Biochemistry
Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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Chapter 4, Problem 27P
Interpretation Introduction

(a)

Interpretation:

The DNA Polymerase and RNA Polymerase from E. coli needs to be compared on the basis of the activated precursors.

Concept introduction:

DNA Polymerase is an enzyme that helps in the formation of DNA from deoxyribonucleotide, a unit structure of a DNA. This enzyme helps in replication of the DNA strand to form the double helical structure of the DNA. DNA Polymerase reads the existing strand of DNA and synthesizes the new strand which is a replica of the existing strand.

RNA Polymerase is an enzyme that initiates RNA synthesis from a DNA template. This RNA polymerase enzyme opens the double-stranded DNA to expose one strand of the nucleotide so that it can be used as the template for RNA Synthesis.

Interpretation Introduction

(b)

Interpretation:

The DNA Polymerase and RNA Polymerase from E. coli needs to be compared on the basis of

the direction of chain elongation.

Concept introduction:

DNA Polymerase is an enzyme that helps in the formation of DNA from deoxyribonucleotide, a unit structure of a DNA. This enzyme helps in replication of the DNA strand to form the double helical structure of the DNA. DNA Polymerase reads the existing strand of DNA and synthesizes the new strand which is a replica of the existing strand.

RNA Polymerase is an enzyme that initiates RNA synthesis from a DNA template. This RNA polymerase enzyme opens the double-stranded DNA to expose one strand of the nucleotide so that it can be used as the template for RNA Synthesis.

Interpretation Introduction

(c)

Interpretation:

The DNA Polymerase and RNA Polymerase from E.coli needs to be compared on the basis of

conservation of template.

Concept introduction:

DNA Polymerase is an enzyme that helps in the formation of DNA from deoxyribonucleotide, a unit structure of a DNA. This enzyme helps in replication of the DNA strand to form the double helical structure of the DNA. DNA Polymerase reads the existing strand of DNA and synthesizes the new strand which is a replica of the existing strand.

RNA Polymerase is an enzyme that initiates RNA synthesis from a DNA template. This RNA polymerase enzyme opens the double-stranded DNA to expose one strand of the nucleotide so that it can be used as the template for RNA Synthesis.

Interpretation Introduction

(d)

Interpretation:

The DNA Polymerase and RNA Polymerase from E.coli needs to be compared on the basis of

need for primer.

Concept introduction:

DNA Polymerase is an enzyme that helps in the formation of DNA from deoxyribonucleotide, a unit structure of a DNA. This enzyme helps in replication of the DNA strand to form the double helical structure of the DNA. DNA Polymerase reads the existing strand of DNA and synthesizes the new strand which is a replica of the existing strand.

RNA Polymerase is an enzyme that initiates RNA synthesis from a DNA template. This RNA polymerase enzyme opens the double-stranded DNA to expose one strand of the nucleotide so that it can be used as the template for RNA Synthesis.

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a) What are the differences between the Direct & Indirect Immunofluorescence Assays? (0.5 mark) b) What are the advantages of the Indirect Immunofluorescence Assays? (0.5 mark) c) A Super-Resolution Imaging Technique was developed in 2018 using imidazole, a His-tag ligand conjugated with a fluorophore to report the presence of a recombinant His-tag protein target, (Sci Rep, 2018, 8:5507). How does this technique improve the image quality? (2 marks)
a) What are the differences between the Direct & Indirect Immunofluorescence Assays?  b) What are the advantages of the Indirect Immunofluorescence Assays?  c) A Super-Resolution Imaging Technique was developed in 2018 using imidazole, a His-tag ligand conjugated with a fluorophore to report the presence of a recombinant His-tag protein target, (Sci Rep, 2018, 8:5507). How does this technique improve the image quality?
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