28E.4RQCh. 5 - Prob. 29E.1RQCh. 5 - Why are strict aerobes oxidase-positive?
Ch. 5 - Prob. 29E.3RQCh. 5 - Prob. 29E.4RQCh. 5 - Prob. 29E.5RQCh. 5 - Prob. 30E.1RQCh. 5 - Prob. 30E.2RQCh. 5 - What is the function of ferric chloride in the...Ch. 5 - Prob. 30E.4RQCh. 5 - Prob. 30E.5RQ
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