
Concept explainers
(a)
Interpretation:
The type of the radioactive material needs to be determined that is added to the culture medium considering that DNA is supposed to be radioactively labeled but not RNA, in dividing and growing bacterial cell.
Concept introduction:
Nucleotides are the construction buildings of
(b)
Interpretation:
A product comprising DNA polymerase and primed model DNA must be supplemented with precursors. Also, where core phosphorous atoms are evenly marked with 32P, the location of radioactive atoms in these precursors when the DNA is to be ready should be stated.
Concept introduction:
DNA elongation performs in 5’-3’direction. Chain elongation reaction takes place when 3-hydroxyl group attacks the innermost phosphorus atom of deoxy nucleoside triphosphate. In the

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Chapter 4 Solutions
Biochemistry (Looseleaf)
- Imagine that aldolase can react with the seven carbon molecule Sedoheptulose-1,7-bisphosphate (below). Use the mechanism to predict the two products generated. Please draw out the stereochemistry in a fischer projection.arrow_forwardSodium borohydride (NaBH4) is a potent inhibitor of aldolase. It is known to ONLY inhibit theenzyme when it is complexed with substrate. Treatment of the enzyme alone has no effect.What is the mechanism for this inhibition? Please draw out the mechanism and show how it inhibits this.arrow_forwardShow the fate of the proton on the 4-Oxygen molecule of F-1,6-BP. Please include a drawing showing the electron flow that occurs.arrow_forward
- 1. Which one is the major organic product obtained from the following aldol condensation? O NaOH, H₂O heat A B C D Earrow_forwardAn organic chemist ordered the wrong item. She wanted to obtain 1-hydroxy-2-butanone, butinstead ordered 2-hydroxybutyraldehyde. As a good biochemist, show how the organic chemistcould use biological catalysis to make her desired compound. Please show the mechanism by drawing.arrow_forwardShow the fate of the hydrogen on carbon-2 of glucose. Please draw out the structure using curve arrows to show electron flow.arrow_forward
- 3. Which one of the compounds below is the major product formed by the reaction sequence shown here? CH3 + CH3NO2 NaOH H2, Ni ? nitromethane acetophenone OH OH HO HN- u x x x x Ph A HO -NH2 HO H Ph Ph Ph N- H B Ph NH2 D Earrow_forward4. Only ONE of the five compounds below can be prepared by an aldol condensation in which a single carbonyl compound is treated with base. Which one is it? To solve this problem, reverse the aldol condensation that formed each of these molecules to find out what two molecules came together to make the products. The one in which the two molecules are identical is the answer. Ph Ph ཚིག གནས ག ནཱ ཀ ན ཀནཱ A Ph H B Ph Ph H D Ph. Ph Ph E Harrow_forward5. Which one is the major organic product obtained from the following reaction sequence? First, equimolar amounts of cyclopentanone and LDA are mixed at -78°C. Then propionaldehyde (propanal) is added. Addition of aqueous acid completes the process. LDA, -78°C. 1. 2. H₂O* H A B H 0 D H H Earrow_forward
- 2. Which one is the major organic product obtained from the following reaction? NaOH, H₂O heat A B C D Earrow_forwardCH3CH2CHO + propanal PhCH2CHO 2-phenylacetaldehyde mixture of four products NaOH 10. In the crossed aldol reaction of propanal and 2-phenylacetaldehyde shown above, a mixture of four products will be formed. Which ONE of the compounds below will NOT be formed in this crossed aldol reaction? OH Ph A H OH OH Ph H B OH OH H H H Ph Ph C Ph D Earrow_forwardAn organic chemist ordered the wrong item. She wanted to obtain 1-hydroxy-2-butanone, butinstead ordered 2-hydroxybutyraldehyde. As a good biochemist, show how the organic chemistcould use biological catalysis to make her desired compound.arrow_forward
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