Chapter 4, Problem 50P

Interpretation Introduction

Interpretation:

The reason for the mixture of poly(A), poly(B) and the three DNAs shown in the curve to vary with respect to the C₀t value needs to be explained.

Concept introduction:

The C₀t value analysis is a technique used to measure the complexity in the size of DNA or gene and this technique is based on DNA renaturation kinetics.

This technique states the following principles:

• Renaturation rate is directly proportional to the number of times the sequences exist on the DNA Strand.
• When enough time is given all the denatured DNA are re-associated or reannealed.
• Renaturation takes the least time when there is more repetition of the sequences.

The renaturation process carried out by heating the DNA and allowing it to cool down to reanneal.

The C₀t value depends on − DNA Concentration, reassociation temperature, cation concentration, and viscosity and is given by the equation:

${\text{C}}_{\text{0}}\text{t value = DNA Concentration }\left(\text{moles/L}\right)\times \text{Renaturation time }\left(\text{Secs}\right)\times \text{Buffer Factor}$

Lower C₀t value shows more repetition of sequences while Higher C₀t value indicates a greater number of unique sequences.