Biochemistry
Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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Chapter 4, Problem 9P
Interpretation Introduction

Interpretation:

The minimum length of a DNA sequence that will appear only once in the human genome needs to be determined, if the human genome contains 3 billion nucleotides arranged in a vast array of the sequence. This is to be taken into account that only one strand and all four nucleotides have the same probability of appearance.

Concept introduction:

Deoxyribonucleic acid (DNA) is a molecule consisting of two strands that twist around each other to create a double strand DNA containing directions for the development, working, growth and breeding of all recognized species and many bacteria. It is also recognized as polynucleotides. The two DNA sections consist of simpler monomeric blocks called nucleotides.

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a) What are the differences between the Direct & Indirect Immunofluorescence Assays? (0.5 mark) b) What are the advantages of the Indirect Immunofluorescence Assays? (0.5 mark) c) A Super-Resolution Imaging Technique was developed in 2018 using imidazole, a His-tag ligand conjugated with a fluorophore to report the presence of a recombinant His-tag protein target, (Sci Rep, 2018, 8:5507). How does this technique improve the image quality? (2 marks)
a) What are the differences between the Direct & Indirect Immunofluorescence Assays?  b) What are the advantages of the Indirect Immunofluorescence Assays?  c) A Super-Resolution Imaging Technique was developed in 2018 using imidazole, a His-tag ligand conjugated with a fluorophore to report the presence of a recombinant His-tag protein target, (Sci Rep, 2018, 8:5507). How does this technique improve the image quality?
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