Microbiology: A Laboratory Manual (11th Edition)
11th Edition
ISBN: 9780134098630
Author: James G. Cappuccino, Chad T. Welsh
Publisher: PEARSON
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Chapter 5, Problem 27E.3RQ
Summary Introduction
To explain: How to interpret the result when a culture fails to undergo a color change even after the addition of solutions A and B in nitrate reduction test.
Introduction: Nitrate reduction test is performed to determine the capability of an organism to reduce nitrate to nitrite by using an enzyme called nitrate reductase. If the medium turns red after adding the nitrate reagents then it is said to be positive for nitrate reduction.
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A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation?
The cell membranes are more resistant than normal.
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A solution of 0.1 M NaCl had been added instead of water.
Not enough water had been added to the red blood cell pellet.
The man had sickle-cell anaemia.
A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation?
The cell membranes are more resistant than normal.
An isotonic solution had been added instead of water.
A solution of 0.1 M NaCl had been added instead of water.
Not enough water had been added to the red blood cell pellet.
The man had sickle-cell anaemia.
With reference to their absorption spectra of the oxy haemoglobin intact line) and deoxyhemoglobin (broken line) shown in Figure 2 below, how would you best explain the reason why there are differences in the major peaks of the spectra? Figure 2. SPECTRA OF OXYGENATED AND DEOXYGENATED HAEMOGLOBIN OBTAINED WITH THE RECORDING SPECTROPHOTOMETER 1.4 Abs < 0.8 06 0.4 400 420 440 460 480 500 520 540 560 580 600 nm 1. The difference in the spectra is due to a pH change in the deoxy-haemoglobin due to uptake of CO2- 2. There is more oxygen-carrying plasma in the oxy-haemoglobin sample. 3. The change in Mr due to oxygen binding causes the oxy haemoglobin to have a higher absorbance peak. 4. Oxy-haemoglobin is contaminated by carbaminohemoglobin, and therefore has a higher absorbance peak 5. Oxy-haemoglobin absorbs more light of blue wavelengths and less of red wavelengths than deoxy-haemoglobin
Chapter 5 Solutions
Microbiology: A Laboratory Manual (11th Edition)
Ch. 5 - Why is the catalytic activity of enzymes essential...Ch. 5 - Why are microorganisms able to cause dairy...Ch. 5 - Give a reason why it is necessary for...Ch. 5 - Prob. 21E.1RQCh. 5 - Prob. 21E.2RQCh. 5 - Prob. 21E.3RQCh. 5 - Prob. 21E.4RQCh. 5 - Prob. 21E.5RQCh. 5 - What is the purpose of the TSI test?
Ch. 5 - Explain why the TSI medium contains a lower...
Ch. 5 - Prob. 22E.3RQCh. 5 - Prob. 22E.4RQCh. 5 - Explain why the test observations must be made...Ch. 5 - Discuss the medical significance of the IMViC...Ch. 5 - Explain the chemical mechanism for detecting...Ch. 5 -
Explain the chemical mechanism for detecting...Ch. 5 - Prob. 23E.4RQCh. 5 - The end products of tryptophan degradation are...Ch. 5 - Prob. 23E.6RQCh. 5 - Distinguish between the types of substrates...Ch. 5 - Prob. 24E.2RQCh. 5 - Prob. 24E.3RQCh. 5 - Prob. 24E.4RQCh. 5 - Prob. 24E.5RQCh. 5 - Explain the mechanism of urease activity.
Ch. 5 - Prob. 25E.2RQCh. 5 - Prob. 25E.3RQCh. 5 - Prob. 25E.4RQCh. 5 - Distinguish between acid and rennet curds.
Ch. 5 - Prob. 26E.2RQCh. 5 - Prob. 26E.3RQCh. 5 - Can a litmus milk culture show a pink band at the...Ch. 5 - Prob. 26E.5RQCh. 5 - Prob. 27E.1RQCh. 5 - Prob. 27E.2RQCh. 5 - Prob. 27E.3RQCh. 5 - Prob. 27E.4RQCh. 5 - Discuss the relationship between an organism’s...Ch. 5 - Explain the toxic effect of O2 on strict...Ch. 5 - Prob. 28E.2RQCh. 5 - Would catalase be classified as an endoenzyme or...Ch. 5 - Prob. 28E.4RQCh. 5 - Prob. 29E.1RQCh. 5 - Why are strict aerobes oxidase-positive?
Ch. 5 - Prob. 29E.3RQCh. 5 - Prob. 29E.4RQCh. 5 - Prob. 29E.5RQCh. 5 - Prob. 30E.1RQCh. 5 - Prob. 30E.2RQCh. 5 - What is the function of ferric chloride in the...Ch. 5 - Prob. 30E.4RQCh. 5 - Prob. 30E.5RQ
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